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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE875
Library VH
(Link to library)
Clone ID VHE875
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHE875 (VHE875Q) /CSM/VH/VHE8-D/VHE875Q.Seq.d/
TACAAAATGGTTAATTTCACAATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAAT
ATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCT
TTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCA
TGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAAATCTTCATCTGTATCACTTCAC
TTCAAAATGCCXXXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
YKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVSGDMRYMS
CRADEQERGITIKSSSVSLHFKM---
Translated Amino Acid sequence (All Frames) 
Frame A:
YKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVSGDMRYMS
CRADEQERGITIKSSSVSLHFKM---


Frame B:
tkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpikflvi*dich
vvlmnksvvsplnlhlyhftskc---


Frame C:
qng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*sfw*yeiyvm
sc**trawyhh*ificitslqn---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI722 (VHI722Q) /CSM/VH/VHI7-A/VHI722Q.Seq.d/                        498   e-140
VHE875 (VHE875Q) /CSM/VH/VHE8-D/VHE875Q.Seq.d/                        498   e-140
VHJ703 (VHJ703Q) /CSM/VH/VHJ7-A/VHJ703Q.Seq.d/                        492   e-138
VHC535 (VHC535Q) /CSM/VH/VHC5-B/VHC535Q.Seq.d/                        492   e-138
VHB210 (VHB210Q) /CSM/VH/VHB2-A/VHB210Q.Seq.d/                        492   e-138
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             490   e-138
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        490   e-138
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        490   e-138
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             490   e-138
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        490   e-138
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   490   e-134  1
DY907212|DY907212.1 CHAX1940.b1_G05.ab1 CHA(XYZ) common wild sunflower Helianthus annuus cDNA clone CHAX1940, mRNA sequence.    72   1e-16  3
DY938228|DY938228.1 CHPX5032.b1_O09.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPX5032, mRNA sequence.    72   1e-16  3
DY941362|DY941362.1 CHPX9185.b1_B18.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPX9185, mRNA sequence.    72   1e-16  3
EL476245|EL476245.1 CHUL4181.b1_J14.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL4181, mRNA sequence.    72   1e-16  3
EL513430|EL513430.1 CHUX465.b1_A21.ab1 CHU(XYZ) puzzle sunflower Helianthus paradoxus cDNA clone CHUX465, mRNA sequence.    72   1e-16  3
DY905253|DY905253.1 CHAX11663.b1_N11.ab1 CHA(XYZ) common wild sunflower Helianthus annuus cDNA clone CHAX11663, mRNA sequence.    64   3e-14  3
EK578481|EK578481.1 1095521378025 Global-Ocean-Sampling_GS-32-01-01-1P3-1P6KB marine metagenome genomic clone 1061005950241 5', genomic survey sequence.    52   3e-14  3
DY910258|DY910258.1 CHAX5220.b1_G10.ab1 CHA(XYZ) common wild sunflower Helianthus annuus cDNA clone CHAX5220, mRNA sequence.    64   3e-14  3
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-13  2
dna update 2007. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   117   1e-25
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   113   2e-24
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   112   3e-24
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   112   3e-24
AF534908_1(AF534908|pid:none) Tetrahymena thermophila elongation...   112   3e-24
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   112   3e-24
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   112   4e-24
(O23755) RecName: Full=Elongation factor 2;          Short=EF-2;...   112   4e-24
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   112   5e-24
AY144924_1(AY144924|pid:none) Saccharomyces castellii clone Cont...   111   7e-24
protein update 2009. 7. 2
PSORT 

 psg: 0.48  gvh: 0.38  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHE875 is cyt
5' end seq. ID VHE875F
5' end seq. 
>VHE875F.Seq
TACAAAATGGTTAATTTCACAATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAAT
ATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCT
TTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCA
TGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAAATCTTCATCTGTATCACTTCAC
TTCAAAATGCCNNNNNNNNNN
Length of 5' end seq. 261
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -