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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB113
Library VF
(Link to library)
Clone ID VFB113
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16478-1
Original site URL
Representative seq. ID VFB113P
(Link to Original site)
Representative DNA sequence 
>VFB113 (VFB113Q) /CSM/VF/VFB1-A/VFB113Q.Seq.d/
AATTTCTTACAAACAAATACCACTAGCATCAAAATGTCCTATCCACCAAACCAAGGTTAT
CCACCACAACAAGGTTATCCACCACAACAAGGTTATCCACCACAACAAGGCTATCCACCA
CAACAAGGTTACCCACCACAACAAGGCTACCCACCACAACAAGGTTATCCACCACAACAA
GGCTACCCACCACAACAAGGTTACCCACCACAACAAGGTTACCCACCACAACAAGGCTAC
CCACCACAACAAGGTTATCCACCACAAGGTTATCCACCACAACAAGGTTATCCACCAGTT
GGTGTACCAGTTGGTGTACCAGTTGGATTTGCACCAGGTATGGTAGTTGGATATCATCAA
GGTTACTTTGTTGGTACAATCACTCATGATTGTAAACATGATGCTGAAGTTTTAAGAAAA
GCAATGAAAGGTATTGGTACAAATGAATCTGATTTAATTAAAGTTTTAGCCAATAGAAAT
TGGGCTGAACGTGAACAAATCAAGAGAGAGTTCTCTGCCAAATATAGCAAAGATTTAATT
CAAGATATTAAATCTGAAACCAGTGGTAACTTTGAAAAATGTTTXXXXXXXXXXGAACCA
GCTCATTTCGATGTTGAACAAATTCACAGTGCTTGCGCTGGTGCAGGTACCAACGAGAAC
ACTATAATTGAAATTTTAGTTACCCGTAGTAATGTACAAATGGAATACATTAAACAAATC
TTCAAGAATAAACATGGTAAATCATTAAAAGATCGTCTTGAATCCGAAGCAAGTGGTGAC
TTTAAGAAATTATTAGAGAAACTCACTGAACCAAGAGATGAATCACCAGTCATCAACCCA
ATGCAAGTTTCAAAAGATGCTGAAGATCTCTACAAAGCTGGTGAAGGTAAAATTGGTACC
GACGAAAAGGAATTCATCAAAATTCTCACCTCTCGTTCATTACCACATATTGCTGCCGTC
GCTAGTGAATACATTAAACATCACAAAAAACACTCACTCATCAAAGCAATCGATTCTGAA
TTCTCTGGTTCAATTAAAACTGGTTTAATCGCTATCGTTACCTATGCTCTCAACCCATAT
GGTTATTTCGCTGAAATCTTAAACAAATCAATGAAAGGTGCTGGTACAAATGATAACAAA
CTCATTCGTACTGTTGTAACTCAAATGCACAATATGCCACAAATTAAAACTGCTTATTCA
ACTCTCTTCAAGAATTCATTAGCTCATATATTCAAGC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
NFLQTNTTSIKMSYPPNQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQ
GYPPQQGYPPQQGYPPQQGYPPQQGYPPQGYPPQQGYPPVGVPVGVPVGFAPGMVVGYHQ
GYFVGTITHDCKHDAEVLRKAMKGIGTNESDLIKVLANRNWAEREQIKREFSAKYSKDLI
QDIKSETSGNFEKC---

---EPAHFDVEQIHSACAGAGTNENTIIEILVTRSNVQMEYIKQIFKNKHGKSLKDRLES
EASGDFKKLLEKLTEPRDESPVINPMQVSKDAEDLYKAGEGKIGTDEKEFIKILTSRSLP
HIAAVASEYIKHHKKHSLIKAIDSEFSGSIKTGLIAIVTYALNPYGYFAEILNKSMKGAG
TNDNKLIRTVVTQMHNMPQIKTAYSTLFKNSLAHIFK
Translated Amino Acid sequence (All Frames) 
Frame A:
NFLQTNTTSIKMSYPPNQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQ
GYPPQQGYPPQQGYPPQQGYPPQQGYPPQGYPPQQGYPPVGVPVGVPVGFAPGMVVGYHQ
GYFVGTITHDCKHDAEVLRKAMKGIGTNESDLIKVLANRNWAEREQIKREFSAKYSKDLI
QDIKSETSGNFEKC---

