>AFB514 (AFB514Q) /CSM/AF/AFB5-A/AFB514Q.Seq.d/
XXXXXXXXXXTAGCTACCTCCAACAACCAGCTCATGATACCATCATGAGAATGAAACTCT
ATGCTGACTCTTTAGCTCGTTACAGTAAATCACCCATACATTTATCCATTATATGGTTTA
GGTGAATTACCACAAGCATTTGCCCGTTTATCTGCTATCTATGGTGGTACATATATGCTT
AATAAACCAATTGAAAATATTGTTTTCGATGAAGAAAAGAAAATTATTAAAGTTACATCT
GAAGGTGAGACTGTAACATGTGATAAAATTGTTTGTGATCCATCATATTTCCCAAATAAA
GTTCAAAAGAAAGGTAAAATTATTCGTTCAATTTGTATTTTGAATGGTCCAATTCCATCT
GCTGATAAGAAATTTGAATCACTCCAAATCATTATCCCACAAAAACAAGTTAAACGTCAA
TCAGATATGTACGTTGGTGTTATGTCATCCGCTCATTGTGTCTGTCCAAAGGGTAAATAC
ATTGCCATCGTTTCAGCCACTGTTGAAACCAATGAACCAGAGAAAGAATTAGCACCAGCT
TACGCTTTATTAGGTCCAATTCAAGAGAAATTTGTCTATATTTCTGATTTCATTGAACCA
ATTGCTGATGGTACTGCCGACAATGTTTTCATCTCAAAATCATATGATGCTACCTCTCAC
TTTGAAACCACTTGTGAAGATGTCATGGATATGTACAAGAGAATCACGGTAAAGATTTAG
TTCTCACTGTTCATCCAAAATTCTTAACAACAAGAAGAAGCGGAACCAAGAACAAGAAGA
ACA

---atsnnqlmips*e*nsmltl*LVTVNHPYIYPLYGLGELPQAFARLSAIYGGTYMLN
KPIENIVFDEEKKIIKVTSEGETVTCDKIVCDPSYFPNKVQKKGKIIRSICILNGPIPSA
DKKFESLQIIIPQKQVKRQSDMYVGVMSSAHCVCPKGKYIAIVSATVETNEPEKELAPAY
ALLGPIQEKFVYISDFIEPIADGTADNVFISKSYDATSHFETTCEDVMDMYKRITVKI*f
slfiqns*qqeeaeprtrrt

Frame A:
---sylqqpahdtimrmklyadslaryskspihlsiiwfr*ittsicpficylwwyiya*
*tn*kycfr*rkeny*syi*r*dcnm**ncl*siifpk*ssker*nysfnlyfewsnsic
**ei*itpnhyptkts*tsiryvrwcyvirslclskg*ihchrfshc*nq*treristsl
rfirsnsreiclyf*fh*tnc*wycrqcfhlkii*cylsl*nhl*rchgyvqenhgkdlv
ltvhpkflttrrsgtknkkn


Frame B:
---atsnnqlmips*e*nsmltl*LVTVNHPYIYPLYGLGELPQAFARLSAIYGGTYMLN
KPIENIVFDEEKKIIKVTSEGETVTCDKIVCDPSYFPNKVQKKGKIIRSICILNGPIPSA
DKKFESLQIIIPQKQVKRQSDMYVGVMSSAHCVCPKGKYIAIVSATVETNEPEKELAPAY
ALLGPIQEKFVYISDFIEPIADGTADNVFISKSYDATSHFETTCEDVMDMYKRITVKI*f
slfiqns*qqeeaeprtrrt


Frame C:
---lppttss*yhhenetlc*lfsslq*ithtfihymv*vnyhkhlpvyllsmvvhicli
nqlkilfsmkkrkllklhlkvrl*hviklfvihhisqikfkrkvklfvqfvf*mvqfhll
irnlnhskslshknklnvnqictlvlchplivsvqrvntlpsfqpllkpmnqrkn*hqlt
ly*vqfkrnlsiflislnqllmvlptmfssqnhmmlpltlkplvkmswictresr*rfss
hcsskilnnkkkrnqeqee

