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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM417
Library AF
(Link to library)
Clone ID AFM417
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM417P
(Link to Original site)
Representative DNA sequence 
>AFM417 (AFM417Q) /CSM/AF/AFM4-A/AFM417Q.Seq.d/
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAAANTTAAAAANTCNNCTTTTTTGAAAAAAAAAA
XXXXXXXXXXGNNNNNNNNNNNNNTGGTANNNNNTNNNTNTTNNANTTNAGGNTNNNNTA
ATATTNNNNNTGTTNNNNNNAANATTNAANNNAAANAAGGTATNNCNNCANNNCANNAAA
NATTNATTTTNNNTGGTNNNCAATTANAANATGGTNGTNCTNTNTNTGATTNCAACATTC
AAAAGGAATNCNCTNTCCATTTAGTCNTCAGATTAAGAGGTGGTATGCAAATTTTTGTCA
AAACCCTCACTGGTAAGACTATTACACTNGAAGTTGAAGGTTCTGATAACATTGAGAATG
TTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCG
CCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCA
CTNTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTG
GTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAA
TTCAAGACAAAGAAGGTATTCCA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
ihfyytnikfifyffiycnis*KKKKKKKXKXSXFLKKK---

---XXXXXVXXXXXXXGXXNIXXVXXXIXXKXGXXXXXXXXIXXGXQLXXGXXXXDXNIQ
KEXXXHLVXRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKAKIQDKEGIPPDQQRLIFA
GKQLEDGRTLSDYNIQKESTXHLVLRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKTKI
QDKEGIP
Translated Amino Acid sequence (All Frames) 
Frame A:
kfifiiqilnsyfiflyivtfhkkkkkkkxlkxxlf*kkk---

---xxxxxgxxxxxxxrxx*yxxcxxxxxxxxryxxxxxxixfxwxxixxwxxxx*xqhs
kgxxxpfsxqikrwyanfcqnphw*dyytxs*rf**h*ec*sqnprqrrystrsttshfr
r*tiggwsysl*lqhskgihxpfssqikrwyanfcknshw*nhytrs*r**qh*ecknkn
srqrrys

Frame B:
nsfllyky*ihilffyil*hfikkkkkkkx*kxxffekk---

---XXXXXVXXXXXXXGXXNIXXVXXXIXXKXGXXXXXXXXIXXGXQLXXGXXXXDXNIQ
KEXXXHLVXRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKAKIQDKEGIPPDQQRLIFA
GKQLEDGRTLSDYNIQKESTXHLVLRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKTKI
QDKEGIP

