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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF233
Library VS
(Link to library)
Clone ID VSF233
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15495-1
Original site URL
Representative seq. ID VSF233P
(Link to Original site)
Representative DNA sequence 
>VSF233 (VSF233Q) /CSM/VS/VSF2-B/VSF233Q.Seq.d/
ATGATGATAAAGTCCAACCAGGATCAGCTGCACTTATTGTTGAAAAGTTAGGTGATTACT
TAAGAGATAATGGTTATTAAGATATTAATATCTTGTTAAAACCAATTAGATTTAATTTTA
AGAATTAAAAAAAATAATAATAATAATAAAATTTAAATGTCACTACTAAAATAAATAAAT
AAAAAAAAAAXXXXXXXXXXATGATGATAAAGTCCAACCAGGATCAGCTGCACTTATTGT
TGAAAAGTTAGGTGATTACTTAAGAGATAATGGTTATTAAGATATTAATATCTTGTTAAA
ACCAATTAGATTTAATTTTAAGAATTAAAAAAAATAATAATAATAATAAAATTTAAATGT
CACTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
mmiksnqdqlhlllks*vit*eimvikilisc*nqldlilrikknnnnnki*msllk*IN
KKK---

---DDKVQPGSAALIVEKLGDYLRDNGY*dinillkpirfnfkn*kk*****nlnvt
Translated Amino Acid sequence (All Frames) 
Frame A:
mmiksnqdqlhlllks*vit*eimvikilisc*nqldlilrikknnnnnki*msllk*IN
KKK---

---mmiksnqdqlhlllks*vit*eimvikilisc*nqldlilrikknnnnnki*msl

Frame B:
***sptrisctyc*kvr*llkr*wllry*ylvktn*i*f*elkkiiiiikfkchy*nk*i
kkk---

---***sptrisctyc*kvr*llkr*wllry*ylvktn*i*f*elkkiiiiikfkch

Frame C:
ddkvqpgsaaliveklgdylrdngy*dinillkpirfnfkn*kk*****nlnvttkink*
kk---

---DDKVQPGSAALIVEKLGDYLRDNGY*dinillkpirfnfkn*kk*****nlnvt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH386 (VSH386Q) /CSM/VS/VSH3-D/VSH386Q.Seq.d/                        280   1e-74
VSG766 (VSG766Q) /CSM/VS/VSG7-C/VSG766Q.Seq.d/                        280   1e-74
VSF233 (VSF233Q) /CSM/VS/VSF2-B/VSF233Q.Seq.d/                        280   1e-74
VHN220 (VHN220Q) /CSM/VH/VHN2-A/VHN220Q.Seq.d/                        280   1e-74
VHL739 (VHL739Q) /CSM/VH/VHL7-B/VHL739Q.Seq.d/                        280   1e-74
VHG720 (VHG720Q) /CSM/VH/VHG7-A/VHG720Q.Seq.d/                        280   1e-74
VHA334 (VHA334Q) /CSM/VH/VHA3-B/VHA334Q.Seq.d/                        280   1e-74
SSM730 (SSM730Q) /CSM/SS/SSM7-B/SSM730Q.Seq.d/                        280   1e-74
SSM510 (SSM510Q) /CSM/SS/SSM5-A/SSM510Q.Seq.d/                        280   1e-74
SSK676 (SSK676Q) /CSM/SS/SSK6-D/SSK676Q.Seq.d/                        280   1e-74
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X61581|X61581.1 D.discoideum mRNA for profilin I.                250  4e-74  2
AC020554|AC020554.4 Homo sapiens chromosome X clone RP11-476P22, WORKING DRAFT SEQUENCE, 17 unordered pieces.    42  0.003  5
AC096711|AC096711.2 Homo sapiens BAC clone RP11-1E22 from 4, complete sequence.    46  0.008  2
BX294188|BX294188.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-255G14.    50  0.019  1
AC115496|AC115496.4 Rattus norvegicus clone CH230-57H5, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    50  0.019  1
AC129101|AC129101.4 Rattus norvegicus clone CH230-432M3, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    48  0.075  1
AC120612|AC120612.5 Rattus norvegicus clone CH230-424E3, WORKING DRAFT SEQUENCE, 3 unordered pieces.    48  0.075  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    48  0.075  1
BZ156311|BZ156311.1 CH230-442L15.TJ CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-442L15, DNA sequence.    48  0.075  1
AL935145|AL935145.12 Zebrafish DNA sequence from clone CH211-173B9 in linkage group 20.    44  0.11  2
dna update 2003. 8.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

S13199(S13199)profilin - slime mold (Physarum polycephalum)            40   0.020
(P26200) RecName: Full=Profilin-2; AltName: Full=Profilin II;  &...    38   0.13
AK340025_1(AK340025|pid:none) Acyrthosiphon pisum ACYPI002963 mR...    36   0.49
(Q9STB6) RecName: Full=Profilin-2; AltName: Full=Pollen allergen...    35   1.1
NRL(1ACF) Profilin i - Acanthamoeba castellanii                        35   1.1
C48405(C48405)profilin-IB - Acanthamoeba sp. (tentative sequence)      35   1.1
NRL(2PRF) Profilin ia (nmr, 19 structures) - Acanthamoeba sp.          35   1.1
(P68696) RecName: Full=Profilin-1A; AltName: Full=Profilin IA; A...    35   1.1
(Q95VF7) RecName: Full=Profilin-1B; AltName: Full=Profilin IB; A...    35   1.1
(P25843) RecName: Full=Profilin; AltName: Full=Protein chickadee...    34   1.4
protein update 2009. 7.31
PSORT 

 psg: 0.89  gvh: 0.13  alm: 0.52  top: 0.40  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VSF233 is nuc
5' end seq. ID VSF233F
5' end seq. 
>VSF233F.Seq
ATGATGATAAAGTCCAACCAGGATCAGCTGCACTTATTGTTGAAAAGTTAGGTGATTACT
TAAGAGATAATGGTTATTAAGATATTAATATCTTGTTAAAACCAATTAGATTTAATTTTA
AGAATTAAAAAAAATAATAATAATAATAAAATTTAAATGTCACTACTAAAATAAATAAAT
AAAAAAAAAA----------
Length of 5' end seq. 190
3' end seq. ID VSF233Z
3' end seq. 
>VSF233Z.Seq
----------ATGATGATAAAGTCCAACCAGGATCAGCTGCACTTATTGTTGAAAAGTTA
GGTGATTACTTAAGAGATAATGGTTATTAAGATATTAATATCTTGTTAAAACCAATTAGA
TTTAATTTTAAGAATTAAAAAAAATAATAATAATAATAAAATTTAAATGTCACTA
Length of 3' end seq. 165
Connected seq. ID VSF233P
Connected seq. 
>VSF233P.Seq
ATGATGATAAAGTCCAACCAGGATCAGCTGCACTTATTGTTGAAAAGTTAGGTGATTACT
TAAGAGATAATGGTTATTAAGATATTAATATCTTGTTAAAACCAATTAGATTTAATTTTA
AGAATTAAAAAAAATAATAATAATAATAAAATTTAAATGTCACTACTAAAATAAATAAAT
AAAAAAAAAA----------ATGATGATAAAGTCCAACCAGGATCAGCTGCACTTATTGT
TGAAAAGTTAGGTGATTACTTAAGAGATAATGGTTATTAAGATATTAATATCTTGTTAAA
ACCAATTAGATTTAATTTTAAGAATTAAAAAAAATAATAATAATAATAAAATTTAAATGT
CACTA
Length of connected seq. 355
Full length Seq ID -
Full length Seq. -
Length of full length seq. -