>VHA135 (VHA135Q) /CSM/VH/VHA1-B/VHA135Q.Seq.d/
ATTATTTTATTTTATAAGCTTTGAAGAAAATGAGAGAATTTTTAAAAGATGATTATGATG
TAATAGTTTGTGGTGGTGGTCCAGTTGGTTTGGCTACTGCATACAGATGTGCCAAAGCTG
GAAAGAAAGTACTTTGTCTCGAAAAATCAGTATTCTTTAATGGAGGTGGTAGTAGTGGTG
ATGTTGTTAGAATGTTACGTACAATGTACACTGAAGATTATATGGCCGATTTAGCACATG
AAACCCTTGGACTCTGGAAAGAACTTGGTGATGATGCTGGTGAAGGTGATTTAGTTTGGA
TGACAGGTCTCCTAAATTTTGGTGATCCAAATTATGGTGCAGGTGGTCCAGAAGGTACTT
TATTAGGACCAATTCCAAATTTAGAGAGATTGGGTATGCAATACAAAGTGTTAACTGCTC
AAGAAATTATGGAAGAGTATCCATTTAGAAACATTCCATCCAATCACCAAGGTGTTTTTG
CTCCAGACAATGGTGTTATCAATTTACCATTGGTATTACGTTCTCTTTACAAGTTATGTT
TACAATATGGTTGTAAAATGGTAAGCCAXXXXXXXXXXGGTACCCTCCAACAATTATTTG
TTGATTACAAANGTTGGGATTGGNANAANGATGATTATTNATCAGGGGCATTTGNTTTTT
ACAGTCCATCNCAATTTTTTCAATTGTTCCCATCNCTTANTAAACCATCNCCANATGGTA
NATTCCNTTTAGNTGGNGAANCAACATNTGTTNATCANGGNTGGGTCANTGGTTTTTTTA
ANTTTGCCTATNGNTCAGTNGATCNCATTTTTCAAGTTGAAGGTTTTGATGAGTTACGNG
CCAAANTANGTTTCAANTGGGGNTTNATCGANGAAGTTGAANATCCTCAANATGATCAAC
AATATGTTGACCCNCATCNCAATTTAAGTG

yfil*ALKKMREFLKDDYDVIVCGGGPVGLATAYRCAKAGKKVLCLEKSVFFNGGGSSGD
VVRMLRTMYTEDYMADLAHETLGLWKELGDDAGEGDLVWMTGLLNFGDPNYGAGGPEGTL
LGPIPNLERLGMQYKVLTAQEIMEEYPFRNIPSNHQGVFAPDNGVINLPLVLRSLYKLCL
QYGCKMVS---

---GTLQQLFVDYKXWDWXXDDYXSGAFXFYSPSQFFQLFPSLXKPSPXGXFXLXGEXTX
VXXGWVXGFFXFAYXSVDXIFQVEGFDELRAKXXFXWGXIXEVEXPQXDQQYVDPHXNLS

Frame A:
iilfykl*rk*enf*kmimm**fvvvvqlvwllhtdvpklerkyfvsknqyslmevvvvv
mllecyvqctlkiiwpi*hmkpldsgknlvmmlvkvi*fg*qvs*ilviqimvqvvqkvl
y*dqfqi*rdwvcntkc*llkklwksihletfhpitkvfllqtmvlsiyhwyyvlftsyv
ynmvvkw*a---

---GTLQQLFVDYKXWDWXXDDYXSGAFXFYSPSQFFQLFPSLXKPSPXGXFXLXGEXTX
VXXGWVXGFFXFAYXSVDXIFQVEGFDELRAKXXFXWGXIXEVEXPQXDQQYVDPHXNLS

Frame B:
lfyfisfeenerifkr*l*cnslwwwsswfgyciqmcqswkestlsrkisil*wrw**w*
cc*nvtynvh*rlygrfst*npwtlertw**cw*r*fslddrspkfw*sklwcrwsrryf
irtnskfreigyaiqsvncsrnygrvsi*khsiqsprcfcsrqwcyqftigitfslqvmf
tiwl*ngkp---

---vpsnnyllitxvgigxxmiixqghlxftvhxnffncshxlxnhxxmvxsx*xxxqhx
xixxgsxvflxlpxxqxixffklkvlmsyxpxxvsxgxxsxklxilxminnmltxixi*v

