NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD746
Library VF
(Link to library)
Clone ID VFD746
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15827-1
Original site URL
Representative seq. ID VFD746P
(Link to Original site)
Representative DNA sequence 
>VFD746 (VFD746Q) /CSM/VF/VFD7-B/VFD746Q.Seq.d/
AAATCAAGTGAAAAATCAACACAAGAAGTTGAACAAGTTATTAAACCAGAAAAAACCCCA
ATTTTAGATACTAGTAAATGGCCATTATTATTAAAAAATTATGATCAATTATCAGTTAGA
ACTGGTCATTATACACCAATTCCAAATGGTCATTCACCATTAAAGAGACCAATCAAAGAG
TATGTTAAATATGGTATTATCAATTTGGATAAACCATCAAATCCATCATCACATGAAGTT
GTAGCATGGATTCGTACCATTCTTCGTGTAACCAAAACTGGTCACTCTGGTACATTGGAT
CCAAAGGTTACTGGTTGTTTAATCGTTTGTATTGAACGTGCAACTCGTTTGGTTAAATCA
CAACAAGGTGCAGGTAAAGAGTACATTGGTATCGTTCGTTTACATGGTGCCATCGAAGGT
ACCACTGAACTCTCAAAGGCTGTAGACACCTTGACCGGTGCTCTCTTCCAAAGACCACCA
CAAAAATCTGCCGTCAAAAAGAGATTAAGAGTTCGTACCATTCATAACTCCAAATTATTA
GAATTTGACCCAGAACGTAATTTAGGTTTAATTTGGGTCGATTGTGXXXXXXXXXXAGCC
GGTACCTATATTCGTACCCTTTGCGTTCACATTGGTCTCTTGATGGGTGTCGGTGGTCAT
ATGCAAGAACTTCGTAGAGTCCGTTCCGGTATCATGTCTGAAAAGAAGGGTCAAGTCACC
ATGCATGACGTCAAAGACGCTCAATACGAATATGACAATAGCAAAGACGAATCTTACTTA
CGTCGTGTCATTCAACCATTGGAAGCAATTCTCACCAATTACAAGAGAATCGTCGTTAAA
GACTCTGCAATCAATGCCATCTGTTATGGTGCTAAACTCATGATTCCAGGTCTCTTACGT
TATGAACAAGACATTGAATCAAACGAAGAAGTCGTTCTTATCACCACCAAAGGTGAAGCC
ATCGCTATTGGTATCGCTCAAATGACCACCGCTTCAATGGCCACTTGTGATCATGGTGTC
GTTGCCACAATCAAAAGAGTTATCATGGATAGAGATGTCTATCCAGTTAGATGGGGTCTT
GGTCCAAAAGCAAAGGTCAAGAAGGAAATGATCAAAGATGGTAAATTAGACAAATTTGGT
AAACCAAATGAAAAAACTCCATCAGATTGGACCAACACTTACACCTATTACACTGGTCAA
GTTGCCACTACCACCACCACCTCCCCAGTCGAATCAATGAATGTTGATACTCCAAAGAAA
GCAACTGCCTCTGTCGCCGTTAAAGC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KSSEKSTQEVEQVIKPEKTPILDTSKWPLLLKNYDQLSVRTGHYTPIPNGHSPLKRPIKE
YVKYGIINLDKPSNPSSHEVVAWIRTILRVTKTGHSGTLDPKVTGCLIVCIERATRLVKS
QQGAGKEYIGIVRLHGAIEGTTELSKAVDTLTGALFQRPPQKSAVKKRLRVRTIHNSKLL
EFDPERNLGLIWVDC---

---AGTYIRTLCVHIGLLMGVGGHMQELRRVRSGIMSEKKGQVTMHDVKDAQYEYDNSKD
ESYLRRVIQPLEAILTNYKRIVVKDSAINAICYGAKLMIPGLLRYEQDIESNEEVVLITT
KGEAIAIGIAQMTTASMATCDHGVVATIKRVIMDRDVYPVRWGLGPKAKVKKEMIKDGKL
DKFGKPNEKTPSDWTNTYTYYTGQVATTTTTSPVESMNVDTPKKATASVAVK
Translated Amino Acid sequence (All Frames) 
Frame A:
KSSEKSTQEVEQVIKPEKTPILDTSKWPLLLKNYDQLSVRTGHYTPIPNGHSPLKRPIKE
YVKYGIINLDKPSNPSSHEVVAWIRTILRVTKTGHSGTLDPKVTGCLIVCIERATRLVKS
QQGAGKEYIGIVRLHGAIEGTTELSKAVDTLTGALFQRPPQKSAVKKRLRVRTIHNSKLL
EFDPERNLGLIWVDC---

