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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD229
Library VS
(Link to library)
Clone ID VSD229
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15350-1
Original site URL
Representative seq. ID VSD229P
(Link to Original site)
Representative DNA sequence 
>VSD229 (VSD229Q) /CSM/VS/VSD2-B/VSD229Q.Seq.d/
GAAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGT
TGGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCC
AACTCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTT
CAATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATAT
TCAAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGT
TCCAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGG
TAACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAG
ATACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATT
CGTCTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACTTCTCGTTCAACAACCAA
CCTTAAAATTACCAGAAACCGTTCTCGATTCCAACTTAATGAGTTTATATGAAAAAGAAG
TCGCTGATGCTGCTGCCCTCCCATTACCAGAAGACAATGATGACTTGTAAACAAATTTAA
AAAAAAAAXXXXXXXXXXGAAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTT
AGTTGGTGATGGTGGTGTTGGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATT
CGAACCAAGATATATCCCAACTCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAA
TTTCGGTAAAATCCACTTCAATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTT
AAGAGATGGTTATTATATTCAAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCG
TATTTCATACAAAAATGTTCCAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACAT
CCCAATCGTTTTATGTGGTAACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCA
AATTGTTTTCCATCGTAGATACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTA
CAATTTCGAAAAACCATTCGTCTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCAC
TCTCGTTCAACAACCAACCTTAAAATTACCAGAAACCGTTCTCGATTCCAACTTAATGAG
TTTATATAAAAAGAAGTCGCTGATGCTGCTGCCCTCCCATTACCAGAAGACAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
kvkmaekeqiklvlvgdggvgkttfvqrhltgefepryiptlgvsvhplifytnfgkihf
nvwdtagqekfgglrdgyyiqgncaiimfdvtsrisyknvpnwhsdltrvcenipivlcg
nkvdvkdrkvkpsqivfhrrynlsyydvsaksnynfekpfvwltskllgnkavtsrsttn
lkitrnrsrfqlnefi*krsr*cccppitrrq**LVNKFKKK---

---KVKMAEKEQIKLVLVGDGGVGKTTFVQRHLTGEFEPRYIPTLGVSVHPLIFYTNFGK
IHFNVWDTAGQEKFGGLRDGYYIQGNCAIIMFDVTSRISYKNVPNWHSDLTRVCENIPIV
LCGNKVDVKDRKVKPSQIVFHRRYNLSYYDVSAKSNYNFEKPFVWLTSKLLGNKAVTLVQ
QPTLKLPETVLDSNLMSLYKKKSLMLLPSHYQKT
Translated Amino Acid sequence (All Frames) 
Frame A:
es*ngrkrtn*islsw*wwcw*nhfcptssyw*irtkiypnswcispsinllhqfr*npl
qcmgyrwsrkirwfkrwllysr*lchyyv*chipyfiqkcsklal*fnqsl*khpnrfmw
*qs*c*rs*sqtipncfps*iqfiil*cfsqiklqfrktirlvniktpr*qgchfsfnnq
p*nyqkpfsipt**vymkkkslmllpshyqktmmtckqi*kk---

---es*ngrkrtn*islsw*wwcw*nhfcptssyw*irtkiypnswcispsinllhqfr*
nplqcmgyrwsrkirwfkrwllysr*lchyyv*chipyfiqkcsklal*fnqsl*khpnr
fmw*qs*c*rs*sqtipncfps*iqfiil*cfsqiklqfrktirlvniktpr*qgchsrs
ttnlkitrnrsrfqlnefi*kevadaaalplped

Frame B:
kvkmaekeqiklvlvgdggvgkttfvqrhltgefepryiptlgvsvhplifytnfgkihf
nvwdtagqekfgglrdgyyiqgncaiimfdvtsrisyknvpnwhsdltrvcenipivlcg
nkvdvkdrkvkpsqivfhrrynlsyydvsaksnynfekpfvwltskllgnkavtsrsttn
lkitrnrsrfqlnefi*krsr*cccppitrrq**LVNKFKKK---