Frame C:
yfil*ALKKMREFLKDDYDVIVCGGGPVGLATAYRCAKAGKKVLCLEKSVFFNGGGSSGD
VVRMLRTMYTEDYMADLAHETLGLWKELGDDAGEGDLVWMTGLLNFGDPNYGAGGPEGTL
LGPIPNLERLGMQYKVLTAQEIMEEYPFRNIPSNHQGVFAPDNGVINLPLVLRSLYKLCL
QYGCKMVS---

---ypptiic*lqxlglxxx*lxirgixflqsixifsivpixx*tixxwxipfxwxxnxc
xsxxgxwff*xclxxsxshfss*rf**vtxqxxfqxgxxrxs*xssx*stic*pxsqfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA135 (VHA135Q) /CSM/VH/VHA1-B/VHA135Q.Seq.d/                       1522   0.0
VHQ418 (VHQ418Q) /CSM/VH/VHQ4-A/VHQ418Q.Seq.d/                       1126   0.0
VHL890 (VHL890Q) /CSM/VH/VHL8-D/VHL890Q.Seq.d/                       1126   0.0
VHK762 (VHK762Q) /CSM/VH/VHK7-C/VHK762Q.Seq.d/                       1126   0.0
VHK414 (VHK414Q) /CSM/VH/VHK4-A/VHK414Q.Seq.d/                       1126   0.0
VHI858 (VHI858Q) /CSM/VH/VHI8-C/VHI858Q.Seq.d/                       1126   0.0
VHD838 (VHD838Q) /CSM/VH/VHD8-B/VHD838Q.Seq.d/                       1126   0.0
VHB454 (VHB454Q) /CSM/VH/VHB4-C/VHB454Q.Seq.d/                       1126   0.0
VHB252 (VHB252Q) /CSM/VH/VHB2-C/VHB252Q.Seq.d/                       1126   0.0
SHL131 (SHL131Q) /CSM/SH/SHL1-B/SHL131Q.Seq.d/                       1126   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC009598|AC009598.3 Homo sapiens chromosome 8 clone RP11-223O12 map 8, WORKING DRAFTSEQUENCE, 9 unordered pieces.    40   0.046  4
CQ344998|CQ344998.1 Sequence 19092 from Patent WO0157275.          38   0.29   2
CQ308193|CQ308193.1 Sequence 19298 from Patent WO0186003.          38   0.29   2
CQ270885|CQ270885.1 Sequence 19146 from Patent WO0157277.          38   0.29   2
CQ232880|CQ232880.1 Sequence 19719 from Patent WO0157273.          38   0.29   2
CQ184373|CQ184373.1 Sequence 15769 from Patent WO0157274.          38   0.29   2
CQ149594|CQ149594.1 Sequence 19616 from Patent WO0157276.          38   0.29   2
CQ110849|CQ110849.1 Sequence 19708 from Patent WO0157272.          38   0.29   2
EJ261209|EJ261209.1 1095349040450 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061005495618 3', genomic survey sequence.    48   0.43   1
EJ150489|EJ150489.1 1092343756363 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001453166 5', genomic survey sequence.    48   0.43   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CU638744_413(CU638744|pid:none) Podospora anserina genomic DNA c...   204   4e-51
Y18574_4(Y18574|pid:none) Streptomyces tendae strain Tue901, nik...   109   1e-22
AM286415_1545(AM286415|pid:none) Yersinia enterocolitica subsp. ...    80   1e-13
CP001291_5186(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...    78   5e-13
(Q29RU9) RecName: Full=Peroxisomal sarcosine oxidase;          S...    77   8e-13
AY892312_1(AY892312|pid:none) Synthetic construct Homo sapiens c...    76   1e-12
CR457155_1(CR457155|pid:none) Homo sapiens full open reading fra...    76   1e-12
AF134593_1(AF134593|pid:none) Homo sapiens L-pipecolic acid oxid...    76   1e-12
AX882278_1(AX882278|pid:none) Sequence 17183 from Patent EP10746...    76   1e-12
BC114006_1(BC114006|pid:none) Bos taurus L-pipecolic acid oxidas...    76   2e-12

 psg: 0.67  gvh: 0.49  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for VHA135 is cyt

