NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD113
Library VS
(Link to library)
Clone ID VSD113
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16602-1
Original site URL
Representative seq. ID VSD113E
(Link to Original site)
Representative DNA sequence 
>VSD113 (VSD113Q) /CSM/VS/VSD1-A/VSD113Q.Seq.d/
AATAATAATAAATAAATAAATAAAATGAATATACTTAAAGCTTTAGTAGTTTTATGTTTT
GTTTACGTTTCAATGGGTATCAAAGTTGATACTCAAACTGGTTTGAGATATACCAGAGAA
TCATGTTATAAAAAGAGTGATAATCAACAAAGAGAACTCATTTTATCAACTCAACCAAAA
GATATGAATCTTGAAGTTCCACAATCATGGGATTGGAGAAATGTAAGTGGTGTCAATTAT
CTCACAATGAATCGTAATCAACATATTCCACAATATTGTGGTGGTTGTTGGGCTTTCGCT
TCAACAAGTTCTATCTCTGATAGAATTAAAATTCAACGTAAAGCTGCTTTCCCAGATGTC
AACGTTGCTCCACAACATCTTATTGATTGTAATGGTGGTGGTACATGTGATGGTGGTGAC
CCAGGTGATGCTTTCGCTTTTATCAATGAAAATGGTATTGTTGATGAAACTTGCAAACCA
TACCAAGCTAAAAATTTACCAGACGAATGTTCACCAGCTTGCAAAACCTGTAATCCAGAT
GGTACTTGTCAAGCTATTCCAGTTCATACCAATATCACTGTAACTGAATATGGTTCAGTT
AGAGGTGCTAAAGATATGATGGCTGAAATTTATGCTCGTGGTCCAATCGCTTGTTCAATT
GATGCTACCTCTAAATTAGAAGCCTATACCTCAGGTATCTTCAAGGAATTCAAACTCGAC
CCACTTCCAAATCACATCATCAGTGTTATTGGTTGGGGTGTACAAGACTCAACTCCATAT
TGGATCGTTCGTAATTCTTGGGGTAGTTATTATGGTGAAGGTGGTTTCTTCAACATTGTC
CAAGGTTCTCTTTTCGAAAATTTAGGTATTGAGTTAGACTGTAACTGGGCTGTTCCATCT
GTATCTTTGCTTAAACTAAATAAATAAAATTATCATCATCGATT
sequence update 2001. 3.25
Translated Amino Acid sequence 
nnnk*INKMNILKALVVLCFVYVSMGIKVDTQTGLRYTRESCYKKSDNQQRELILSTQPK
DMNLEVPQSWDWRNVSGVNYLTMNRNQHIPQYCGGCWAFASTSSISDRIKIQRKAAFPDV
NVAPQHLIDCNGGGTCDGGDPGDAFAFINENGIVDETCKPYQAKNLPDECSPACKTCNPD
GTCQAIPVHTNITVTEYGSVRGAKDMMAEIYARGPIACSIDATSKLEAYTSGIFKEFKLD
PLPNHIISVIGWGVQDSTPYWIVRNSWGSYYGEGGFFNIVQGSLFENLGIELDCNWAVPS
VSLLKLNK*nyhhr
Translated Amino Acid sequence (All Frames) 
Frame A:
nnnk*INKMNILKALVVLCFVYVSMGIKVDTQTGLRYTRESCYKKSDNQQRELILSTQPK
DMNLEVPQSWDWRNVSGVNYLTMNRNQHIPQYCGGCWAFASTSSISDRIKIQRKAAFPDV
NVAPQHLIDCNGGGTCDGGDPGDAFAFINENGIVDETCKPYQAKNLPDECSPACKTCNPD
GTCQAIPVHTNITVTEYGSVRGAKDMMAEIYARGPIACSIDATSKLEAYTSGIFKEFKLD
PLPNHIISVIGWGVQDSTPYWIVRNSWGSYYGEGGFFNIVQGSLFENLGIELDCNWAVPS
VSLLKLNK*nyhhr


Frame B:
iiink*ik*iylkl**fyvlftfqwvsklilklv*dipenhvikrviinkensfyqlnqk
i*ilkfhnhgigem*vvsiisq*ivinifhnivvvvglslqqvlslielkfnvkllsqms
tllhnillivmvvvhvmvvtqvmlsllsmkmvllmklanhtklkiyqtnvhqlakpviqm
vlvklfqfipisl*lnmvqlevlki*wlkfmlvvqslvqlmlpln*kpipqvssrnsnst
hfqitssvllvgvyktqlhigsfvilgvvimvkvvsstlskvlfski*vls*tvtglfhl
ylcln*inkiiiid


Frame C:
***ink*neyt*sfssfmfclrfngyqs*ysnwfeiyqriml*ke**stkrthfinstkr
yes*sstimglekckwcqlshnes*stystilwwllgfrfnkfyl**n*nst*scfprcq
rcsttsy*l*wwwym*ww*pr*cfrfyq*kwyc**nlqtips*kftrrmftslqnl*srw
ylssysssyqyhcn*iwfs*rc*rydg*nlcswsnrlfn*cyl*irslylrylqgiqtrp
tskshhqcywlgctrlnsildrs*flg*llw*rwflqhcprfsfrkfry*vrl*lgcsic
ifa*tk*iklsssi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD113 (VSD113Q) /CSM/VS/VSD1-A/VSD113Q.Seq.d/                       1806   0.0
VFD780 (VFD780Q) /CSM/VF/VFD7-D/VFD780Q.Seq.d/                       1800   0.0
VSF638 (VSF638Q) /CSM/VS/VSF6-B/VSF638Q.Seq.d/                       1740   0.0