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB514 (AFB514Q) /CSM/AF/AFB5-A/AFB514Q.Seq.d/                       1532   0.0
CFH515 (CFH515Q) /CSM/CF/CFH5-A/CFH515Q.Seq.d/                       1445   0.0
SFL479 (SFL479Q) /CSM/SF/SFL4-D/SFL479Q.Seq.d/                       1441   0.0
AFE889 (AFE889Q) /CSM/AF/AFE8-D/AFE889Q.Seq.d/                       1423   0.0
SFD219 (SFD219Q) /CSM/SF/SFD2-A/SFD219Q.Seq.d/                       1405   0.0
CFH888 (CFH888Q) /CSM/CF/CFH8-D/CFH888Q.Seq.d/                       1402   0.0
AFN874 (AFN874Q) /CSM/AF/AFN8-D/AFN874Q.Seq.d/                       1400   0.0
SFL120 (SFL120Q) /CSM/SF/SFL1-A/SFL120Q.Seq.d/                       1394   0.0
AFD453 (AFD453Q) /CSM/AF/AFD4-C/AFD453Q.Seq.d/                       1392   0.0
VFJ639 (VFJ639Q) /CSM/VF/VFJ6-B/VFJ639Q.Seq.d/                       1388   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ005836|AJ005836.1 Cicer arietinum mRNA for GDP dissociation inhibitor (gdi).    38  4e-08  4
BZ296562|BZ296562.1 CG2922.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG2922, DNA sequence.    66  7e-07  1
AL408803|AL408803.1 T3 end of clone AV0AA010G06 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    40  5e-06  3
BX567109|BX567109.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse86b08_q1c.    62  1e-05  1
BI315670|BI315670.1 saf75f12.y1 Gm-c1078 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1078-2184 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    54  1e-05  2
AI437629|AI437629.1 sa37e09.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-1505 5' similar to TR:O24653 O24653 GDI2. ;, mRNA sequence.    54  1e-05  2
BE382073|BE382073.1 601272233F1 NCI_CGAP_Mam1 Mus musculus cDNA clone IMAGE:3601424 5', mRNA sequence.    34  4e-05  4
AI724375|AI724375.1 RHIZ1_34_D10.y2_A001 Rhizome1 (RHIZ1) Sorghum halepense cDNA, mRNA sequence.    60  4e-05  1
CD444569|CD444569.1 EL01N0441D10.b Endosperm_4 Zea mays cDNA, mRNA sequence.    60  4e-05  1
AI783359|AI783359.1 614010H03.x2 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence.    60  4e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF251042_1(AF251042|pid:none) Mus musculus rab GDP dissociation ...   257   8e-73
(O97555) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   255   2e-72
(P50398) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   255   2e-72
AF130987_1(AF130987|pid:none) Rattus norvegicus RAB GDP dissocia...   255   2e-72
X74402_1(X74402|pid:none) R.norvegicus rab GDI alpha mRNA.            255   4e-72
(P21856) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   254   4e-72
(Q8HXX7) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   254   5e-72
CR926033_1(CR926033|pid:none) Pongo abelii mRNA; cDNA DKFZp459G1...   254   5e-72
BC095329_1(BC095329|pid:none) Danio rerio GDP dissociation inhib...   253   5e-72
AY665236_1(AY665236|pid:none) Saimiri boliviensis GDP dissociati...   254   5e-72

 psg: 0.85  gvh: 0.62  alm: 0.47  top: 0.43  tms: 0.00  mit: 0.21  mip: 0.05
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: extracellular, including cell wall
	  8.0 %: cytoskeletal
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFB514 is cyt

>AFB514Z.Seq
----------TAGCTACCTCCAACAACCAGCTCATGATACCATCATGAGAATGAAACTCT
ATGCTGACTCTTTAGCTCGTTACAGTAAATCACCCATACATTTATCCATTATATGGTTTA
GGTGAATTACCACAAGCATTTGCCCGTTTATCTGCTATCTATGGTGGTACATATATGCTT
AATAAACCAATTGAAAATATTGTTTTCGATGAAGAAAAGAAAATTATTAAAGTTACATCT
GAAGGTGAGACTGTAACATGTGATAAAATTGTTTGTGATCCATCATATTTCCCAAATAAA
GTTCAAAAGAAAGGTAAAATTATTCGTTCAATTTGTATTTTGAATGGTCCAATTCCATCT
GCTGATAAGAAATTTGAATCACTCCAAATCATTATCCCACAAAAACAAGTTAAACGTCAA
TCAGATATGTACGTTGGTGTTATGTCATCCGCTCATTGTGTCTGTCCAAAGGGTAAATAC
ATTGCCATCGTTTCAGCCACTGTTGAAACCAATGAACCAGAGAAAGAATTAGCACCAGCT
TACGCTTTATTAGGTCCAATTCAAGAGAAATTTGTCTATATTTCTGATTTCATTGAACCA
ATTGCTGATGGTACTGCCGACAATGTTTTCATCTCAAAATCATATGATGCTACCTCTCAC
TTTGAAACCACTTGTGAAGATGTCATGGATATGTACAAGAGAATCACGGTAAAGATTTAG
TTCTCACTGTTCATCCAAAATTCTTAACAACAAGAAGAAGCGGAACCAAGAACAAGAAGA
ACA