---EPAHFDVEQIHSACAGAGTNENTIIEILVTRSNVQMEYIKQIFKNKHGKSLKDRLES
EASGDFKKLLEKLTEPRDESPVINPMQVSKDAEDLYKAGEGKIGTDEKEFIKILTSRSLP
HIAAVASEYIKHHKKHSLIKAIDSEFSGSIKTGLIAIVTYALNPYGYFAEILNKSMKGAG
TNDNKLIRTVVTQMHNMPQIKTAYSTLFKNSLAHIFK

Frame B:
isykqiplaskcpihqtkvihhnkvihhnkvihhnkaihhnkvthhnkathhnkvihhnk
athhnkvthhnkvthhnkathhnkvihhkvihhnkvihqlvyqlvyqldlhqvw*ldiik
vtllvqslmivnmmlkf*ekq*kvlvqmnli*lkf*pieiglnvnksresslpniaki*f
kilnlkpvvtlknv---

---nqlismlnkftvlalvqvptrtl*lkf*lpvvmykwntlnkssrinmvnh*kivlnp
kqvvtlrny*rnslnqemnhqsstqckfqkmlkistklvkvklvptkrnsskfsplvhyh
illpslvntlnitknthsskqsilnslvqlklv*slslpmlsthmvislks*tnq*kvlv
qmitnsfvll*lkctichklklliqlssrih*liyss

Frame C:
fltnkyh*hqnvlstkprlstttrlstttrlstttrlstttrlptttrlptttrlstttr
lptttrlptttrlptttrlptttrlsttrlstttrlstswctswctswictrygswissr
llcwynhs*l*t*c*sfkksnerywyk*i*fn*sfsq*klg*t*tnqervlcqi*qrfns
ry*i*nqw*l*kmf---

---tssfrc*tnsqclrwcryqrehyn*nfsyp**ctngih*tnlqe*tw*iikrss*ir
skw*l*eiireth*tkr*itshqpnasfkrc*rslqsw*r*nwyrrkgihqnshlsfitt
yccrr**ih*tsqktlthqsnrf*ilwfn*nwfnryrylcsqpiwlfr*nlkqinercwy
k**qthsyccnsnaqyatn*nclfnslqefissyiq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB113 (VFB113Q) /CSM/VF/VFB1-A/VFB113Q.Seq.d/                       2077   0.0
VFA482 (VFA482Q) /CSM/VF/VFA4-D/VFA482Q.Seq.d/                       1273   0.0
VFH585 (VFH585Q) /CSM/VF/VFH5-D/VFH585Q.Seq.d/                       1267   0.0
VFL384 (VFL384Q) /CSM/VF/VFL3-D/VFL384Q.Seq.d/                       1261   0.0
VFI545 (VFI545Q) /CSM/VF/VFI5-B/VFI545Q.Seq.d/                       1261   0.0
SLA785 (SLA785Q) /CSM/SL/SLA7-D/SLA785Q.Seq.d/                       1261   0.0
VFD876 (VFD876Q) /CSM/VF/VFD8-D/VFD876Q.Seq.d/                       1257   0.0
VFL667 (VFL667Q) /CSM/VF/VFL6-C/VFL667Q.Seq.d/                       1253   0.0
VFK891 (VFK891Q) /CSM/VF/VFK8-D/VFK891Q.Seq.d/                       1253   0.0
VFF747 (VFF747Q) /CSM/VF/VFF7-B/VFF747Q.Seq.d/                       1253   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X60270|X60270.1 D.discoideum DdANN7 mRNA for annexin VII, partial cds.  1255  0.0  6
M69022|M69022.1 D.discoideum annexin VII (synexin) mRNA, complete cds.  1239  0.0  6
X60269|X60269.1 D.discoideum DdANN7 gene for annexin VII, partial cds.   842  0.0  7
BH161173|BH161173.1 ENTTB30TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  5e-29  8
U03413|U03413.1 Dictyostelium discoideum AX2 calcium binding protein mRNA, complete cds.    38  1e-18  8