---srylysyplrshwsldgcrwsyarts*spfryhv*kegsshha*rqrrsiri*q*qr
rilltschstigsnshqlqenrr*rlcnqchllwc*thdsrsltl*trh*ikrrsrsyhh
qr*shrywyrsndhrfnghl*swcrchnqksyhg*rclss*mgswskskgqegndqrw*i
rqiw*tk*knsirldqhlhllhwsschyhhhlpsrinec*yskesnclcrr*s

Frame B:
nqvknqhkklnkllnqkkpqf*ilvnghyy*kiminyqlelviihqfqmvihh*rdqsks
mlnmvlsiwinhqihhhmkl*hgfvpffv*pklvtlvhwiqrllvv*sfvlnvqlvwlnh
nkvqvkstlvsfvymvpskvplnsqrl*tp*pvlsskdhhknlpskrd*efvpfitpny*
nltqnvi*v*fgsiv---

---AGTYIRTLCVHIGLLMGVGGHMQELRRVRSGIMSEKKGQVTMHDVKDAQYEYDNSKD
ESYLRRVIQPLEAILTNYKRIVVKDSAINAICYGAKLMIPGLLRYEQDIESNEEVVLITT
KGEAIAIGIAQMTTASMATCDHGVVATIKRVIMDRDVYPVRWGLGPKAKVKKEMIKDGKL
DKFGKPNEKTPSDWTNTYTYYTGQVATTTTTSPVESMNVDTPKKATASVAVK

Frame C:
ik*kintrs*tsy*trknpnfry**maiiikkl*siis*nwslytnskwsftiketnqrv
c*iwyyqfg*tiksiit*scsmdsyhsscnqnwslwyigskgywlfnrly*tcnsfg*it
trcr*rvhwyrsftwchrryh*tlkgcrhldrcslpktttkicrqkeikssyhs*lqiir
i*prt*frfnlgrl---

