>VSD151 (VSD151Q) /CSM/VS/VSD1-C/VSD151Q.Seq.d/
TCCGCAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAATGTT
TTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATTGAA
GGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGTGAA
ACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAATTA
ATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATTCGT
ATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTAAAG
AAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAAAAA
TTCTTTTCAAAATAAATTAAATTCTCTATTCTTTTAATTTTAAAAAAAAAAXXXXXXXXX
XCAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAATGTTTTA
CGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATTGAAGGT
GACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGTGAAACC
AATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAATTAATT
GAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATTCGTATT
ACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTAAAGAAG
GATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAAAAATTC
TTTTCAAAATAAATTAAATTCTCT

sarvqksailnenidkvwnvlrnfdfpskifpviessiiegdstpttvgairvlkwktge
tnkqrllelsdlshkiiyelieseqtaevtayistikliritesnqtlltwesefsadvk
kdlinfesksvslnlqdlqkffsk*IKFSILLILKKK---

---RVQKSAILNENIDKVWNVLRNFDFPSKIFPVIESSIIEGDSTPTTVGAIRVLKWKTG
ETNKQRLLELSDLSHKIIYELIESEQTAEVTAYISTIKLIRITESNQTLLTWESEFSADV
KKDLINFESKSVSLNLQDLQKFFSK*ikfs

Frame A:
sarvqksailnenidkvwnvlrnfdfpskifpviessiiegdstpttvgairvlkwktge
tnkqrllelsdlshkiiyelieseqtaevtayistikliritesnqtlltwesefsadvk
kdlinfesksvslnlqdlqkffsk*IKFSILLILKKK---

---qeyknlqf*mkt*tkygmfyvisishqkssq*skvqllkvtqhqpllepsvf*sgkl
vkpinkdy*nslifhiksfmn*lkvnkllkllptlapln*fvllnqikpf*lgkvnfqqm
*rri*lisnqnqfp*iykiyknsfqnklns

Frame B:
pqeyknlqf*mkt*tkygmfyvisishqkssq*skvqllkvtqhqpllepsvf*sgklvk
pinkdy*nslifhiksfmn*lkvnkllkllptlapln*fvllnqikpf*lgkvnfqqm*r
ri*lisnqnqfp*iykiyknsfqnklnslff*f*kk---

---kstkicnfk*khrqsmecft*frfpiknlpsnrkfny*r*lntnhcwshpcfkvenw
*nq*tkiirtl*sft*nhl*in*k*tnc*syclh*hh*insyy*iksnpfdlgk*ifsrc
kegfn*frikisflkftrftkilfkin*il

Frame C:
rkstkicnfk*khrqsmecft*frfpiknlpsnrkfny*r*lntnhcwshpcfkvenw*n
q*tkiirtl*sft*nhl*in*k*tnc*syclh*hh*insyy*iksnpfdlgk*ifsrcke
gfn*frikisflkftrftkilfkin*ilysfnfkkk---

---RVQKSAILNENIDKVWNVLRNFDFPSKIFPVIESSIIEGDSTPTTVGAIRVLKWKTG
ETNKQRLLELSDLSHKIIYELIESEQTAEVTAYISTIKLIRITESNQTLLTWESEFSADV
KKDLINFESKSVSLNLQDLQKFFSK*ikfs

