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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL826
Library AF
(Link to library)
Clone ID AFL826
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFL826P
(Link to Original site)
Representative DNA sequence 
>AFL826 (AFL826Q) /CSM/AF/AFL8-B/AFL826Q.Seq.d/
AAAATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACA
TGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAG
TTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGC
ATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATG
AAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAAT
TCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAAC
AAAAAGTTGATTGTGGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCA
GCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCA
AAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATC
AACAAAGTTGAAACCGATCAATTAACTCATCAATACACXXXXXXXXXXCNCCAGATANCA
CCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTACCCTCGCTGATGATTGGG
AATTACTTCCATCAAANCAAATCAAAGATCCAAANCAATCCAAACCAGTCGATTGGGTTG
ACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAG
CTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTG
AATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAA
TCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACG
CTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGC
AAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTA
AAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACG
ACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAA
AAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAG
ATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCA
GACGAACTTTAAANAAATAAATAA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
kxiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvig
ikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*RMN
KKLIVGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEI
NKVETDQLTHQY---

---PDXTYKVLVDNKEIQAGTLADDWELLPSXQIKDPXQSKPVDWVDVKEIDDPEDVKPA
GHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPL
IDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEA
EKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKK
STKKVIRRTLXK*i
Translated Amino Acid sequence (All Frames) 
Frame A:
kxiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvig
ikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*RMN
KKLIVGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEI
NKVETDQLTHQY---

---xqixptkyssitkrskqvpslmignyfhqxkskiqxnpnqsiglt*rksmtqkmlnq
pvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth
*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkk
pkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksk
nqpkkxsdel*xnk*

Frame B:
kxsyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la
*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*t
ks*lwvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkks
tklkpin*lint---

---xrxhlqstrr*qrdpsrypr**lgitsikxnqrskxiqtsrlg*rkgnr*prrc*ts
rs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpi
n**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprs
rkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqk
inqksxqtnfkxin

Frame C:
nxhiynyiffv**krkntnmrlllcliflvfvfnfalstvhfkdtfdndwesrwvvsdwh
kedgksgklvhtagkwfgdenqkgiqtsedarfyavsakfpsfsnkgkdlvlqytvkneq
kvdcgwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernq
qs*nrsinssih---

---PDXTYKVLVDNKEIQAGTLADDWELLPSXQIKDPXQSKPVDWVDVKEIDDPEDVKPA
GHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPL
IDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEA
EKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKK
STKKVIRRTLXK*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL826 (AFL826Q) /CSM/AF/AFL8-B/AFL826Q.Seq.d/                       1990   0.0
AFD172 (AFD172Q) /CSM/AF/AFD1-C/AFD172Q.Seq.d/                       1098   0.0
AFI321 (AFI321Q) /CSM/AF/AFI3-A/AFI321Q.Seq.d/                       1092   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1090   0.0
SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1090   0.0
AFO794 (AFO794Q) /CSM/AF/AFO7-D/AFO794Q.Seq.d/                       1090   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1090   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1090   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1090   0.0
AFI574 (AFI574Q) /CSM/AF/AFI5-D/AFI574Q.Seq.d/                       1090   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346543) Dictyostelium discoideum cDNA clone:dda24d08, 3' ...  1100   0.0    1
(BJ341682) Dictyostelium discoideum cDNA clone:dda7o17, 3' e...  1100   0.0    2
(BJ345638) Dictyostelium discoideum cDNA clone:dda28g07, 3' ...  1094   0.0    1
(BJ343770) Dictyostelium discoideum cDNA clone:dda17j05, 3' ...  1094   0.0    1
(BJ340935) Dictyostelium discoideum cDNA clone:dda4p12, 3' e...  1094   0.0    1
(BJ431077) Dictyostelium discoideum cDNA clone:ddv9e22, 3' e...  1092   0.0    2
(BJ400978) Dictyostelium discoideum cDNA clone:dds15m17, 3' ...  1092   0.0    2
(BJ346438) Dictyostelium discoideum cDNA clone:dda30l23, 3' ...  1092   0.0    1
(BJ346099) Dictyostelium discoideum cDNA clone:dda29i02, 3' ...  1092   0.0    1
(BJ345182) Dictyostelium discoideum cDNA clone:dda21k22, 3' ...  1092   0.0    2
dna update 2009. 4. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   503   e-141
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   195   e-100
BC068336_1(BC068336|pid:none) Danio rerio calreticulin, mRNA (cD...   202   2e-95
JH0795(JH0795;B31409;F60977) calreticulin precursor - California...   204   3e-95
AF195882_1(AF195882|pid:none) Danio rerio calreticulin mRNA, com...   199   2e-94
AC147179_27(AC147179|pid:none) Medicago truncatula clone mth2-14...   187   1e-91
AY271306_1(AY271306|pid:none) Dermacentor variabilis calreticuli...   194   3e-91
AY342298_1(AY342298|pid:none) Ictalurus punctatus ER-resident ch...   201   5e-91
AY395247_1(AY395247|pid:none) Amblyomma brasiliense calreticulin...   194   6e-91
AF177915_1(AF177915|pid:none) Strongylocentrotus purpuratus calr...   191   1e-90
protein update 2009. 4. 4
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFL826 is

5' end seq. ID AFL826F
5' end seq. 
>AFL826F.Seq
AAAATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACA
TGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAG
TTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGC
ATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATG
AAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAAT
TCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAAC
AAAAAGTTGATTGTGGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCA
GCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCA
AAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATC
AACAAAGTTGAAACCGATCAATTAACTCATCAATACACXXXXXXXXXX
Length of 5' end seq. 588
3' end seq. ID AFL826Z
3' end seq. 
>AFL826Z.Seq
XXXXXXXXXXCNCCAGATANCACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCA
GGTACCCTCGCTGATGATTGGGAATTACTTCCATCAAANCAAATCAAAGATCCAAANCAA
TCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCA
GCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGAT
TGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAA
GGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCA
TTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGT
GCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTC
ACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAA
GCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCT
GACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAA
GAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAA
AAATCAACCAAAAAAGTNATCAGACGAACTTTAAANAAATAAATAA
Length of 3' end seq. 766
Connected seq. ID AFL826P
Connected seq. 
>AFL826P.Seq
AAAATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACA
TGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAG
TTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGC
ATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATG
AAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAAT
TCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAAC
AAAAAGTTGATTGTGGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCA
GCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCA
AAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATC
AACAAAGTTGAAACCGATCAATTAACTCATCAATACAC----------CNCCAGATANCA
CCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTACCCTCGCTGATGATTGGG
AATTACTTCCATCAAANCAAATCAAAGATCCAAANCAATCCAAACCAGTCGATTGGGTTG
ACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAG
CTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTG
AATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAA
TCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACG
CTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGC
AAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTA
AAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACG
ACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAA
AAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAG
ATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCA
GACGAACTTTAAANAAATAAATAA
Length of connected seq. 1334
Full length Seq ID -
Full length Seq. -
Length of full length seq. -