VFA818 (VFA818Q) /CSM/VF/VFA8-A/VFA818Q.Seq.d/                       1699   0.0
SSC608 (SSC608Q) /CSM/SS/SSC6-A/SSC608Q.Seq.d/                       1608   0.0
VSE347 (VSE347Q) /CSM/VS/VSE3-B/VSE347Q.Seq.d/                       1283   0.0
SSA582 (SSA582Q) /CSM/SS/SSA5-D/SSA582Q.Seq.d/                       1263   0.0
SSD547 (SSD547Q) /CSM/SS/SSD5-B/SSD547Q.Seq.d/                       1037   0.0
FC-BL05 (FC-BL05Q) /CSM/FC/FC-BL/FC-BL05Q.Seq.d/                     1019   0.0
VHF749 (VHF749Q) /CSM/VH/VHF7-C/VHF749Q.Seq.d/                        769   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG224657|BG224657.1 kp49c02.y1 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to WP:F32B5.8 CE09855 CYSTEINE PROTEINASE ;, mRNA sequence.    50  7e-07  3
BI743363|BI743363.1 kx43b03.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:O01850 O01850 SIMILAR TO CYSTEINE PROTEINASE. [1] ;, mRNA sequence.    62  1e-05  1
BI743660|BI743660.1 kx50c01.y1 Parastrongyloides trichosuri PA pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:Q9XZI2 Q9XZI2 CATHEPSIN Z1 PREPROPROTEIN. ;, mRNA sequence.    62  1e-05  1
BI501371|BI501371.1 kx31d07.y1 Parastrongyloides trichosuri PA pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:O01850 O01850 SIMILAR TO CYSTEINE PROTEINASE. [1] ;, mRNA sequence.    62  1e-05  1
U71150|U71150.1 Onchocerca volvulus cysteine protease precursor mRNA, complete cds.    46  3e-05  3
BP522506|BP522506.1 Hydra magnipapillata cDNA, clone:hmp_20381.    40  0.001  2
BP509588|BP509588.1 Hydra magnipapillata cDNA, clone:hmp_04626.    38  0.069  2
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    36  0.65  4
AL929507|AL929507.10 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-7C18.    46  0.79  1
CF514057|CF514057.1 CAbud0007_IVR_A11 Vitis vinifera cv. cabernet sauvignon (Clone 8) Bud - CABUD Vitis vinifera cDNA clone CAbud0007_IVR_A11 3', mRNA sequence.    46  0.79  1
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY950579_1(AY950579|pid:none) Paralichthys olivaceus cathepsin Z...   234   2e-60
AY226449_1(AY226449|pid:none) Fundulus heteroclitus cathepsin Z ...   231   2e-59
BT075594_1(BT075594|pid:none) Osmerus mordax clone omor-eva-517-...   230   5e-59
AY890922_1(AY890922|pid:none) Synthetic construct Homo sapiens c...   228   2e-58
(Q9UBR2) RecName: Full=Cathepsin Z;          EC=3.4.18.1; AltNam...   228   2e-58
AF009923_1(AF009923|pid:none) Homo sapiens preprocathepsin P mRN...   228   2e-58
AY891244_1(AY891244|pid:none) Synthetic construct Homo sapiens c...   228   2e-58
AY949988_1(AY949988|pid:none) Cyprinus carpio cathepsin Z (CTPZ)...   228   2e-58
BT019915_1(BT019915|pid:none) Homo sapiens cathepsin Z mRNA, com...   228   3e-58
AF032906_1(AF032906|pid:none) Homo sapiens cathepsin Z precursor...   227   4e-58
protein update 2009. 7.29
PSORT 

 psg: 0.97  gvh: 0.69  alm: 0.44  top: 0.40  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi

>> prediction for VSD113 is exc
5' end seq. ID VSD113F
5' end seq. 