---KVKMAEKEQIKLVLVGDGGVGKTTFVQRHLTGEFEPRYIPTLGVSVHPLIFYTNFGK
IHFNVWDTAGQEKFGGLRDGYYIQGNCAIIMFDVTSRISYKNVPNWHSDLTRVCENIPIV
LCGNKVDVKDRKVKPSQIVFHRRYNLSYYDVSAKSNYNFEKPFVWLTSKLLGNKAVTLVQ
QPTLKLPETVLDSNLMSLYKKKSLMLLPSHYQKT

Frame C:
klkwqkknkln*s*lvmvvlvkpllsnvillvnsnqdisqllvyqsih*sstpisvksts
mygiplvkknsvv*emviifkvivpllclmshpvfhtkmfqigtli*pefvktsqsfyvv
tklmlkivksnhpklfsivdtiyhtmmfqpnqitisknhssg*hqnssvtrlsllvqqpt
lklpetvldsnlmslyekevadaaalplpednddl*tnlkkk---

---klkwqkknkln*s*lvmvvlvkpllsnvillvnsnqdisqllvyqsih*sstpisvk
stsmygiplvkknsvv*emviifkvivpllclmshpvfhtkmfqigtli*pefvktsqsf
yvvtklmlkivksnhpklfsivdtiyhtmmfqpnqitisknhssg*hqnssvtrlslsfn
nqp*nyqkpfsipt**vyikrsr*cccppitrrq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD229 (VSD229Q) /CSM/VS/VSD2-B/VSD229Q.Seq.d/                       1304   0.0
SSI774 (SSI774Q) /CSM/SS/SSI7-D/SSI774Q.Seq.d/                       1289   0.0
SLA506 (SLA506Q) /CSM/SL/SLA5-A/SLA506Q.Seq.d/                       1283   0.0
VSI212 (VSI212Q) /CSM/VS/VSI2-A/VSI212Q.Seq.d/                       1273   0.0
AHG822 (AHG822Q) /CSM/AH/AHG8-A/AHG822Q.Seq.d/                       1241   0.0
VSG423 (VSG423Q) /CSM/VS/VSG4-A/VSG423Q.Seq.d/                       1205   0.0
VSG164 (VSG164Q) /CSM/VS/VSG1-C/VSG164Q.Seq.d/                       1178   0.0
VSI638 (VSI638Q) /CSM/VS/VSI6-B/VSI638Q.Seq.d/                       1156   0.0
VSF607 (VSF607Q) /CSM/VS/VSF6-A/VSF607Q.Seq.d/                       1118   0.0
SLH458 (SLH458Q) /CSM/SL/SLH4-C/SLH458Q.Seq.d/                       1116   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L09720|L09720.1 Dictyostelium discoideum GTP-binding protein (TC4/RAN) mRNA, complete cds.  1170  0.0  2
AX489114|AX489114.1 Sequence 6414 from Patent WO02053728.        115  4e-54  4
AF190700|AF190700.1 Candida albicans small G-protein Gsp1p (GSP1) gene, complete cds.   115  2e-52  3
CA758831|AAF60289.1, mRNA sequence.    74  9e-50  5
BM275423|BM275423.1 PfESToaa68f04.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    92  6e-39  5
BU498261|BU498261.1 PfESToab94h09.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    92  7e-39  5
BM273404|BM273404.1 PfESToaa50c11.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    92  7e-39  5
BI815642|BI815642.1 PfESToaa31a10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    92  7e-39  5
BI815554|BI815554.1 PfESToaa29h11.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    92  7e-39  5
BQ452295|BQ452295.1 PfESToaa94h01.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:RAN_PLAFA P38545 GTP-BINDING NUCLEAR PROTEIN RAN/TC4. ;, mRNA sequence.    92  7e-39  5
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P33519) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   368   e-103
(O17915) RecName: Full=GTP-binding nuclear protein ran-1; AltNam...   275   3e-74
EF103373_1(EF103373|pid:none) Haliotis discus discus Ran-1-prov ...   274   4e-74
(P52301) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   276   1e-73
JC1455(JC1455;S20712)GTP-binding protein Ran/TC4 - dog                279   1e-73
BC041293_1(BC041293|pid:none) Xenopus laevis RAN, member RAS onc...   276   2e-73
CR761555_1(CR761555|pid:none) Xenopus tropicalis finished cDNA, ...   276   2e-73
CP000499_469(CP000499|pid:none) Pichia stipitis CBS 6054 chromos...   271   2e-73
CR382138_83(CR382138|pid:none) Debaryomyces hansenii strain CBS7...   271   2e-73
(P32836) RecName: Full=GTP-binding nuclear protein GSP2/CNR2;  &...   272   2e-73
protein update 2009. 7.29
PSORT 