>VHA135F.Seq
ATTATTTTATTTTATAAGCTTTGAAGAAAATGAGAGAATTTTTAAAAGATGATTATGATG
TAATAGTTTGTGGTGGTGGTCCAGTTGGTTTGGCTACTGCATACAGATGTGCCAAAGCTG
GAAAGAAAGTACTTTGTCTCGAAAAATCAGTATTCTTTAATGGAGGTGGTAGTAGTGGTG
ATGTTGTTAGAATGTTACGTACAATGTACACTGAAGATTATATGGCCGATTTAGCACATG
AAACCCTTGGACTCTGGAAAGAACTTGGTGATGATGCTGGTGAAGGTGATTTAGTTTGGA
TGACAGGTCTCCTAAATTTTGGTGATCCAAATTATGGTGCAGGTGGTCCAGAAGGTACTT
TATTAGGACCAATTCCAAATTTAGAGAGATTGGGTATGCAATACAAAGTGTTAACTGCTC
AAGAAATTATGGAAGAGTATCCATTTAGAAACATTCCATCCAATCACCAAGGTGTTTTTG
CTCCAGACAATGGTGTTATCAATTTACCATTGGTATTACGTTCTCTTTACAAGTTATGTT
TACAATATGGTTGTAAAATGGTAAGCCANNNNNNNNNN

>VHA135Z.Seq
NNNNNNNNNNGGTACCCTCCAACAATTATTTGTTGATTACAAANGTTGGGATTGGNANAA
NGATGATTATTNATCAGGGGCATTTGNTTTTTACAGTCCATCNCAATTTTTTCAATTGTT
CCCATCNCTTANTAAACCATCNCCANATGGTANATTCCNTTTAGNTGGNGAANCAACATN
TGTTNATCANGGNTGGGTCANTGGTTTTTTTAANTTTGCCTATNGNTCAGTNGATCNCAT
TTTTCAAGTTGAAGGTTTTGATGAGTTACGNGCCAAANTANGTTTCAANTGGGGNTTNAT
CGANGAAGTTGAANATCCTCAANATGATCAACAATATGTTGACCCNCATCNCAATTTAAG
TG

>VHA135P.Seq
ATTATTTTATTTTATAAGCTTTGAAGAAAATGAGAGAATTTTTAAAAGATGATTATGATG
TAATAGTTTGTGGTGGTGGTCCAGTTGGTTTGGCTACTGCATACAGATGTGCCAAAGCTG
GAAAGAAAGTACTTTGTCTCGAAAAATCAGTATTCTTTAATGGAGGTGGTAGTAGTGGTG
ATGTTGTTAGAATGTTACGTACAATGTACACTGAAGATTATATGGCCGATTTAGCACATG
AAACCCTTGGACTCTGGAAAGAACTTGGTGATGATGCTGGTGAAGGTGATTTAGTTTGGA
TGACAGGTCTCCTAAATTTTGGTGATCCAAATTATGGTGCAGGTGGTCCAGAAGGTACTT
TATTAGGACCAATTCCAAATTTAGAGAGATTGGGTATGCAATACAAAGTGTTAACTGCTC
AAGAAATTATGGAAGAGTATCCATTTAGAAACATTCCATCCAATCACCAAGGTGTTTTTG
CTCCAGACAATGGTGTTATCAATTTACCATTGGTATTACGTTCTCTTTACAAGTTATGTT
TACAATATGGTTGTAAAATGGTAAGCCA----------GGTACCCTCCAACAATTATTTG
TTGATTACAAANGTTGGGATTGGNANAANGATGATTATTNATCAGGGGCATTTGNTTTTT
ACAGTCCATCNCAATTTTTTCAATTGTTCCCATCNCTTANTAAACCATCNCCANATGGTA
NATTCCNTTTAGNTGGNGAANCAACATNTGTTNATCANGGNTGGGTCANTGGTTTTTTTA
ANTTTGCCTATNGNTCAGTNGATCNCATTTTTCAAGTTGAAGGTTTTGATGAGTTACGNG
CCAAANTANGTTTCAANTGGGGNTTNATCGANGAAGTTGAANATCCTCAANATGATCAAC
AATATGTTGACCCNCATCNCAATTTAAGTG