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD151 (VSD151Q) /CSM/VS/VSD1-C/VSD151Q.Seq.d/                        914   0.0
VSH795 (VSH795Q) /CSM/VS/VSH7-D/VSH795Q.Seq.d/                        906   0.0
VSH527 (VSH527Q) /CSM/VS/VSH5-B/VSH527Q.Seq.d/                        906   0.0
VSE874 (VSE874Q) /CSM/VS/VSE8-D/VSE874Q.Seq.d/                        906   0.0
SSD805 (SSD805Q) /CSM/SS/SSD8-A/SSD805Q.Seq.d/                        906   0.0
AHC765 (AHC765Q) /CSM/AH/AHC7-C/AHC765Q.Seq.d/                        906   0.0
VSC672 (VSC672Q) /CSM/VS/VSC6-C/VSC672Q.Seq.d/                        900   0.0
VSB579 (VSB579Q) /CSM/VS/VSB5-D/VSB579Q.Seq.d/                        886   0.0
AHH891 (AHH891Q) /CSM/AH/AHH8-D/AHH891Q.Seq.d/                        858   0.0
VSF407 (VSF407Q) /CSM/VS/VSF4-A/VSF407Q.Seq.d/                        850   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.   434  0.0  5
CC727726|CC727726.1 OGKAC88TM ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0302P07, genomic survey sequence.    38  0.007  2
CC737524|CC737524.1 ZMMBBb0017E19.f ZMMBBb Zea mays subsp. mays genomic clone ZMMBBb0017E19 5', genomic survey sequence.    38  0.009  3
AE014839|AE014839.1 Plasmodium falciparum 3D7 chromosome 11 section 4 of 8 of the complete sequence.    34  0.018  8
AC000385|AC000385.2 Homo sapiens chromosome 11 clone PAC pDJ392a17, complete sequence.    40  0.018  3
AC068587|AC068587.16 Homo sapiens chromosome 8, clone RP11-303G3, complete sequence.    40  0.020  3
AC116989|AC116989.2 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.    38  0.12  5
AL031633|AL031633.1 Caenorhabditis elegans YAC Y39A1A.            32  0.15  8
AC114681|AC114681.6 Homo sapiens chromosome 8 clone RP11-24D9 map 8, 5 unordered pieces.    40  0.18  3
AC027103|AC027103.1 Homo sapiens chromosome 15 clone RP11-238E5 map 15, LOW-PASS SEQUENCE SAMPLING.    38  0.22  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000393_3865(CP000393|pid:none) Trichodesmium erythraeum IMS101...    72   2e-11
BA000040_3379(BA000040|pid:none) Bradyrhizobium japonicum USDA 1...    71   5e-11
CU234118_4532(CU234118|pid:none) Bradyrhizobium sp. ORS278,compl...    70   8e-11
CP000494_4531(CP000494|pid:none) Bradyrhizobium sp. BTAi1, compl...    67   7e-10
CP000085_877(CP000085|pid:none) Burkholderia thailandensis E264 ...    64   1e-08
BX571966_1516(BX571966|pid:none) Burkholderia pseudomallei strai...    63   2e-08
BA000012_1330(BA000012|pid:none) Mesorhizobium loti MAFF303099 D...    62   3e-08
CP000283_1918(CP000283|pid:none) Rhodopseudomonas palustris BisB...    51   6e-05
CP000480_2717(CP000480|pid:none) Mycobacterium smegmatis str. MC...    49   3e-04
BX640428_188(BX640428|pid:none) Bordetella parapertussis strain ...    48   4e-04

 psg: 0.48  gvh: 0.38  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VSD151 is cyt

>VSD151F.Seq
TCCGCAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAATGTT
TTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATTGAA
GGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGTGAA
ACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAATTA
ATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATTCGT
ATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTAAAG
AAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAAAAA
TTCTTTTCAAAATAAATTAAATTCTCTATTCTTTTAATTTTAAAAAAAAAA---------
-

>VSD151Z.Seq
----------CAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGG
AATGTTTTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATT
ATTGAAGGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACT
GGTGAAACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTAT
GAATTAATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTA
ATTCGTATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGAT
GTAAAGAAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTA
CAAAAATTCTTTTCAAAATAAATTAAATTCTCT

>VSD151P.Seq
TCCGCAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAATGTT
TTACGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATTGAA
GGTGACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGTGAA
ACCAATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAATTA
ATTGAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATTCGT
ATTACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTAAAG
AAGGATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAAAAA
TTCTTTTCAAAATAAATTAAATTCTCTATTCTTTTAATTTTAAAAAAAAAA---------
-CAAGAGTACAAAAATCTGCAATTTTAAATGAAAACATAGACAAAGTATGGAATGTTTTA
CGTAATTTCGATTTCCCATCAAAAATCTTCCCAGTAATCGAAAGTTCAATTATTGAAGGT
GACTCAACACCAACCACTGTTGGAGCCATCCGTGTTTTAAAGTGGAAAACTGGTGAAACC
AATAAACAAAGATTATTAGAACTCTCTGATCTTTCACATAAAATCATTTATGAATTAATT
GAAAGTGAACAAACTGCTGAAGTTACTGCCTACATTAGCACCATTAAATTAATTCGTATT
ACTGAATCAAATCAAACCCTTTTGACTTGGGAAAGTGAATTTTCAGCAGATGTAAAGAAG
GATTTAATTAATTTCGAATCAAAATCAGTTTCCTTAAATTTACAAGATTTACAAAAATTC
TTTTCAAAATAAATTAAATTCTCT