>AFO458 (AFO458Q) /CSM/AF/AFO4-C/AFO458Q.Seq.d/
XXXXXXXXXXANTANAGGTATAATTCCCTACAATTATNAAATGGNTAAAATGTTTCCAGA
TCATCNTCAATATAGTTTTTNCTCTACAAATACTGCGAGCAATATGTTATTGCCAAAGTA
TCGTCAGTGGCTACAAGAATTTATTTATAATTTTATTAGTTCAACTGTCACAATTTCCTG
TATATATAATAATCAAATCTTCAACAACAACAACAACAACAACAACAACAACAACAACAA
CAATAATAATAATAATAATAATGATAATAATAATAATAATAATAATACCACCACCTTTAA
TAATAGTCAATATCAATTAGTTGAAATCGTCTTTAGTAGGGATGAATTATCAGCCGTAGA
GTATGAACATTTATTAGATTTTATGGTTGGTGAATTTTATAGAGCCTTT

---XGIIPYNYXMXKMFPDHXQYSFXSTNTASNMLLPKYRQWLQEFIYNFISSTVTISCI
YNNQIFNNNNNNNNNNNNNNNNNNNNDNNNNNNNTTTFNNSQYQLVEIVFSRDELSAVEY
EHLLDFMVGEFYRAF

Frame A:
---xxv*fptixkwxkcfqiixnivfxlqilraicycqsivsgyknlfiillvqlsqfpv
yiiiksstttttttttttttiiiiiimiiiiiiiipppliivnin*lksslvgmnyqp*s
mniy*ilwlvnfiep


Frame B:
---xrynslqlxng*nvsrsssi*fxlykyceqyviakvssvatriyl*fy*fnchnfly
i**snlqqqqqqqqqqqqqq**************yhhl***sisis*nrl**g*iisrrv
*tfirfygw*il*sl


Frame C:
---XGIIPYNYXMXKMFPDHXQYSFXSTNTASNMLLPKYRQWLQEFIYNFISSTVTISCI
YNNQIFNNNNNNNNNNNNNNNNNNNNDNNNNNNNTTTFNNSQYQLVEIVFSRDELSAVEY
EHLLDFMVGEFYRAF

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ432 (VSJ432Q) /CSM/VS/VSJ4-B/VSJ432Q.Seq.d/                        345   3e-94
AFO458 (AFO458Q) /CSM/AF/AFO4-C/AFO458Q.Seq.d/                        345   3e-94
VSJ704 (VSJ704Q) /CSM/VS/VSJ7-A/VSJ704Q.Seq.d/                         38   0.10
VHI820 (VHI820Q) /CSM/VH/VHI8-A/VHI820Q.Seq.d/                         38   0.10
VHI242 (VHI242Q) /CSM/VH/VHI2-B/VHI242Q.Seq.d/                         38   0.10
VHA558 (VHA558Q) /CSM/VH/VHA5-C/VHA558Q.Seq.d/                         38   0.10
SLB233 (SLB233Q) /CSM/SL/SLB2-B/SLB233Q.Seq.d/                         38   0.10
SHK632 (SHK632Q) /CSM/SH/SHK6-B/SHK632Q.Seq.d/                         38   0.10
SHK591 (SHK591Q) /CSM/SH/SHK5-D/SHK591Q.Seq.d/                         38   0.10
SHJ851 (SHJ851Q) /CSM/SH/SHJ8-C/SHJ851Q.Seq.d/                         38   0.10

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC135237|AC135237.2 Mus musculus chromosome UNK clone RP23-306F11, WORKING DRAFT SEQUENCE, 62 unordered pieces.    46  0.33  1
AC010927|AC010927.5 Arabidopsis thaliana chromosome III BAC T22K18 genomic sequence, complete sequence.    46  0.33  1
AL845296|AL845296.4 Mouse DNA sequence from clone RP23-121B24 on chromosome 2.    46  0.35  4
AC140246|AC140246.1 Mus musculus chromosome UNK clone RP23-295K16, WORKING DRAFT SEQUENCE, 5 unordered pieces.    46  1.2  5
AC117821|AC117821.3 Mus musculus clone RP24-576O2, WORKING DRAFT SEQUENCE, 2 ordered pieces.    44  1.3  1
AZ547945|AZ547945.1 ENTGR16TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44  1.3  1
AZ523268|AZ523268.1 217PbD08 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    44  1.3  1
T09643|T09643.1 0215m7 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 0215m, DNA sequence.    38  1.5  2
AA550553|AA550553.1 1716m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 1716m, DNA sequence.    38  1.8  2
AX346723|AX346723.1 Sequence 1794 from Patent WO0200928.          36  2.9  2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 438,970,104

 psg: 0.65  gvh: 0.49  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for AFO458 is cyt

>AFO458Z.Seq
----------ANTANAGGTATAATTCCCTACAATTATNAAATGGNTAAAATGTTTCCAGA
TCATCNTCAATATAGTTTTTNCTCTACAAATACTGCGAGCAATATGTTATTGCCAAAGTA
TCGTCAGTGGCTACAAGAATTTATTTATAATTTTATTAGTTCAACTGTCACAATTTCCTG
TATATATAATAATCAAATCTTCAACAACAACAACAACAACAACAACAACAACAACAACAA
CAATAATAATAATAATAATAATGATAATAATAATAATAATAATAATACCACCACCTTTAA
TAATAGTCAATATCAATTAGTTGAAATCGTCTTTAGTAGGGATGAATTATCAGCCGTAGA
GTATGAACATTTATTAGATTTTATGGTTGGTGAATTTTATAGAGCCTTT