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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM791
Library AF
(Link to library)
Clone ID AFM791
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM791P
(Link to Original site)
Representative DNA sequence 
>AFM791 (AFM791Q) /CSM/AF/AFM7-D/AFM791Q.Seq.d/
ATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTG
GTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCG
CTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTG
ATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTT
TAAGAACTGCTTGTGAACGTGCCAAAAGAACTCTTTCATCATCTGCTCAAGCTTCAATTG
AAATTGATTCACTCTTTGAAGGTATTGATTTCTATACTTCAATTACTAGAGCACGTTTTG
AAGAACTCTGTGCTGATCTTTTCCGTGGTTGTTTAGATCCAGTTGAAAAAGTATTAAAAG
ATAGTAAATTGGATAAGAAATCAATTCATGAAATTGTTTTAGTTGGTGGTTCAACTCGTA
TTCCAAAGGTACAACAATTATTACAAGAATTTTTCAATGGTAAAGAATTGAATAAATCAA
TTAATCCAGATGAAGCTGTTGCTTATGGTGCCCGCTGTACAAGCTGCAATTCTTTCAXXX
XXXXXXXGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGAC
CAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGG
TGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGC
TGAAGATAAATCAACTGGTAACAAACAAAAGACTACAATTACCAATGATAAAGGTCGTCT
TTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGA
ACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAA
AAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTAT
TGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGA
TGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACT
CTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTC
ACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAA
CATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTC
ATATA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
GLEKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVD
EFKRKHKKDIMGNQRAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFE
ELCADLFRGCLDPVEKVLKDSKLDKKSIHEIVLVGGSTRIPKVQQLLQEFFNGKELNKSI
NPDEAVAYGARCTSCNSF---

---DNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVS
AEDKSTGNKQKTTITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sf
sy
Translated Amino Acid sequence (All Frames) 
Frame A:
mv*krkvlvkrif*fsi*vvvhlmflslplkmvylklkpplvili*vvkiliivsfltll
mnlnvnikkilwvinvlfvv*ellvnvpkelfhhllklqlklihslkvlisilqllehvl
knsvlifsvvv*iqlkky*kivnwirnqfmklf*lvvqlvfqrynnyyknfsmvkn*inq
liqmklllmvpavqaails---

---DNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVS
AEDKSTGNKQKTTITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sf
sy

Frame B:
wfrkerfw*keyfnfrfrwwyi*cfspyh*rwci*s*shrw*ysfrw*rf**ssrfslc*
*i*t*t*kryyg*stccssfkncl*tcqknsfiicssfn*n*ftl*ry*flyfny*stf*
rtlc*sfpwlfrss*ksikr**ig*eins*ncfswwfnsyskgttiitrifqw*rie*in
*sr*scclwcplyklqff---

---iinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myq
lkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsll
kiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vn
sikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vskinkas
hi

Frame C:
GLEKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVD
EFKRKHKKDIMGNQRAVRRLRTACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFE
ELCADLFRGCLDPVEKVLKDSKLDKKSIHEIVLVGGSTRIPKVQQLLQEFFNGKELNKSI
NPDEAVAYGARCTSCNSF---

