>VHB564 (VHB564Q) /CSM/VH/VHB5-C/VHB564Q.Seq.d/
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AATTXXXXXXXXXX

NSRVQKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
N---

Frame A:
NSRVQKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
N---


Frame B:
tleckkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
---


Frame C:
lssakkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB564 (VHB564Q) /CSM/VH/VHB5-C/VHB564Q.Seq.d/                        680   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             674   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        674   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        674   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             674   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        674   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        674   0.0
VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                        674   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                        674   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                        674   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   672   0.0    1
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   1e-20  3
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   4e-20  3
AR550598|AR550598.1 Sequence 5729 from patent US 6747137.          66   1e-18  3
CO080174|CO080174.1 GR__Ea43H12.r GR__Ea Gossypium raimondii cDNA clone GR__Ea43H12 3', mRNA sequence.    58   4e-18  4
EL513430|EL513430.1 CHUX465.b1_A21.ab1 CHU(XYZ) puzzle sunflower Helianthus paradoxus cDNA clone CHUX465, mRNA sequence.    72   1e-17  4
EL476245|EL476245.1 CHUL4181.b1_J14.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL4181, mRNA sequence.    72   1e-17  4
DY941362|DY941362.1 CHPX9185.b1_B18.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPX9185, mRNA sequence.    72   1e-17  4
DY938228|DY938228.1 CHPX5032.b1_O09.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPX5032, mRNA sequence.    72   1e-17  4
DY907212|DY907212.1 CHAX1940.b1_G05.ab1 CHA(XYZ) common wild sunflower Helianthus annuus cDNA clone CHAX1940, mRNA sequence.    72   2e-17  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   145   3e-34
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   145   4e-34
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   143   2e-33
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   141   6e-33
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   140   2e-32
AY144924_1(AY144924|pid:none) Saccharomyces castellii clone Cont...   139   3e-32
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   139   3e-32
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   139   4e-32
AP005845_16(AP005845|pid:none) Oryza sativa Japonica Group genom...   137   9e-32
(Q23716) RecName: Full=Elongation factor 2;          Short=EF-2;...   137   1e-31

 psg: 0.71  gvh: 0.38  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHB564 is mit

>VHB564F.Seq
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AATTNNNNNNNNNN