>VSD113F.Seq
AATAATAATAAATAAATAAATAAAATGAATATACTTAAAGCTTTAGTAGTTTTATGTTTT
GTTTACGTTTCAATGGGTATCAAAGTTGATACTCAAACTGGTTTGAGATATACCAGAGAA
TCATGTTATAAAAAGAGTGATAATCAACAAAGAGAACTCATTTTATCAACTCAACCAAAA
GATATGAATCTTGAAGTTCCACAATCATGGGATTGGAGAAATGTAAGTGGTGTCAATTAT
CTCACAATGAATCGTAATCAACATATTCCACAATATTGTGGTGGTTGTTGGGCTTTCGCT
TCAACAAGTTCTATCTCTGATAGAATTAAAATTCAACGTAAAGCTGCTTTCCCAGATGTC
AACGTTGCTCCACAACATCTTATTGATTGTAATGGTGGTGGTACATGTGATGGTGGTGAC
CCAGGTGATGCTTTCGCTTTTATCAATGAAAATGGTATTGTTGATGAAACTTGCAAACCA
TACCAAGCTAAAAATTTACCAGACGAATGTTCACCAGCTTGCAAAACCTGTAATCCAGAT
GGTACTTGTCAAGCT----------
Length of 5' end seq. 555
3' end seq. ID VSD113Z
3' end seq. 
>VSD113Z.Seq
----------TGTAAGTGGTGTCAATTATCTCACAATGAATCGTAATCAACATATTCCAC
AATATTGTGGTGGTTGTTGGGCTTTCGCTTCAACAAGTTCTATCTCTGATAGAATTAAAA
TTCAACGTAAAGCTGCTTTCCCAGATGTCAACGTTGCTCCACAACATCTTATTGATTGTA
ATGGTGGTGGTACATGTGATGGTGGTGACCCAGGTGATGCTTTCGCTTTTATCAATGAAA
ATGGTATTGTTGATGAAACTTGCAAACCATACCAAGCTAAAAATTTACCAGACGAATGTT
CACCAGCTTGCAAAACCTGTAATCCAGATGGTACTTGTCAAGCTATTCCAGTTCATACCA
ATATCACTGTAACTGAATATGGTTCAGTTAGAGGTGCTAAAGATATGATGGCTGAAATTT
ATGCTCGTGGTCCAATCGCTTGTTCAATTGATGCTACCTCTAAATTAGAAGCCTATACCT
CAGGTATCTTCAAGGAATTCAAACTCGACCCACTTCCAAATCACATCATCAGTGTTATTG
GTTGGGGTGTACAAGACTCAACTCCATATTGGATCGTTCGTAATTCTTGGGGTAGTTATT
ATGGTGAAGGTGGTTTCTTCAACATTGTCCAAGGTTCTCTTTTCGAAAATTTAGGTATTG
AGTTAGACTGTAACTGGGCTGTTCCATCTGTATCTTTGCTTAAACTAAATAAATAAAATT
ATCATCATCGATT
Length of 3' end seq. 723
Connected seq. ID VSD113P
Connected seq. 
