>VFC221 (VFC221Q) /CSM/VF/VFC2-A/VFC221Q.Seq.d/
XXXXXXXXXXACAATGGTGACTATTTCCCATTATGGGGAACATGCATGGGATTCCAAGAG
TTGGCATTATTATCAGCAGACAATTTCAATTTACTCTCATCCTACAATTCAGAAAACTAC
ACTGTACCATTAAACTTCACATCATTAGCAGCCGGTAGTAGATTATTCTCATTGGCATCA
TCAAGCATTATGCAATCATTAGCAAGCGAACCAATTACAATGAATAATCATCAATTTGGT
TTATCACCACAAACCTATCAACAAACATCATCAATCAATACTTTCTTTGATGTTTTATCA
ACCAATGTAGATCGTGATGGTAATACCTTTATCTCTACCATCGAGGCAAAGAACTATCCA
ATCTATGGTACTCAATGGCATCCAGAGAAACCAATTTTCGAATGGTGGGATCAAGAAGTA
ATGAATCATTCATTCGACTCAATCATGGCCAATCAATACACTTCAAACTTTTTCGTTAAT
GAATGTAGAAAATCACTTCACTCTTT

---NGDYFPLWGTCMGFQELALLSADNFNLLSSYNSENYTVPLNFTSLAAGSRLFSLASS
SIMQSLASEPITMNNHQFGLSPQTYQQTSSINTFFDVLSTNVDRDGNTFISTIEAKNYPI
YGTQWHPEKPIFEWWDQEVMNHSFDSIMANQYTSNFFVNECRKSLHS

Frame A:
---qw*lfpimgnmhgiprvgiiisrqfqftlilqfrklhctiklhiissr**iiligii
khyaiiskrtnyne*ssiwfittnlstniinqyfl*cfinqcrs*w*ylylyhrgkelsn
lwysmasretnfrmvgsrsnesfirlnhgqsihfklfr**m*kitslf


Frame B:
---NGDYFPLWGTCMGFQELALLSADNFNLLSSYNSENYTVPLNFTSLAAGSRLFSLASS
SIMQSLASEPITMNNHQFGLSPQTYQQTSSINTFFDVLSTNVDRDGNTFISTIEAKNYPI
YGTQWHPEKPIFEWWDQEVMNHSFDSIMANQYTSNFFVNECRKSLHS


Frame C:
---mvtishygehawdskswhyyqqtisiyshptiqkttlyh*tshh*qpvvdyshwhhq
alcnh*qanqlq*iiinlvyhhkpinkhhqsilslmfyqpm*ivmviplslpsrqrtiqs
mvlngiqrnqfsnggikk**iihstqswpintlqtfslmnvenhftl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFC221 (VFC221Q) /CSM/VF/VFC2-A/VFC221Q.Seq.d/                        983   0.0
VFN417 (VFN417Q) /CSM/VF/VFN4-A/VFN417Q.Seq.d/                        975   0.0
VFK141 (VFK141Q) /CSM/VF/VFK1-B/VFK141Q.Seq.d/                        967   0.0
VSJ794 (VSJ794Q) /CSM/VS/VSJ7-D/VSJ794Q.Seq.d/                        955   0.0
SSJ116 (SSJ116Q) /CSM/SS/SSJ1-A/SSJ116Q.Seq.d/                        581   e-165
SSJ121 (SSJ121Q) /CSM/SS/SSJ1-A/SSJ121Q.Seq.d/                        339   2e-92
VSE226 (VSE226Q) /CSM/VS/VSE2-B/VSE226Q.Seq.d/                         40   0.032
SSG302 (SSG302Q) /CSM/SS/SSG3-A/SSG302Q.Seq.d/                         40   0.032
SLG828 (SLG828Q) /CSM/SL/SLG8-B/SLG828Q.Seq.d/                         40   0.032
SLG521 (SLG521Q) /CSM/SL/SLG5-A/SLG521Q.Seq.d/                         40   0.032

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AP001861|AP001861.1 Homo sapiens genomic DNA, chromosome 4q22-q24, clone:2136P7, WORKING DRAFT SEQUENCE, unordered pieces.    44  1.6  1
AL161572|AL161572.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 68.    32  2.1  6
AC079370|AC079370.2 Mus musculus chromosome 12 clone RP23-34L6 strain C57BL6/J, WORKING DRAFT SEQUENCE, 19 unordered pieces.    42  6.5  1
AC131782|AC131782.2 Mus musculus chromosome UNK clone RP24-361A10, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42  6.5  1
AE017006|AE017006.1 Bacillus cereus ATCC 14579 section 9 of 18 of the complete genome.    34  9.2  6
AC058823|AC058823.4 Homo sapiens BAC clone RP11-810D13 from 4, complete sequence.    44  9.5  2
AC104622|AC104622.3 Homo sapiens BAC clone RP11-481L3 from 2, complete sequence.    36  9.6  3
AF399011|AF399011.1 Pseudomonas phage phiKZ, complete genome.     44   1115  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54HL4) RecName: Full=Gamma-glutamyl hydrolase B;          EC=3...   206   3e-52
BC108438_1(BC108438|pid:none) Xenopus laevis hypothetical LOC496...   164   9e-40
BC076459_1(BC076459|pid:none) Danio rerio hypothetical protein L...   155   7e-37
BC093328_1(BC093328|pid:none) Danio rerio hypothetical protein L...   155   7e-37
BC154502_1(BC154502|pid:none) Danio rerio hypothetical protein L...   155   7e-37
BC074274_1(BC074274|pid:none) Xenopus laevis MGC84044 protein, m...   152   3e-36
BC124118_1(BC124118|pid:none) Danio rerio hypothetical protein L...   150   1e-35
BT075280_1(BT075280|pid:none) Osmerus mordax clone omor-eva-514-...   147   2e-34
BC153967_1(BC153967|pid:none) Danio rerio zgc:171566, mRNA (cDNA...   146   2e-34
BT048619_1(BT048619|pid:none) Salmo salar clone ssal-rgb2-633-16...   144   1e-33

 psg: 0.75  gvh: 0.64  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.11  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: peroxisomal
	  4.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for VFC221 is cyt

>VFC221Z.Seq
----------ACAATGGTGACTATTTCCCATTATGGGGAACATGCATGGGATTCCAAGAG
TTGGCATTATTATCAGCAGACAATTTCAATTTACTCTCATCCTACAATTCAGAAAACTAC
ACTGTACCATTAAACTTCACATCATTAGCAGCCGGTAGTAGATTATTCTCATTGGCATCA
TCAAGCATTATGCAATCATTAGCAAGCGAACCAATTACAATGAATAATCATCAATTTGGT
TTATCACCACAAACCTATCAACAAACATCATCAATCAATACTTTCTTTGATGTTTTATCA
ACCAATGTAGATCGTGATGGTAATACCTTTATCTCTACCATCGAGGCAAAGAACTATCCA
ATCTATGGTACTCAATGGCATCCAGAGAAACCAATTTTCGAATGGTGGGATCAAGAAGTA
ATGAATCATTCATTCGACTCAATCATGGCCAATCAATACACTTCAAACTTTTTCGTTAAT
GAATGTAGAAAATCACTTCACTCTTT