X54016|X54016.1 D.discoideum mRNA for 24 kDa protein, homologous to C-terminal  repeat sequence of rhodopsin and synaptophysin.    94  3e-17  3
X54062|X54062.1 Dictyostelium discoideum CABP1-related gene.      94  3e-17  3
AY007806|AY007806.1 Dictyostelium discoideum comitin gene, complete cds.    94  3e-17  3
CA409753|CA409753.1 1303_F -P proteoid roots 12 and 14 DAE Lupinus albus cDNA clone 1303 5', mRNA sequence.   100  8e-17  2
AZ545567|AZ545567.1 ENTEX13TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  3e-16  6
dna update 2003. 8.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P24639) RecName: Full=Annexin A7; AltName: Full=Annexin-7; AltN...   585   e-165
M69022_1(M69022|pid:none) D.discoideum annexin VII (synexin) mRN...   583   e-165
BC068035_1(BC068035|pid:none) Xenopus tropicalis annexin A7, mRN...   221   3e-56
BC129692_1(BC129692|pid:none) Xenopus laevis hypothetical protei...   219   1e-55
BT059478_1(BT059478|pid:none) Salmo salar clone ssal-rgf-513-011...   216   1e-54
AF280423_1(AF280423|pid:none) Rattus norvegicus annexin VII mRNA...   216   2e-54
BC070896_1(BC070896|pid:none) Rattus norvegicus annexin A7, mRNA...   215   2e-54
(P26256) RecName: Full=Annexin-B12; AltName: Full=Annexin-12; Al...   215   2e-54
FJ803366_1(FJ803366|pid:none) Ictalurus punctatus annexin A11 mR...   213   9e-54
AK312447_1(AK312447|pid:none) Homo sapiens cDNA, FLJ92798, highl...   213   9e-54
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.19  alm: 0.33  top: 0.53  tms: 0.00  mit: -0.07  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: vacuolar
	  8.0 %: vesicles of secretory system
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VFB113 is nuc
5' end seq. ID VFB113F
5' end seq. 
>VFB113F.Seq
AATTTCTTACAAACAAATACCACTAGCATCAAAATGTCCTATCCACCAAACCAAGGTTAT
CCACCACAACAAGGTTATCCACCACAACAAGGTTATCCACCACAACAAGGCTATCCACCA
CAACAAGGTTACCCACCACAACAAGGCTACCCACCACAACAAGGTTATCCACCACAACAA
GGCTACCCACCACAACAAGGTTACCCACCACAACAAGGTTACCCACCACAACAAGGCTAC
CCACCACAACAAGGTTATCCACCACAAGGTTATCCACCACAACAAGGTTATCCACCAGTT
GGTGTACCAGTTGGTGTACCAGTTGGATTTGCACCAGGTATGGTAGTTGGATATCATCAA
GGTTACTTTGTTGGTACAATCACTCATGATTGTAAACATGATGCTGAAGTTTTAAGAAAA
GCAATGAAAGGTATTGGTACAAATGAATCTGATTTAATTAAAGTTTTAGCCAATAGAAAT
TGGGCTGAACGTGAACAAATCAAGAGAGAGTTCTCTGCCAAATATAGCAAAGATTTAATT
CAAGATATTAAATCTGAAACCAGTGGTAACTTTGAAAAATGTTT----------
Length of 5' end seq. 584
3' end seq. ID VFB113Z
3' end seq. 
