>VHF163 (VHF163Q) /CSM/VH/VHF1-C/VHF163Q.Seq.d/
AATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCAAAG
AGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGTATT
GGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTAAAA
TGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGTTGG
GAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAATCA
GCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGTATT
GCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTATGTT
GATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGTCGT
CCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATATCAA
TGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAAAGA
TXXXXXXXXXXACTTGATAAAGATAGTGTAGTTTATAAAACTTTGGTAGATTATGTTGCC
NATAGTCAAGATAAACNATTTAGAACCAATATCTGTGTTCAAGATANTCTTTCACTTGAG
AGAGAGAATGAAAGTGAACGTTTCACTCNTTGGGCAAAAGATAAGAATAGATTACTCTTA
TGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGAATTGCT
CCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCTGATTGT
ATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTTATGTTA
TTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATATGGAAGAG
GCNCCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGNTCNTCACAAAGATGGT
AATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCAAAGACT
GGTCTCTCAACTTCATGTACTCNCNATGAATTCATTGTCTATAAAATTGAACNAGTCAGA
ATCNAATACATACTTAAGAGTTTACATANGAAAATAAAATAAAATTTTTTAAAAT

NSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHLK
CKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDSI
ADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEYQ
CKGWISGFTKCDWKGDSIER---

---LDKDSVVYKTLVDYVAXSQDKXFRTNICVQDXLSLERENESERFTXWAKDKNRLLLW
HGSRLTNFVSIVSQGLRIAPPSAPKTGYRFGKGIYFADCISKSFSYCFTSSDCPTALMLL
CEVSLGDMNELKHDTYMEEAPHPFHSTKALGMAXXHKDGNHPLSDSDGLVVPLGKISKTG
LSTSCTXXEFIVYKIEXVRIXYILKSLHXKIK*nflk

Frame A:
NSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHLK
CKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDSI
ADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEYQ
CKGWISGFTKCDWKGDSIER---

---t**r*csl*nfgrlccx*sr*ti*nqylcsrxsft*ere*k*tfhslgkr*e*itlm
aw**vn*fridclagikncsticsknwlqiw*rylfc*ly**iiqllfhik*lsnctyvi
m*sftw*he*iet*ylygrgxtsip*yksfrygxssqrw*sssf*f*wfsstir*nfkdw
slnfmysx*ihcl*n*tsqnxiht*eftxenkikffk

Frame B:
iakekwqqkihllmrlnmqrvidqhvqpvkevlikkqfvlvikqnqntlmewmyhgii*n
vnvhkyhhlqi*ftgntfvgktnyqlkqlifhlksmiqnqhqrykerni*rhygklkivl
pit*rdqqlktsfniikvmliryhqfiyfilsligc*kvvqvdvqlvrisiyfsmvsnin
vkvgfqvsqnvigkviqlkd---

---LDKDSVVYKTLVDYVAXSQDKXFRTNICVQDXLSLERENESERFTXWAKDKNRLLLW
HGSRLTNFVSIVSQGLRIAPPSAPKTGYRFGKGIYFADCISKSFSYCFTSSDCPTALMLL
CEVSLGDMNELKHDTYMEEAPHPFHSTKALGMAXXHKDGNHPLSDSDGLVVPLGKISKTG
LSTSCTXXEFIVYKIEXVRIXYILKSLHXKIK*nflk

Frame C:
*qkkngnkkyisl*d*icke**inmfnlskry**rsssywl*nkiktl*wngcimasfkm
*mststiiyrfnslgipslgrpiin*nnlffi*kv*skisirgtkreifkgimgs*r*yc
r*legtsn*khhsi**rlc**gitssftsysl*ldvkrssr*msnl*efrytfqwyrism
*rldfrfhkm*ler*fn*k---

---likiv*fiklw*imlpivkinxlepisvfkixfhlrermkvnvslxgqkiridysyg
mvvg*lisyrlsrrd*ellhhllqklvtdlvkvfillivlvnhsaivshqvivqlhlcyy
vkfhlvt*mn*nmipiwkrxhihsivqkl*vwlxxtkmviilflilmv**yh*vkfqrlv
sqlhvlxmnslsiklnxsesntylrvyixk*nkif*n