 psg: 0.44  gvh: 0.35  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VSD229 is cyt
5' end seq. ID VSD229F
5' end seq. 
>VSD229F.Seq
GAAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGT
TGGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCC
AACTCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTT
CAATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATAT
TCAAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGT
TCCAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGG
TAACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAG
ATACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATT
CGTCTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACTTCTCGTTCAACAACCAA
CCTTAAAATTACCAGAAACCGTTCTCGATTCCAACTTAATGAGTTTATATGAAAAAGAAG
TCGCTGATGCTGCTGCCCTCCCATTACCAGAAGACAATGATGACTTGTAAACAAATTTAA
AAAAAAAA----------
Length of 5' end seq. 668
3' end seq. ID VSD229Z
3' end seq. 
>VSD229Z.Seq
----------GAAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTG
ATGGTGGTGTTGGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAA
GATATATCCCAACTCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTA
AAATCCACTTCAATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATG
GTTATTATATTCAAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCAT
ACAAAAATGTTCCAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCG
TTTTATGTGGTAACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTT
TCCATCGTAGATACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCG
AAAAACCATTCGTCTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACTCTCGTTC
AACAACCAACCTTAAAATTACCAGAAACCGTTCTCGATTCCAACTTAATGAGTTTATATA
AAAAGAAGTCGCTGATGCTGCTGCCCTCCCATTACCAGAAGACAA
Length of 3' end seq. 635
Connected seq. ID VSD229P
Connected seq. 
>VSD229P.Seq
GAAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTTAGTTGGTGATGGTGGTGT
TGGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATTCGAACCAAGATATATCCC
AACTCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAATTTCGGTAAAATCCACTT
CAATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTTAAGAGATGGTTATTATAT
TCAAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCGTATTTCATACAAAAATGT
TCCAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACATCCCAATCGTTTTATGTGG
TAACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCAAATTGTTTTCCATCGTAG
ATACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTACAATTTCGAAAAACCATT
CGTCTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCACTTCTCGTTCAACAACCAA
CCTTAAAATTACCAGAAACCGTTCTCGATTCCAACTTAATGAGTTTATATGAAAAAGAAG
TCGCTGATGCTGCTGCCCTCCCATTACCAGAAGACAATGATGACTTGTAAACAAATTTAA
AAAAAAAA----------GAAAGTTAAAATGGCAGAAAAAGAACAAATTAAATTAGTCTT
AGTTGGTGATGGTGGTGTTGGTAAAACCACTTTTGTCCAACGTCATCTTACTGGTGAATT
CGAACCAAGATATATCCCAACTCTTGGTGTATCAGTCCATCCATTAATCTTCTACACCAA
TTTCGGTAAAATCCACTTCAATGTATGGGATACCGCTGGTCAAGAAAAATTCGGTGGTTT
AAGAGATGGTTATTATATTCAAGGTAATTGTGCCATTATTATGTTTGATGTCACATCCCG
TATTTCATACAAAAATGTTCCAAATTGGCACTCTGATTTAACCAGAGTTTGTGAAAACAT
CCCAATCGTTTTATGTGGTAACAAAGTTGATGTTAAAGATCGTAAAGTCAAACCATCCCA
AATTGTTTTCCATCGTAGATACAATTTATCATACTATGATGTTTCAGCCAAATCAAATTA
CAATTTCGAAAAACCATTCGTCTGGTTAACATCAAAACTCCTCGGTAACAAGGCTGTCAC
TCTCGTTCAACAACCAACCTTAAAATTACCAGAAACCGTTCTCGATTCCAACTTAATGAG
TTTATATAAAAAGAAGTCGCTGATGCTGCTGCCCTCCCATTACCAGAAGACAA
Length of connected seq. 1303
Full length Seq ID -
Full length Seq. -
Length of full length seq. -