Frame C:
ihfyytnikfifyffiycnis*KKKKKKKXKXSXFLKKK---

---xxxxwxxxxxxxxxxxixxxxxxxxxxxkvxxxxxkxxfxxvxnxxmvvlxxixtfk
rnxlsi*ssd*evvckflskpslvrllhxklkvlitlrmlkpksktkkvfhqinnvsfsp
vnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkf
ktkkvf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK183 (VFK183Q) /CSM/VF/VFK1-D/VFK183Q.Seq.d/                        755   0.0
SFK754 (SFK754Q) /CSM/SF/SFK7-C/SFK754Q.Seq.d/                        755   0.0
SFI843 (SFI843Q) /CSM/SF/SFI8-B/SFI843Q.Seq.d/                        755   0.0
SFI410 (SFI410Q) /CSM/SF/SFI4-A/SFI410Q.Seq.d/                        755   0.0
SFI270 (SFI270Q) /CSM/SF/SFI2-C/SFI270Q.Seq.d/                        755   0.0
SFG820 (SFG820Q) /CSM/SF/SFG8-A/SFG820Q.Seq.d/                        755   0.0
SFD815 (SFD815Q) /CSM/SF/SFD8-A/SFD815Q.Seq.d/                        755   0.0
SFD545 (SFD545Q) /CSM/SF/SFD5-B/SFD545Q.Seq.d/                        755   0.0
SFB478 (SFB478Q) /CSM/SF/SFB4-D/SFB478Q.Seq.d/                        755   0.0
CHD793 (CHD793Q) /CSM/CH/CHD7-D/CHD793Q.Seq.d/                        755   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433417) Dictyostelium discoideum cDNA clone:ddv21e21, 3' ...   755   0.0    1
(BJ402800) Dictyostelium discoideum cDNA clone:dds18f12, 3' ...   755   0.0    1
(BJ402668) Dictyostelium discoideum cDNA clone:dds17k18, 3' ...   755   0.0    1
(BJ402466) Dictyostelium discoideum cDNA clone:dds17d04, 3' ...   755   0.0    1
(BJ401255) Dictyostelium discoideum cDNA clone:dds22l13, 3' ...   755   0.0    1
(BJ400632) Dictyostelium discoideum cDNA clone:dds14h06, 3' ...   755   0.0    1
(BJ399993) Dictyostelium discoideum cDNA clone:dds8i11, 3' e...   755   0.0    1
(BJ399959) Dictyostelium discoideum cDNA clone:dds8n04, 3' e...   755   0.0    1
(BJ399059) Dictyostelium discoideum cDNA clone:dds3l20, 3' e...   755   0.0    1
(BJ378089) Dictyostelium discoideum cDNA clone:ddc27i05, 3' ...   755   0.0    1
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   259   6e-68
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   259   6e-68
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   259   6e-68
B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   257   2e-67
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   257   2e-67
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   244   2e-63
AF517848_1(AF517848|pid:none) Griffithsia japonica isolate Gj526...   244   2e-63
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   244   2e-63
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   244   2e-63
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   244   2e-63
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM417 is

5' end seq. ID AFM417F
5' end seq. 
>AFM417F.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAAANTTAAAAANTCNNCTTTTTTGAAAAAAAAAA
XXXXXXXXXX
Length of 5' end seq. 130
3' end seq. ID AFM417Z
3' end seq. 
>AFM417Z.Seq
XXXXXXXXXXGNNNNNNNNNNNNNTGGTANNNNNTNNNTNTTNNANTTNAGGNTNNNNTA
ATATTNNNNNTGTTNNNNNNAANATTNAANNNAAANAAGGTATNNCNNCANNNCANNAAA
NATTNATTTTNNNTGGTNNNCAATTANAANATGGTNGTNCTNTNTNTGATTNCAACATTC
AAAAGGAATNCNCTNTCCATTTAGTCNTCAGATTAAGAGGTGGTATGCAAATTTTTGTCA
AAACCCTCACTGGTAAGACTATTACACTNGAAGTTGAAGGTTCTGATAACATTGAGAATG
TTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCG
CCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCA
CTNTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTG
GTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAA
TTCAAGACAAAGAAGGTATTCCA
Length of 3' end seq. 563
Connected seq. ID AFM417P
Connected seq. 
>AFM417P.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAAANTTAAAAANTCNNCTTTTTTGAAAAAAAAAA
----------GNNNNNNNNNNNNNTGGTANNNNNTNNNTNTTNNANTTNAGGNTNNNNTA
ATATTNNNNNTGTTNNNNNNAANATTNAANNNAAANAAGGTATNNCNNCANNNCANNAAA
NATTNATTTTNNNTGGTNNNCAATTANAANATGGTNGTNCTNTNTNTGATTNCAACATTC
AAAAGGAATNCNCTNTCCATTTAGTCNTCAGATTAAGAGGTGGTATGCAAATTTTTGTCA
AAACCCTCACTGGTAAGACTATTACACTNGAAGTTGAAGGTTCTGATAACATTGAGAATG
TTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCG
CCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCA
CTNTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTG
GTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAA
TTCAAGACAAAGAAGGTATTCCA
Length of connected seq. 673
Full length Seq ID -
Full length Seq. -
Length of full length seq. -