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF163 (VHF163Q) /CSM/VH/VHF1-C/VHF163Q.Seq.d/                       2357   0.0
VHM584 (VHM584Q) /CSM/VH/VHM5-D/VHM584Q.Seq.d/                       1191   0.0
VHH215 (VHH215Q) /CSM/VH/VHH2-A/VHH215Q.Seq.d/                       1191   0.0
VFM520 (VFM520Q) /CSM/VF/VFM5-A/VFM520Q.Seq.d/                       1191   0.0
SHJ436 (SHJ436Q) /CSM/SH/SHJ4-B/SHJ436Q.Seq.d/                       1191   0.0
SHI546 (SHI546Q) /CSM/SH/SHI5-B/SHI546Q.Seq.d/                       1191   0.0
SHI485 (SHI485Q) /CSM/SH/SHI4-D/SHI485Q.Seq.d/                       1191   0.0
SHG666 (SHG666Q) /CSM/SH/SHG6-C/SHG666Q.Seq.d/                       1191   0.0
SHF645 (SHF645Q) /CSM/SH/SHF6-B/SHF645Q.Seq.d/                       1191   0.0
SHF241 (SHF241Q) /CSM/SH/SHF2-B/SHF241Q.Seq.d/                       1191   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1191   0.0    1
AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).    62   4e-14  3
DY229813|DY229813.1 ID0AAK8YC07CM1 ApHL3SD Acyrthosiphon pisum cDNA clone ID0AAK8YC07 5', mRNA sequence.    60   2e-04  1
AM642769|AM642769.1 Entamoeba dispar GSS, clone dispar98f12.p1k.    54   3e-04  2
EB501254|EB501254.1 1099435318866 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018595867, mRNA sequence.    56   0.003  1
EB500928|EB500928.1 1099435305233 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018598938, mRNA sequence.    56   0.003  1
EB493727|EB493727.1 1099341581900 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018595867, mRNA sequence.    56   0.003  1
EB489445|EB489445.1 1099341224857 12-DrosW-norm-1P5Kb Drosophila willistoni cDNA clone 1061018544138, mRNA sequence.    56   0.003  1
EB455916|EB455916.1 1099341378745 12-DrosW-std-1P6Kb Drosophila willistoni cDNA clone 1061018613759, mRNA sequence.    56   0.003  1
BV472981|BV472981.1 G591P618821RD3.T0 Clint Pan troglodytes verus STS genomic, sequence tagged site.    48   0.004  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK303340_1(AK303340|pid:none) Homo sapiens cDNA FLJ53442 complet...   150   7e-35
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   150   7e-35
J03473_1(J03473|pid:none) Human poly(ADP-ribose) synthetase mRNA...   150   7e-35
AB384394_1(AB384394|pid:none) Synthetic construct DNA, clone: pF...   150   7e-35
M17081_1(M17081|pid:none) Human placental poly(ADP-ribose) polym...   150   7e-35
M18112_1(M18112|pid:none) Human poly(ADP-ribose) polymerase mRNA...   150   7e-35
DQ891636_1(DQ891636|pid:none) Synthetic construct clone IMAGE:10...   150   7e-35
X65497_1(X65497|pid:none) R.norvegicus mRNA for poly(ADP-ribose)...   149   2e-34
(P27008) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   149   2e-34
BT071886_1(BT071886|pid:none) Salmo salar clone ssal-rgf-502-010...   149   2e-34

 psg: 0.58  gvh: 0.23  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for VHF163 is nuc

>VHF163F.Seq
AATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCAAAG
AGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGTATT
GGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTAAAA
TGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGTTGG
GAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAATCA
GCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGTATT
GCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTATGTT
GATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGTCGT
CCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATATCAA
TGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAAAGA
TNNNNNNNNNN

>VHF163Z.Seq
NNNNNNNNNNACTTGATAAAGATAGTGTAGTTTATAAAACTTTGGTAGATTATGTTGCCN
ATAGTCAAGATAAACNATTTAGAACCAATATCTGTGTTCAAGATANTCTTTCACTTGAGA
GAGAGAATGAAAGTGAACGTTTCACTCNTTGGGCAAAAGATAAGAATAGATTACTCTTAT
GGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGAATTGCTC
CACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCTGATTGTA
TTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTTATGTTAT
TATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATATGGAAGAGG
CNCCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGNTCNTCACAAAGATGGTA
ATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCAAAGACTG
GTCTCTCAACTTCATGTACTCNCNATGAATTCATTGTCTATAAAATTGAACNAGTCAGAA
TCNAATACATACTTAAGAGTTTACATANGAAAATAAAATAAAATTTTTTAAAAT

>VHF163P.Seq
AATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCAAAG
AGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGTATT
GGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTAAAA
TGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGTTGG
GAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAATCA
GCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGTATT
GCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTATGTT
GATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGTCGT
CCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATATCAA
TGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAAAGA
T----------ACTTGATAAAGATAGTGTAGTTTATAAAACTTTGGTAGATTATGTTGCC
NATAGTCAAGATAAACNATTTAGAACCAATATCTGTGTTCAAGATANTCTTTCACTTGAG
AGAGAGAATGAAAGTGAACGTTTCACTCNTTGGGCAAAAGATAAGAATAGATTACTCTTA
TGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGAATTGCT
CCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCTGATTGT
ATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTTATGTTA
TTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATATGGAAGAG
GCNCCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGNTCNTCACAAAGATGGT
AATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCAAAGACT
GGTCTCTCAACTTCATGTACTCNCNATGAATTCATTGTCTATAAAATTGAACNAGTCAGA
ATCNAATACATACTTAAGAGTTTACATANGAAAATAAAATAAAATTTTTTAAAAT