>VFG826 (VFG826Q) /CSM/VF/VFG8-B/VFG826Q.Seq.d/
XXXXXXXXXXTCNTTACNCNCTCCNTTGAATTNTCNAACTAAANATATGGNAAACTNTGA
ATCCNTNGAAAAAATNAAAGGNGGNGTTATGATTANCAACGTTTCAAAAGGNGCNTTAGT
TAANGCTTNTGANGCNANTGTTGGTTTAAAATCTGGNAAAANCNGNTCCCTTGGNANGGA
NGNCTATGAAAATGAAACCNATTACTTTTANCAANATCCCNATGGNTCNATCNTTAAANA
TGANAATCNCNCTTTATTAATCTCTTAACCNAATGTTATGATCCCTTCCCNTCAAGCATG
GNACACTAAANAAGCCCTTTCTTGGANGCTGNGGGACNTCNCNTCAAAATTTCGTTGATT
TTAGGNCNAATCCAATTAAAAAANCNAANTTAGTTAANAATCCNATTTCNTCCCNNCCCA
CCCNATNAANTAAAAAAAAAAA

---LXXPLNXXTKXMXNXESXEKXKGGVMIXNVSKGALVXAXXAXVGLKSGKXXSLGXXX
YENETXYFXQXPXGSIXKXXNXXLLIS*pnvmipsxqawxtkxalswxlxdxxskfr*f*
xxsn*kxxxs*xsxfxpxhpxx*kkk

Frame A:
---xyxlx*ixxlxiwxtxnpxkkxkxxl*lxtfqkxx*lxlxxxxlv*nlxkxxplxxx
xmkmkpitfxxipmxxslxmxixly*slnxml*slpxkhgtlxkpflgxxgtsxqnfvdf
rxnpikkxxlvxnpissxptxxxkkk


Frame B:
---xtxsxexsn*xygkl*ixxknxrxxydxqrfkrxxs*xx*xxcwfkiwxxxxpwxgx
l*k*nxlllxxsxwxxx*x*xsxfinlltxcydpfpssmxh*xspflxaxgxxxkislil
gxiqlkxxx*lxixfxpxppxxxkkk


Frame C:
---LXXPLNXXTKXMXNXESXEKXKGGVMIXNVSKGALVXAXXAXVGLKSGKXXSLGXXX
YENETXYFXQXPXGSIXKXXNXXLLIS*pnvmipsxqawxtkxalswxlxdxxskfr*f*
xxsn*kxxxs*xsxfxpxhpxx*kkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG826 (VFG826Q) /CSM/VF/VFG8-B/VFG826Q.Seq.d/                        462   e-129
VFG394 (VFG394Q) /CSM/VF/VFG3-D/VFG394Q.Seq.d/                        377   e-103
VFH856 (VFH856Q) /CSM/VF/VFH8-C/VFH856Q.Seq.d/                        363   1e-99
VFF779 (VFF779Q) /CSM/VF/VFF7-D/VFF779Q.Seq.d/                        353   1e-96
SFE418 (SFE418Q) /CSM/SF/SFE4-A/SFE418Q.Seq.d/                        349   2e-95
AFO359 (AFO359Q) /CSM/AF/AFO3-C/AFO359Q.Seq.d/                        349   2e-95
VFE164 (VFE164Q) /CSM/VF/VFE1-C/VFE164Q.Seq.d/                        345   3e-94
VFL542 (VFL542Q) /CSM/VF/VFL5-B/VFL542Q.Seq.d/                        341   5e-93
VFG876 (VFG876Q) /CSM/VF/VFG8-D/VFG876Q.Seq.d/                        337   8e-92
VSK319 (VSK319Q) /CSM/VS/VSK3-A/VSK319Q.Seq.d/                        333   1e-90

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 2,158,582
Number of Sequences: 32800594
Number of extensions: 2158582
Number of successful extensions: 145149
Number of sequences better than 10.0: 0
length of query: 442
length of database: V,023,486,437
effective HSP length: 22
effective length of query: 420
effective length of database: U,301,873,369
effective search space: 15666786814980
effective search space used: 15666786814980
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 21 (42.1 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UF7) RecName: Full=Putative D-lactate dehydrogenase;        ...   117   1e-33
CP000117_2647(CP000117|pid:none) Anabaena variabilis ATCC 29413,...    71   1e-11
AB1814(AB1814) D-lactate dehydrogenase [imported] - Nostoc sp. (...    71   1e-11
CP001037_2271(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...    70   3e-11
CP000450_1261(CP000450|pid:none) Nitrosomonas eutropha C91, comp...    69   4e-11
CP000749_2997(CP000749|pid:none) Marinomonas sp. MWYL1, complete...    68   9e-11
AY313608_1(AY313608|pid:none) Mastigamoeba balamuthi putative D-...    67   1e-10
CP000250_2250(CP000250|pid:none) Rhodopseudomonas palustris HaA2...    66   4e-10
AP006841_1461(AP006841|pid:none) Bacteroides fragilis YCH46 DNA,...    65   9e-10
CP000885_1901(CP000885|pid:none) Clostridium phytofermentans ISD...    64   1e-09

 psg: 0.66  gvh: 0.43  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFG826 is cyt

>VFG826Z.Seq
----------TCNTTACNCNCTCCNTTGAATTNTCNAACTAAANATATGGNAAACTNTGA
ATCCNTNGAAAAAATNAAAGGNGGNGTTATGATTANCAACGTTTCAAAAGGNGCNTTAGT
TAANGCTTNTGANGCNANTGTTGGTTTAAAATCTGGNAAAANCNGNTCCCTTGGNANGGA
NGNCTATGAAAATGAAACCNATTACTTTTANCAANATCCCNATGGNTCNATCNTTAAANA
TGANAATCNCNCTTTATTAATCTCTTAACCNAATGTTATGATCCCTTCCCNTCAAGCATG
GNACACTAAANAAGCCCTTTCTTGGANGCTGNGGGACNTCNCNTCAAAATTTCGTTGATT
TTAGGNCNAATCCAATTAAAAAANCNAANTTAGTTAANAATCCNATTTCNTCCCNNCCCA
CCCNATNAANTAAAAAAAAAAA