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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD124
Library VS
(Link to library)
Clone ID VSD124
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U03055-1
Original site URL
Representative seq. ID VSD124P
(Link to Original site)
Representative DNA sequence 
>VSD124 (VSD124Q) /CSM/VS/VSD1-A/VSD124Q.Seq.d/
CCCGCGTCCGAATTATTCTAAATAAAAATGGCATCACAAGAAAGTTTAACTGAAGAACAA
ATTGCTGAATTCAAAGAAGCATTCTCTTTATTCGATAAAGATGGTGATGGTTCAATTACC
ACCAAAGAATTAGGTACTGTTATGAGATCATTAGGTCAAAACCCAACTGAAGCTGAATTA
CAAGATATGATCAATGAAGTTGACGCTGATGGTAATGGTAACATTGATTTCCCAGAATTT
TTAACCATGATGGCTCGTAAAATGCAAGACACTGATACTGAAGAAGAAATCCGTGAAGCA
TTCAAAGTTTTCGATAAAGACGGTAACGGTTACATCTCAGCTGCTGAACTTAGACACGTT
ATGACCAGTCTTGGTGAAAAACTTACCAACGAAGAAGTTGATGAAATGATTAGAGAAGCT
GATTTGGATGGCGATGGTCAAGTTAACTATGACGAATTTGTTAAAATGATGATTGTTAGA
AATTAAGAAAATTTAATATTAAAAAAAAACAAAAACCTTGAAAAAAAAAAXXXXXXXXXX
ATTATTCATAAATAAAAATGGCATCNCAAGAAAGTTTAANTGAAGANCAAATTGNTGAAT
TCAAAGAAGCATTCTCTTTATTCGATAAAGATGGTGATGGTTCAATTACCACCAAAGAAT
TAGGNNCTGTTATGAGATCATTAGGTCAAAACNCNACTNAAGCTGAATTACAAGATATGA
TCAATGAAGTTGACGCTGATGGTAATGGTAACATTGATTTCCCAGAATTTTTAACCATGA
TGGCTCGTAAAATGCAAGACACTGATACTGAAGAAGAAACCCGTGAAGCATTCAAAGTTT
TCGATAAAGACGGTAACGGTTACATCTCAGCTGCTGAATCTTAGACACGTNATGACCAGT
CTTGGTGAAAAACTNACCAACGAAGAAGTTGATGAAATGATTAGAGAAGCTGATTTGGAT
GGCGATGGTCCNGTTAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
paself*ikmasqeslteeqiaefkeafslfdkdgdgsittkelgtvmrslgqnpteael
qdminevdadgngnidfpefltmmarkmqdtdteeeireafkvfdkdgngyisaaelrhv
mtslgekltneevdemireadldgdgqvnydefvkmmivrn*ENLILKKNKNLEKK---

---LFINKNGIXRKFX*rxnx*iqrsilfir*rw*wfnyhqrirxcyeiirskxxxs*it
rydq*s*r*w*w*h*fprifnhdgs*narh*y*rrnp*siqsfr*rr*rlhlsc*ildtx
*pvlvknxptkklmk*lekliwmamvxl
Translated Amino Acid sequence (All Frames) 
Frame A:
paself*ikmasqeslteeqiaefkeafslfdkdgdgsittkelgtvmrslgqnpteael
qdminevdadgngnidfpefltmmarkmqdtdteeeireafkvfdkdgngyisaaelrhv
mtslgekltneevdemireadldgdgqvnydefvkmmivrn*ENLILKKNKNLEKK---

---iihk*kwhxkkv*xkxklxnskkhslysikmvmvqlppkn*xll*dh*vktxlklny
ki*smkltlmvmvtlisqnf*p*wlvkcktlilkkkpvkhskfsiktvtvtsqllnlrhv
mtslgekltneevdemireadldgdgpv

Frame B:
prpnysk*kwhhkkv*lknkllnskkhslysikmvmvqlppkn*vll*dh*vktqlklny
ki*smkltlmvmvtlisqnf*p*wlvkcktlilkkksvkhskfsiktvtvtsqllnldtl
*pvlvknlptkklmk*lekliwmamvkltmtnllk**lleikki*y*kktktlkkk---

---LFINKNGIXRKFX*rxnx*iqrsilfir*rw*wfnyhqrirxcyeiirskxxxs*it
rydq*s*r*w*w*h*fprifnhdgs*narh*y*rrnp*siqsfr*rr*rlhlsc*ildtx
*pvlvknxptkklmk*lekliwmamvxl

Frame C:
rvriilnkngitrkfn*rtnc*iqrsilfir*rw*wfnyhqrirycyeiirskpn*s*it
rydq*s*r*w*w*h*fprifnhdgs*narh*y*rrnp*siqsfr*rr*rlhlsc*t*try
dqsw*ktyqrrs**nd*rs*fgwrwss*l*ric*nddc*klrkfnikkkqkp*kkk---