>VFB113Z.Seq
----------GAACCAGCTCATTTCGATGTTGAACAAATTCACAGTGCTTGCGCTGGTGC
AGGTACCAACGAGAACACTATAATTGAAATTTTAGTTACCCGTAGTAATGTACAAATGGA
ATACATTAAACAAATCTTCAAGAATAAACATGGTAAATCATTAAAAGATCGTCTTGAATC
CGAAGCAAGTGGTGACTTTAAGAAATTATTAGAGAAACTCACTGAACCAAGAGATGAATC
ACCAGTCATCAACCCAATGCAAGTTTCAAAAGATGCTGAAGATCTCTACAAAGCTGGTGA
AGGTAAAATTGGTACCGACGAAAAGGAATTCATCAAAATTCTCACCTCTCGTTCATTACC
ACATATTGCTGCCGTCGCTAGTGAATACATTAAACATCACAAAAAACACTCACTCATCAA
AGCAATCGATTCTGAATTCTCTGGTTCAATTAAAACTGGTTTAATCGCTATCGTTACCTA
TGCTCTCAACCCATATGGTTATTTCGCTGAAATCTTAAACAAATCAATGAAAGGTGCTGG
TACAAATGATAACAAACTCATTCGTACTGTTGTAACTCAAATGCACAATATGCCACAAAT
TAAAACTGCTTATTCAACTCTCTTCAAGAATTCATTAGCTCATATATTCAAGC
Length of 3' end seq. 643
Connected seq. ID VFB113P
Connected seq. 
>VFB113P.Seq
AATTTCTTACAAACAAATACCACTAGCATCAAAATGTCCTATCCACCAAACCAAGGTTAT
CCACCACAACAAGGTTATCCACCACAACAAGGTTATCCACCACAACAAGGCTATCCACCA
CAACAAGGTTACCCACCACAACAAGGCTACCCACCACAACAAGGTTATCCACCACAACAA
GGCTACCCACCACAACAAGGTTACCCACCACAACAAGGTTACCCACCACAACAAGGCTAC
CCACCACAACAAGGTTATCCACCACAAGGTTATCCACCACAACAAGGTTATCCACCAGTT
GGTGTACCAGTTGGTGTACCAGTTGGATTTGCACCAGGTATGGTAGTTGGATATCATCAA
GGTTACTTTGTTGGTACAATCACTCATGATTGTAAACATGATGCTGAAGTTTTAAGAAAA
GCAATGAAAGGTATTGGTACAAATGAATCTGATTTAATTAAAGTTTTAGCCAATAGAAAT
TGGGCTGAACGTGAACAAATCAAGAGAGAGTTCTCTGCCAAATATAGCAAAGATTTAATT
CAAGATATTAAATCTGAAACCAGTGGTAACTTTGAAAAATGTTT----------GAACCA
GCTCATTTCGATGTTGAACAAATTCACAGTGCTTGCGCTGGTGCAGGTACCAACGAGAAC
ACTATAATTGAAATTTTAGTTACCCGTAGTAATGTACAAATGGAATACATTAAACAAATC
TTCAAGAATAAACATGGTAAATCATTAAAAGATCGTCTTGAATCCGAAGCAAGTGGTGAC
TTTAAGAAATTATTAGAGAAACTCACTGAACCAAGAGATGAATCACCAGTCATCAACCCA
ATGCAAGTTTCAAAAGATGCTGAAGATCTCTACAAAGCTGGTGAAGGTAAAATTGGTACC
GACGAAAAGGAATTCATCAAAATTCTCACCTCTCGTTCATTACCACATATTGCTGCCGTC
GCTAGTGAATACATTAAACATCACAAAAAACACTCACTCATCAAAGCAATCGATTCTGAA
TTCTCTGGTTCAATTAAAACTGGTTTAATCGCTATCGTTACCTATGCTCTCAACCCATAT
GGTTATTTCGCTGAAATCTTAAACAAATCAATGAAAGGTGCTGGTACAAATGATAACAAA
CTCATTCGTACTGTTGTAACTCAAATGCACAATATGCCACAAATTAAAACTGCTTATTCA
ACTCTCTTCAAGAATTCATTAGCTCATATATTCAAGC
Length of connected seq. 1227
Full length Seq ID -
Full length Seq. -
Length of full length seq. -