---*strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkcis
*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*
kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*t
lsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksikll
i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM791 (AFM791Q) /CSM/AF/AFM7-D/AFM791Q.Seq.d/                       2567   0.0
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1415   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1415   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1415   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1415   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1415   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1415   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1415   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1415   0.0
VHP111 (VHP111Q) /CSM/VH/VHP1-A/VHP111Q.Seq.d/                       1415   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347338) Dictyostelium discoideum cDNA clone:dda26f23, 3' ...  1421   0.0    1
(X75263) D.discoideum (AX3) mRNA for heat shock cognate prot...  1413   0.0    1
(BJ446704) Dictyostelium discoideum cDNA clone:ddv63g16, 3' ...  1413   0.0    1
(BJ446275) Dictyostelium discoideum cDNA clone:ddv62p03, 3' ...  1413   0.0    1
(BJ446228) Dictyostelium discoideum cDNA clone:ddv62f05, 3' ...  1413   0.0    1
(BJ446038) Dictyostelium discoideum cDNA clone:ddv61o08, 3' ...  1413   0.0    1
(BJ446016) Dictyostelium discoideum cDNA clone:ddv61j10, 3' ...  1413   0.0    1
(BJ446000) Dictyostelium discoideum cDNA clone:ddv61g10, 3' ...  1413   0.0    1
(BJ445963) Dictyostelium discoideum cDNA clone:ddv61o06, 3' ...  1413   0.0    1
(BJ445929) Dictyostelium discoideum cDNA clone:ddv61h06, 3' ...  1413   0.0    1
dna update 2009. 4.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB170103_1(AB170103|pid:none) Macaca fascicularis brain cDNA clo...   246   e-130
EU684308_1(EU684308|pid:none) Exorista civilis heat shock protei...   275   e-125
(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   355   2e-96
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   355   2e-96
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   330   5e-89
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   327   6e-88
BC121673_1(BC121673|pid:none) Xenopus tropicalis heat shock 70kD...   313   1e-83
(P17879) RecName: Full=Heat shock 70 kDa protein 1B; AltName: Fu...   311   3e-83
AK171820_1(AK171820|pid:none) Mus musculus activated spleen cDNA...   311   3e-83
(Q61696) RecName: Full=Heat shock 70 kDa protein 1A; AltName: Fu...   311   3e-83
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM791 is

5' end seq. ID AFM791F
5' end seq. 
>AFM791F.Seq
ATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTG
GTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCG
CTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTG
ATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTT
TAAGAACTGCTTGTGAACGTGCCAAAAGAACTCTTTCATCATCTGCTCAAGCTTCAATTG
AAATTGATTCACTCTTTGAAGGTATTGATTTCTATACTTCAATTACTAGAGCACGTTTTG
AAGAACTCTGTGCTGATCTTTTCCGTGGTTGTTTAGATCCAGTTGAAAAAGTATTAAAAG
ATAGTAAATTGGATAAGAAATCAATTCATGAAATTGTTTTAGTTGGTGGTTCAACTCGTA
TTCCAAAGGTACAACAATTATTACAAGAATTTTTCAATGGTAAAGAATTGAATAAATCAA
TTAATCCAGATGAAGCTGTTGCTTATGGTGCCCGCTGTACAAGCTGCAATTCTTTCAXXX
XXXXXXX
Length of 5' end seq. 607
3' end seq. ID AFM791Z
3' end seq. 
>AFM791Z.Seq
XXXXXXXXXXGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTAT
GACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAG
AGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATC
AGCTGAAGATAAATCAACTGGTAACAAACAAAAGACTACAATTACCAATGATAAAGGTCG
TCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGA
TGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGT
TAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAAC
TATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAA
GGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAA
ACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGA
TTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGA
AAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTT
CTCATATA
Length of 3' end seq. 728
Connected seq. ID AFM791P
Connected seq. 
>AFM791P.Seq
ATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTG
GTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCG
CTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTG
ATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTT
TAAGAACTGCTTGTGAACGTGCCAAAAGAACTCTTTCATCATCTGCTCAAGCTTCAATTG
AAATTGATTCACTCTTTGAAGGTATTGATTTCTATACTTCAATTACTAGAGCACGTTTTG
AAGAACTCTGTGCTGATCTTTTCCGTGGTTGTTTAGATCCAGTTGAAAAAGTATTAAAAG
ATAGTAAATTGGATAAGAAATCAATTCATGAAATTGTTTTAGTTGGTGGTTCAACTCGTA
TTCCAAAGGTACAACAATTATTACAAGAATTTTTCAATGGTAAAGAATTGAATAAATCAA
TTAATCCAGATGAAGCTGTTGCTTATGGTGCCCGCTGTACAAGCTGCAATTCTTTCA---
-------GATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGAC
CAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGG
TGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGC
TGAAGATAAATCAACTGGTAACAAACAAAAGACTACAATTACCAATGATAAAGGTCGTCT
TTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGA
ACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAA
AAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTAT
TGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGA
TGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACT
CTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTC
ACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAA
CATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTC
ATATA
Length of connected seq. 1315
Full length Seq ID -
Full length Seq. -
Length of full length seq. -