---ys*ikmasqeslxexqixefkeafslfdkdgdgsittkelgxvmrslgqnxtxaelq
dminevdadgngnidfpefltmmarkmqdtdteeetreafkvfdkdgngyisaaes*trx
dqsw*ktxqrrs**nd*rs*fgwrwsx*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD124 (VSD124Q) /CSM/VS/VSD1-A/VSD124Q.Seq.d/                        991   0.0
SSC370 (SSC370Q) /CSM/SS/SSC3-C/SSC370Q.Seq.d/                        957   0.0
SSI423 (SSI423Q) /CSM/SS/SSI4-A/SSI423Q.Seq.d/                        955   0.0
SSF835 (SSF835Q) /CSM/SS/SSF8-B/SSF835Q.Seq.d/                        954   0.0
SSG339 (SSG339Q) /CSM/SS/SSG3-B/SSG339Q.Seq.d/                        952   0.0
SSJ721 (SSJ721Q) /CSM/SS/SSJ7-A/SSJ721Q.Seq.d/                        948   0.0
VSI787 (VSI787Q) /CSM/VS/VSI7-D/VSI787Q.Seq.d/                        942   0.0
SSC609 (SSC609Q) /CSM/SS/SSC6-A/SSC609Q.Seq.d/                        934   0.0
SSA510 (SSA510Q) /CSM/SS/SSA5-A/SSA510Q.Seq.d/                        932   0.0
SSL130 (SSL130Q) /CSM/SS/SSL1-B/SSL130Q.Seq.d/                        928   0.0
own update 2001.11.12
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M64089|M64089.1 Dictyostelium discoideum calmodulin (calA) gene, complete cds.   484  0.0  5
M13009|M13009.1 D.discoideum calmodulin mRNA, partial cds.       852  0.0  2
D10521|D10521.1 T.pyriformis mRNA for calmodulin.                309  4e-80  2
CB073854|CB073854.1 taa21c03.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:O96102 O96102 CALMODULIN. ;, mRNA sequence.   206  1e-78  2
X52242|X52242.1 T.thermophila mRNA for calmodulin.               301  9e-78  2
BM394651|BM394651.1 50072-2-5-C06.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   301  9e-78  2
BG817582|BG817582.1 ESSU0004 S.scabiei cDNA library Sarcoptes scabiei cDNA clone SAS0005 5', mRNA sequence.   283  2e-72  2
CB930182|CB930182.1 ri71h04.y1 Meloidogyne chitwoodi J2 pDNR LIB Meloidogyne chitwoodi cDNA 5' similar to SW:CALM_DROME P07181 CALMODULIN. [8] SW:CALM_DROME TR:O02367 TR:O96081 TR:O97341 TR:O16305 TR:Q9V3T4 TR:Q9UB37 ;, mRNA sequence.   149  2e-66  3
CB073563|CB073563.1 taa17g12.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to SW:CALM_METSE P02596 CALMODULIN. ;, mRNA sequence.   163  1e-65  2
CB929995|CB929995.1 ri69d12.y1 Meloidogyne chitwoodi J2 pDNR LIB Meloidogyne chitwoodi cDNA 5' similar to SW:CALM_DROME P07181 CALMODULIN. [8] SW:CALM_DROME TR:O02367 TR:O96081 TR:O97341 TR:O16305 TR:Q9V3T4 TR:Q9UB37 ;, mRNA sequence.   141  3e-64  3
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M13009_1(M13009|pid:none) D.discoideum calmodulin mRNA, partial ...   276   8e-73
DQ457005_1(DQ457005|pid:none) Synthetic construct plasmid pN1-4n...   271   3e-71
AY891413_1(AY891413|pid:none) Synthetic construct Homo sapiens c...   270   6e-71
AY894058_1(AY894058|pid:none) Synthetic construct Homo sapiens c...   270   6e-71
AX441271_1(AX441271|pid:none) Sequence 2 from Patent EP1209167. ...   270   6e-71
(Q95NR9) RecName: Full=Calmodulin;          Short=CaM;  &AB06318...   270   7e-71
MCSFCU(JK0011)calmodulin - sea cucumber (Stichopus japonicus)         269   1e-70
AK152715_1(AK152715|pid:none) Mus musculus bone marrow macrophag...   269   1e-70
(P21251) RecName: Full=Calmodulin;          Short=CaM;                269   1e-70
BC008437_1(BC008437|pid:none) Homo sapiens calmodulin 2 (phospho...   269   1e-70
protein update 2009. 7.29
PSORT 