---pvpifvpfaftlvs*wvsvvicknfvesvpvsclkrrvkspcmtsktlntnmtiakt
nltyvvsfnhwkqfspitresslktlqsmpsvmvlns*fqvsyvmnktlnqtkksflspp
kvkpsllvslk*pplqwplvimvslpqskelswiemsiqldgvlvqkqrsrrk*skmvn*
tnlvnqmkklhqigptltpitlvklplpppppqsnq*mlilqrkqlplsplk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD746 (VFD746Q) /CSM/VF/VFD7-B/VFD746Q.Seq.d/                       2490   0.0
VFB588 (VFB588Q) /CSM/VF/VFB5-D/VFB588Q.Seq.d/                       1342   0.0
VFD801 (VFD801Q) /CSM/VF/VFD8-A/VFD801Q.Seq.d/                       1287   0.0
VFG107 (VFG107Q) /CSM/VF/VFG1-A/VFG107Q.Seq.d/                       1215   0.0
SLA614 (SLA614Q) /CSM/SL/SLA6-A/SLA614Q.Seq.d/                       1191   0.0
CHB777 (CHB777Q) /CSM/CH/CHB7-D/CHB777Q.Seq.d/                       1134   0.0
VFC387 (VFC387Q) /CSM/VF/VFC3-D/VFC387Q.Seq.d/                       1116   0.0
SLG818 (SLG818Q) /CSM/SL/SLG8-A/SLG818Q.Seq.d/                       1057   0.0
VFG375 (VFG375Q) /CSM/VF/VFG3-D/VFG375Q.Seq.d/                       1041   0.0
VFE566 (VFE566Q) /CSM/VF/VFE5-C/VFE566Q.Seq.d/                        989   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX489105|AX489105.1 Sequence 6405 from Patent WO02053728.         86  2e-58  6
U59149|U59149.1 Candida albicans nucleolar protein CaCbf5p (CaCBF5) gene, complete cds.    86  1e-57  6
AX594914|AX594914.1 Sequence 568 from Patent EP1258494.           86  6e-40  8
L12351|L12351.1 Yeast centromere/microtubule binding protein (CBF5) gene, complete cds.    86  1e-37  8
AF008563|AF008563.1 Kluyveromyces lactis centromere-binding factor 5 (CBF5) gene, complete cds.    44  2e-32  10
U17246|U17246.1 Saccharomyces cerevisiae chromosome XII cosmid 9470.    86  2e-30  8
AL424495|AL424495.1 T3 end of clone AZ0AA014G02 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    74  1e-26  5
U59150|U59150.1 Sartorya fumigata nucleolar protein AfCbf5p (AfCBF5p) mRNA, complete cds.    56  2e-25  6
AL426547|AL426547.1 clone BA0AB013E12 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    40  9e-20  8
AZ927873|AZ927873.1 476.dis09b05.s1 Saccharomyces castellii NRRL Y-12630 Saccharomyces castellii genomic clone 476.dis09b05.s1, DNA sequence.    72  1e-19  3
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9LD90) RecName: Full=H/ACA ribonucleoprotein complex subunit 4...   583   e-165
BT065061_1(BT065061|pid:none) Zea mays full-length cDNA clone ZM...   576   e-163
AK166105_1(AK166105|pid:none) Mus musculus lung RCB-0558 LLC cDN...   572   e-162
BC099966_1(BC099966|pid:none) Mus musculus dyskeratosis congenit...   572   e-161
FJ236702_1(FJ236702|pid:none) Sus scrofa dyskeratosis congenita ...   571   e-161
BC123284_1(BC123284|pid:none) Xenopus laevis hypothetical protei...   570   e-161
BC108888_1(BC108888|pid:none) Xenopus laevis hypothetical protei...   570   e-161
(O14007) RecName: Full=H/ACA ribonucleoprotein complex subunit 4...   569   e-161
(O60832) RecName: Full=H/ACA ribonucleoprotein complex subunit 4...   567   e-160
AJ010395_1(AJ010395|pid:none) Homo sapiens DKC1 gene, exons 1 to...   567   e-160
protein update 2009. 6.18
PSORT 

