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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB359
Library VH
(Link to library)
Clone ID VHB359
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16430-1
Original site URL
Representative seq. ID VHB359P
(Link to Original site)
Representative DNA sequence 
>VHB359 (VHB359Q) /CSM/VH/VHB3-C/VHB359Q.Seq.d/
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAN
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTGGTGAATTTACCATTGCTCTCGATCATACCGAACCATTAGGTCGXXXXXXXXXXTCTC
CATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGTGACCCAATTG
ATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCA
AAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAG
CTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGG
TAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTT
GGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCAT
CATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATT
TAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATG
AAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTA
TTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAG
AATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCAT
CTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCTATCAATTTTTTGGA
ATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAAATCTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
ftfrsyilihn*FYLFFFVXHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLREL
ISNASDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLG
TIARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYVWESSAG
GEFTIALDHTEPLG---

---SPFIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKA
EQDKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRD
SSMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSS
FASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*llalll
sifwnivill**nkf*kkss
Translated Amino Acid sequence (All Frames) 
Frame A:
tshlgpif*fiinficfflxnikkwlnhklnvshsklklislcplllilfiqikkyf*en
*fqmhlmp*ikfvtnh*lmhqfwnqklnsklklfqirlpkllhslivvlv*pkpiw*ri*
vlllvqvlktlwnnykvvplisv*lvnlvlvstllt*lpilllftlktmmmnntfgnqvl
vvnlpllsiipnh*v---

---SPFIEGLKKKNLEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKA
EQDKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRD
SSMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSS
FASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*llalll
sifwnivill**nkf*kkss

Frame B:
lhi*vlysns*lilfvffcxt*kng*its*tfhips*n*sayvpyy*yflfk*rsifkri
dfkci*clr*nslpiin*cisfgikn*trn*nysr*dcqnsyth**wywydqnrygkefr
yycsfry*klygtitkwcr*yqydwsiwcwfllcllscrycycsl*kq***tirlgikcw
w*iyhcsrsyrtirs---

---lhslkvsrrrilksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpkmkrkkl
nkiklpmknysnklkmywviklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*klklsvt
lqchhtchqrkl*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhh
llhvfiv*ln*v*afkmihqqqlknqqtqqpvmifhh*kktmnhlkwkkstrfny*pfcy
qffgillyyynkinfkknlq

Frame C:
ftfrsyilihn*FYLFFFVXHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLREL
ISNASDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLG
TIARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYVWESSAG
GEFTIALDHTEPLG---

---sih*rsqeees*shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr*keks*
tr*scq*ritqts*rcig**s*kgsslnsscqltmcfsyl*iwlvskhgtyhessssp*l
fnviihvikenfriksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiii
cftyssyd*irfkhsr*fisnn*rinkhnnq**ystirrkr*ti*ngksrldlitspfai
nfleycyiiiik*ilkkif
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB359 (VHB359Q) /CSM/VH/VHB3-C/VHB359Q.Seq.d/                       1830   0.0
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                       1019   0.0
VHP148 (VHP148Q) /CSM/VH/VHP1-B/VHP148Q.Seq.d/                       1019   0.0
VHL542 (VHL542Q) /CSM/VH/VHL5-B/VHL542Q.Seq.d/                       1019   0.0
VHK153 (VHK153Q) /CSM/VH/VHK1-C/VHK153Q.Seq.d/                       1019   0.0
VHK102 (VHK102Q) /CSM/VH/VHK1-A/VHK102Q.Seq.d/                       1019   0.0
VHI607 (VHI607Q) /CSM/VH/VHI6-A/VHI607Q.Seq.d/                       1019   0.0
VHH735 (VHH735Q) /CSM/VH/VHH7-B/VHH735Q.Seq.d/                       1019   0.0
VHH731 (VHH731Q) /CSM/VH/VHH7-B/VHH731Q.Seq.d/                       1019   0.0
VHH708 (VHH708Q) /CSM/VH/VHH7-A/VHH708Q.Seq.d/                       1019   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB040814|AB040814.2 Dictyostelium discoideum Dd-grp94 mRNA for glucose-regulated protein 94, complete cds.    78   6e-37  6
AA549925|AA549925.1 0999m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 0999m, DNA sequence.    92   3e-28  4
AB092411|AB092411.1 Entamoeba histolytica gene for cytosolic-type hsp90, partial cds.    58   1e-26  5
AB264546|AB264546.1 Nicotiana tabacum hsp90 mRNA for heat shock protein 90, complete cds.    74   7e-25  4
AB011476|AB011476.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MDA7.    86   7e-24  4
AB060275|AB060275.1 Bombyx mori hsp90 mRNA for 90-kDa heat shock protein, complete cds.    64   7e-22  5
AB025606|AB025606.1 Arabidopsis thaliana genomic DNA, chromosome 5, BAC clone:F6N7.    60   2e-21  6
AB206477|AB206477.1 Chilo suppressalis hsp90 mRNA for heat shock protein 90, complete cds.    92   3e-21  2
AC004688|AC004688.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    56   9e-15  7
AC004709|AC004709.3 Plasmodium falciparum chromosome 12, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    56   2e-14  7
dna update 2007. 4. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             334   5e-90
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   334   5e-90
AB300196_1(AB300196|pid:none) Physarum polycephalum hsp90 mRNA f...   266   1e-69
FJ392027_1(FJ392027|pid:none) Portunus trituberculatus heat shoc...   259   1e-67
(P04810) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   258   3e-67
AY851367_1(AY851367|pid:none) Liriomyza huidobrensis heat shock ...   258   3e-67
U57467_1(U57467|pid:none) Drosophila melanogaster heat shock pro...   258   3e-67
(P04811) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   258   3e-67
U57459_1(U57459|pid:none) Drosophila melanogaster heat shock pro...   258   3e-67
EU306519_1(EU306519|pid:none) Mythimna separata Hsp90 mRNA, comp...   258   5e-67
protein update 2009. 6.27
PSORT 

 psg: 0.50  gvh: 0.46  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB359 is cyt
5' end seq. ID VHB359F
5' end seq. 
>VHB359F.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAN
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTGGTGAATTTACCATTGCTCTCGATCATACCGAACCATTAGGTCGNNNNNNNNNN
Length of 5' end seq. 596
3' end seq. ID VHB359Z
3' end seq. 
>VHB359Z.Seq
NNNNNNNNNNTCTCCATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACAT
GTGTGACCCAATTGATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATT
AGTATCAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGC
TGAACAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGA
TAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTAC
CTCTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGA
CTCTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCC
AATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGT
TTACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATC
ATTTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATC
AGCAACAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGA
AAACGATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCT
ATCAATTTTTTGGAATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAAATCTTC
AA
Length of 3' end seq. 782
Connected seq. ID VHB359P
Connected seq. 
>VHB359P.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAN
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTGGTGAATTTACCATTGCTCTCGATCATACCGAACCATTAGGTCG----------TCTC
CATTCATTGAAGGTCTCAAGAAGAAGAATCTTGAAGTCATTTACATGTGTGACCCAATTG
ATGAATATGCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCA
AAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAG
CTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGG
TAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTT
GGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCAT
CATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATT
TAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATG
AAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTA
TTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAG
AATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCAT
CTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCTATCAATTTTTTGGA
ATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAAATCTTCAA
Length of connected seq. 1358
Full length Seq ID -
Full length Seq. -
Length of full length seq. -