 psg: 0.57  gvh: 0.08  alm: 0.69  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VSD124 is nuc
5' end seq. ID VSD124F
5' end seq. 
>VSD124F.Seq
CCCGCGTCCGAATTATTCTAAATAAAAATGGCATCACAAGAAAGTTTAACTGAAGAACAA
ATTGCTGAATTCAAAGAAGCATTCTCTTTATTCGATAAAGATGGTGATGGTTCAATTACC
ACCAAAGAATTAGGTACTGTTATGAGATCATTAGGTCAAAACCCAACTGAAGCTGAATTA
CAAGATATGATCAATGAAGTTGACGCTGATGGTAATGGTAACATTGATTTCCCAGAATTT
TTAACCATGATGGCTCGTAAAATGCAAGACACTGATACTGAAGAAGAAATCCGTGAAGCA
TTCAAAGTTTTCGATAAAGACGGTAACGGTTACATCTCAGCTGCTGAACTTAGACACGTT
ATGACCAGTCTTGGTGAAAAACTTACCAACGAAGAAGTTGATGAAATGATTAGAGAAGCT
GATTTGGATGGCGATGGTCAAGTTAACTATGACGAATTTGTTAAAATGATGATTGTTAGA
AATTAAGAAAATTTAATATTAAAAAAAAACAAAAACCTTGAAAAAAAAAA----------
Length of 5' end seq. 530
3' end seq. ID VSD124Z
3' end seq. 
>VSD124Z.Seq
----------ATTATTCATAAATAAAAATGGCATCNCAAGAAAGTTTAANTGAAGANCAA
ATTGNTGAATTCAAAGAAGCATTCTCTTTATTCGATAAAGATGGTGATGGTTCAATTACC
ACCAAAGAATTAGGNNCTGTTATGAGATCATTAGGTCAAAACNCNACTNAAGCTGAATTA
CAAGATATGATCAATGAAGTTGACGCTGATGGTAATGGTAACATTGATTTCCCAGAATTT
TTAACCATGATGGCTCGTAAAATGCAAGACACTGATACTGAAGAAGAAACCCGTGAAGCA
TTCAAAGTTTTCGATAAAGACGGTAACGGTTACATCTCAGCTGCTGAATCTTAGACACGT
NATGACCAGTCTTGGTGAAAAACTNACCAACGAAGAAGTTGATGAAATGATTAGAGAAGC
TGATTTGGATGGCGATGGTCCNGTTAA
Length of 3' end seq. 437
Connected seq. ID VSD124P
Connected seq. 
>VSD124P.Seq
CCCGCGTCCGAATTATTCTAAATAAAAATGGCATCACAAGAAAGTTTAACTGAAGAACAA
ATTGCTGAATTCAAAGAAGCATTCTCTTTATTCGATAAAGATGGTGATGGTTCAATTACC
ACCAAAGAATTAGGTACTGTTATGAGATCATTAGGTCAAAACCCAACTGAAGCTGAATTA
CAAGATATGATCAATGAAGTTGACGCTGATGGTAATGGTAACATTGATTTCCCAGAATTT
TTAACCATGATGGCTCGTAAAATGCAAGACACTGATACTGAAGAAGAAATCCGTGAAGCA
TTCAAAGTTTTCGATAAAGACGGTAACGGTTACATCTCAGCTGCTGAACTTAGACACGTT
ATGACCAGTCTTGGTGAAAAACTTACCAACGAAGAAGTTGATGAAATGATTAGAGAAGCT
GATTTGGATGGCGATGGTCAAGTTAACTATGACGAATTTGTTAAAATGATGATTGTTAGA
AATTAAGAAAATTTAATATTAAAAAAAAACAAAAACCTTGAAAAAAAAAA----------
ATTATTCATAAATAAAAATGGCATCNCAAGAAAGTTTAANTGAAGANCAAATTGNTGAAT
TCAAAGAAGCATTCTCTTTATTCGATAAAGATGGTGATGGTTCAATTACCACCAAAGAAT
TAGGNNCTGTTATGAGATCATTAGGTCAAAACNCNACTNAAGCTGAATTACAAGATATGA
TCAATGAAGTTGACGCTGATGGTAATGGTAACATTGATTTCCCAGAATTTTTAACCATGA
TGGCTCGTAAAATGCAAGACACTGATACTGAAGAAGAAACCCGTGAAGCATTCAAAGTTT
TCGATAAAGACGGTAACGGTTACATCTCAGCTGCTGAATCTTAGACACGTNATGACCAGT
CTTGGTGAAAAACTNACCAACGAAGAAGTTGATGAAATGATTAGAGAAGCTGATTTGGAT
GGCGATGGTCCNGTTAA
Length of connected seq. 967
Full length Seq ID -
Full length Seq. -
Length of full length seq. -