>VSD113P.Seq
AATAATAATAAATAAATAAATAAAATGAATATACTTAAAGCTTTAGTAGTTTTATGTTTT
GTTTACGTTTCAATGGGTATCAAAGTTGATACTCAAACTGGTTTGAGATATACCAGAGAA
TCATGTTATAAAAAGAGTGATAATCAACAAAGAGAACTCATTTTATCAACTCAACCAAAA
GATATGAATCTTGAAGTTCCACAATCATGGGATTGGAGAAATGTAAGTGGTGTCAATTAT
CTCACAATGAATCGTAATCAACATATTCCACAATATTGTGGTGGTTGTTGGGCTTTCGCT
TCAACAAGTTCTATCTCTGATAGAATTAAAATTCAACGTAAAGCTGCTTTCCCAGATGTC
AACGTTGCTCCACAACATCTTATTGATTGTAATGGTGGTGGTACATGTGATGGTGGTGAC
CCAGGTGATGCTTTCGCTTTTATCAATGAAAATGGTATTGTTGATGAAACTTGCAAACCA
TACCAAGCTAAAAATTTACCAGACGAATGTTCACCAGCTTGCAAAACCTGTAATCCAGAT
GGTACTTGTCAAGCT----------TGTAAGTGGTGTCAATTATCTCACAATGAATCGTA
ATCAACATATTCCACAATATTGTGGTGGTTGTTGGGCTTTCGCTTCAACAAGTTCTATCT
CTGATAGAATTAAAATTCAACGTAAAGCTGCTTTCCCAGATGTCAACGTTGCTCCACAAC
ATCTTATTGATTGTAATGGTGGTGGTACATGTGATGGTGGTGACCCAGGTGATGCTTTCG
CTTTTATCAATGAAAATGGTATTGTTGATGAAACTTGCAAACCATACCAAGCTAAAAATT
TACCAGACGAATGTTCACCAGCTTGCAAAACCTGTAATCCAGATGGTACTTGTCAAGCTA
TTCCAGTTCATACCAATATCACTGTAACTGAATATGGTTCAGTTAGAGGTGCTAAAGATA
TGATGGCTGAAATTTATGCTCGTGGTCCAATCGCTTGTTCAATTGATGCTACCTCTAAAT
TAGAAGCCTATACCTCAGGTATCTTCAAGGAATTCAAACTCGACCCACTTCCAAATCACA
TCATCAGTGTTATTGGTTGGGGTGTACAAGACTCAACTCCATATTGGATCGTTCGTAATT
CTTGGGGTAGTTATTATGGTGAAGGTGGTTTCTTCAACATTGTCCAAGGTTCTCTTTTCG
AAAATTTAGGTATTGAGTTAGACTGTAACTGGGCTGTTCCATCTGTATCTTTGCTTAAAC
TAAATAAATAAAATTATCATCATCGATT
Length of connected seq. 1278
Full length Seq ID VSD113E
Full length Seq. 
>VSD113E.Seq
AATAATAATAAATAAATAAATAAAATGAATATACTTAAAGCTTTAGTAGTTTTATGTTTT
GTTTACGTTTCAATGGGTATCAAAGTTGATACTCAAACTGGTTTGAGATATACCAGAGAA
TCATGTTATAAAAAGAGTGATAATCAACAAAGAGAACTCATTTTATCAACTCAACCAAAA
GATATGAATCTTGAAGTTCCACAATCATGGGATTGGAGAAATGTAAGTGGTGTCAATTAT
CTCACAATGAATCGTAATCAACATATTCCACAATATTGTGGTGGTTGTTGGGCTTTCGCT
TCAACAAGTTCTATCTCTGATAGAATTAAAATTCAACGTAAAGCTGCTTTCCCAGATGTC
AACGTTGCTCCACAACATCTTATTGATTGTAATGGTGGTGGTACATGTGATGGTGGTGAC
CCAGGTGATGCTTTCGCTTTTATCAATGAAAATGGTATTGTTGATGAAACTTGCAAACCA
TACCAAGCTAAAAATTTACCAGACGAATGTTCACCAGCTTGCAAAACCTGTAATCCAGAT
GGTACTTGTCAAGCTATTCCAGTTCATACCAATATCACTGTAACTGAATATGGTTCAGTT
AGAGGTGCTAAAGATATGATGGCTGAAATTTATGCTCGTGGTCCAATCGCTTGTTCAATT
GATGCTACCTCTAAATTAGAAGCCTATACCTCAGGTATCTTCAAGGAATTCAAACTCGAC
CCACTTCCAAATCACATCATCAGTGTTATTGGTTGGGGTGTACAAGACTCAACTCCATAT
TGGATCGTTCGTAATTCTTGGGGTAGTTATTATGGTGAAGGTGGTTTCTTCAACATTGTC
CAAGGTTCTCTTTTCGAAAATTTAGGTATTGAGTTAGACTGTAACTGGGCTGTTCCATCT
GTATCTTTGCTTAAACTAAATAAATAAAATTATCATCATCGATT
Length of full length seq. 944