 psg: 0.75  gvh: 0.28  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal

>> prediction for VFD746 is cyt
5' end seq. ID VFD746F
5' end seq. 
>VFD746F.Seq
AAATCAAGTGAAAAATCAACACAAGAAGTTGAACAAGTTATTAAACCAGAAAAAACCCCA
ATTTTAGATACTAGTAAATGGCCATTATTATTAAAAAATTATGATCAATTATCAGTTAGA
ACTGGTCATTATACACCAATTCCAAATGGTCATTCACCATTAAAGAGACCAATCAAAGAG
TATGTTAAATATGGTATTATCAATTTGGATAAACCATCAAATCCATCATCACATGAAGTT
GTAGCATGGATTCGTACCATTCTTCGTGTAACCAAAACTGGTCACTCTGGTACATTGGAT
CCAAAGGTTACTGGTTGTTTAATCGTTTGTATTGAACGTGCAACTCGTTTGGTTAAATCA
CAACAAGGTGCAGGTAAAGAGTACATTGGTATCGTTCGTTTACATGGTGCCATCGAAGGT
ACCACTGAACTCTCAAAGGCTGTAGACACCTTGACCGGTGCTCTCTTCCAAAGACCACCA
CAAAAATCTGCCGTCAAAAAGAGATTAAGAGTTCGTACCATTCATAACTCCAAATTATTA
GAATTTGACCCAGAACGTAATTTAGGTTTAATTTGGGTCGATTGTG----------
Length of 5' end seq. 586
3' end seq. ID VFD746Z
3' end seq. 
>VFD746Z.Seq
----------AGCCGGTACCTATATTCGTACCCTTTGCGTTCACATTGGTCTCTTGATGG
GTGTCGGTGGTCATATGCAAGAACTTCGTAGAGTCCGTTCCGGTATCATGTCTGAAAAGA
AGGGTCAAGTCACCATGCATGACGTCAAAGACGCTCAATACGAATATGACAATAGCAAAG
ACGAATCTTACTTACGTCGTGTCATTCAACCATTGGAAGCAATTCTCACCAATTACAAGA
GAATCGTCGTTAAAGACTCTGCAATCAATGCCATCTGTTATGGTGCTAAACTCATGATTC
CAGGTCTCTTACGTTATGAACAAGACATTGAATCAAACGAAGAAGTCGTTCTTATCACCA
CCAAAGGTGAAGCCATCGCTATTGGTATCGCTCAAATGACCACCGCTTCAATGGCCACTT
GTGATCATGGTGTCGTTGCCACAATCAAAAGAGTTATCATGGATAGAGATGTCTATCCAG
TTAGATGGGGTCTTGGTCCAAAAGCAAAGGTCAAGAAGGAAATGATCAAAGATGGTAAAT
TAGACAAATTTGGTAAACCAAATGAAAAAACTCCATCAGATTGGACCAACACTTACACCT
ATTACACTGGTCAAGTTGCCACTACCACCACCACCTCCCCAGTCGAATCAATGAATGTTG
ATACTCCAAAGAAAGCAACTGCCTCTGTCGCCGTTAAAGC
Length of 3' end seq. 690
Connected seq. ID VFD746P
Connected seq. 
>VFD746P.Seq
AAATCAAGTGAAAAATCAACACAAGAAGTTGAACAAGTTATTAAACCAGAAAAAACCCCA
ATTTTAGATACTAGTAAATGGCCATTATTATTAAAAAATTATGATCAATTATCAGTTAGA
ACTGGTCATTATACACCAATTCCAAATGGTCATTCACCATTAAAGAGACCAATCAAAGAG
TATGTTAAATATGGTATTATCAATTTGGATAAACCATCAAATCCATCATCACATGAAGTT
GTAGCATGGATTCGTACCATTCTTCGTGTAACCAAAACTGGTCACTCTGGTACATTGGAT
CCAAAGGTTACTGGTTGTTTAATCGTTTGTATTGAACGTGCAACTCGTTTGGTTAAATCA
CAACAAGGTGCAGGTAAAGAGTACATTGGTATCGTTCGTTTACATGGTGCCATCGAAGGT
ACCACTGAACTCTCAAAGGCTGTAGACACCTTGACCGGTGCTCTCTTCCAAAGACCACCA
CAAAAATCTGCCGTCAAAAAGAGATTAAGAGTTCGTACCATTCATAACTCCAAATTATTA
GAATTTGACCCAGAACGTAATTTAGGTTTAATTTGGGTCGATTGTG----------AGCC
GGTACCTATATTCGTACCCTTTGCGTTCACATTGGTCTCTTGATGGGTGTCGGTGGTCAT
ATGCAAGAACTTCGTAGAGTCCGTTCCGGTATCATGTCTGAAAAGAAGGGTCAAGTCACC
ATGCATGACGTCAAAGACGCTCAATACGAATATGACAATAGCAAAGACGAATCTTACTTA
CGTCGTGTCATTCAACCATTGGAAGCAATTCTCACCAATTACAAGAGAATCGTCGTTAAA
GACTCTGCAATCAATGCCATCTGTTATGGTGCTAAACTCATGATTCCAGGTCTCTTACGT
TATGAACAAGACATTGAATCAAACGAAGAAGTCGTTCTTATCACCACCAAAGGTGAAGCC
ATCGCTATTGGTATCGCTCAAATGACCACCGCTTCAATGGCCACTTGTGATCATGGTGTC
GTTGCCACAATCAAAAGAGTTATCATGGATAGAGATGTCTATCCAGTTAGATGGGGTCTT
GGTCCAAAAGCAAAGGTCAAGAAGGAAATGATCAAAGATGGTAAATTAGACAAATTTGGT
AAACCAAATGAAAAAACTCCATCAGATTGGACCAACACTTACACCTATTACACTGGTCAA
GTTGCCACTACCACCACCACCTCCCCAGTCGAATCAATGAATGTTGATACTCCAAAGAAA
GCAACTGCCTCTGTCGCCGTTAAAGC
Length of connected seq. 1276
Full length Seq ID -
Full length Seq. -
Length of full length seq. -