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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSF343
Library VS
(Link to library)
Clone ID VSF343
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U06692-1
Original site URL
Representative seq. ID VSF343P
(Link to Original site)
Representative DNA sequence 
>VSF343 (VSF343Q) /CSM/VS/VSF3-B/VSF343Q.Seq.d/
ATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTC
ATTTATTTTATTTATTTATTTTATTTTATTTATTTATTCAATTTTTAATTTTATTTAAAA
ATATGGAAGAAATTCAACCATCATCATCAAAAGAAACGGAATCTATTAATAGTAATGAAA
ATGATAATGATAATAAAATTAGTACTACACCAGTTATTTCTATATCAACATTTATAAAGA
GTAAAAAAAGTGCATCAACAAGTACATCACCACCGAAATCTGGTTCATTATTATCAATTC
AAAGAACTTTAAAAAATAGTTCAGAAACACAAATTATAAAAGAAAAAAAGTAATAATAAA
AAAAATTTTAAAAAAAAAAXXXXXXXXXXATTTATTTATTTATTTATTTATTTATTTATT
TATTTATTTATTTATTTATTTATTTATTCATTTATTTTATTTATTTATTTTATTTTATTT
ATTTATTCAATTTTTAATTTTATTTAAAAATATGGAAGAAATTCAACCATCATCATCAAA
AGAAACGGAATCTATTAATAGTAATGAAAATGATAATGATAATAAAATTAGTACTACACC
AGTTATTTCTATATCAACATTTATAAAGAGTAAAAAAAGTGCATCAACAAGTACATCACC
ACCGAAATCTGGTTCATTATTATCAATTCAAAGAACTTTAAAAAATAGTTCAGAAACACA
AATTATAAAAGAAAANAAGTAATA
sequence update 2001. 3.22
Translated Amino Acid sequence 
fiylfiylfiylfiylfiysfilfiyfilfiysifnfi*kygrnstiiikrngiy****k
*****n*yytsyfyiniyke*kkcinkyitteiwfiiinsknfkk*FRNTNYKRKKVIIK
KILKKK---

---LFIYLFIYLFIYLFIYLFIHLFYLFILFYLFIQFLILFKNMEEIQPSSSKETESINS
NENDNDNKISTTPVISISTFIKSKKSASTSTSPPKSGSLLSIQRTLKNSSETQIIKEXK*
Translated Amino Acid sequence (All Frames) 
Frame A:
iylfiylfiylfiylfiylfiyfiylfyfiylfnf*fylkiwkkfnhhhqkkrnllivmk
mimiiklvlhqlflyqhl*rvkkvhqqvhhhrnlvhyyqfkel*kivqkhkl*kkksnnk
knfkkk---

---iylfiylfiylfiylfiylfiyfiylfyfiylfnf*fylkiwkkfnhhhqkkrnlli
vmkmimiiklvlhqlflyqhl*rvkkvhqqvhhhrnlvhyyqfkel*kivqkhkl*kkxs
n

Frame B:
fiylfiylfiylfiylfiysfilfiyfilfiysifnfi*kygrnstiiikrngiy****k
*****n*yytsyfyiniyke*kkcinkyitteiwfiiinsknfkk*FRNTNYKRKKVIIK
KILKKK---

---fiylfiylfiylfiylfiysfilfiyfilfiysifnfi*kygrnstiiikrngiy**
**k*****n*yytsyfyiniyke*kkcinkyitteiwfiiinsknfkk*frntnykrkxv
i

Frame C:
lfiylfiylfiylfiylfihlfylfilfylfiqflilfknmeeiqpsssketesinsnen
dndnkisttpvisistfikskksaststsppksgsllsiqrtlknssetqiikekk***k
kf*kk---

---LFIYLFIYLFIYLFIYLFIHLFYLFILFYLFIQFLILFKNMEEIQPSSSKETESINS
NENDNDNKISTTPVISISTFIKSKKSASTSTSPPKSGSLLSIQRTLKNSSETQIIKEXK*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF343 (VSF343Q) /CSM/VS/VSF3-B/VSF343Q.Seq.d/                        424   e-118
VSF305 (VSF305Q) /CSM/VS/VSF3-A/VSF305Q.Seq.d/                        424   e-118
SSE624 (SSE624Q) /CSM/SS/SSE6-A/SSE624Q.Seq.d/                         44   0.003
SSC569 (SSC569Q) /CSM/SS/SSC5-C/SSC569Q.Seq.d/                         44   0.003
SHK253 (SHK253Q) /CSM/SH/SHK2-C/SHK253Q.Seq.d/                         44   0.003
VSF441 (VSF441Q) /CSM/VS/VSF4-B/VSF441Q.Seq.d/                         42   0.012
SSA642 (SSA642Q) /CSM/SS/SSA6-B/SSA642Q.Seq.d/                         42   0.012
FCL-AD11 (FCL-AD11Q) /CSM/FCL/FCL-AD/FCL-AD11Q.Seq.d/                  42   0.012
AFM301 (AFM301Q) /CSM/AF/AFM3-A/AFM301Q.Seq.d/                         42   0.012
VSI720 (VSI720Q) /CSM/VS/VSI7-A/VSI720Q.Seq.d/                         40   0.047
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   248  e-106  8
AZ167232|AZ167232.1 SP_0094_B2_F12_SP6E Strongylocentrotus purpuratus, purple sea urchin, sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=94 Col=24 Row=L, DNA sequence.    44  1e-06  3
T09672|T09672.1 0251m7 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 0251m, DNA sequence.    50  1e-06  2
AA550389|AA550389.1 1537m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 1537m, DNA sequence.    50  4e-06  2
AL457140|AL457140.1 T. brucei sheared genomic DNA clone 72h02, forward sequence.    42  5e-06  2
BX564198|BX564198.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse70c12_p1c.    44  1e-05  2
AE014822|AE014822.1 Plasmodium falciparum 3D7 chromosome 14 section 7 of 13 of the complete sequence.    40  2e-05  8
BH304722|BH304722.1 CH230-85G22.TJ CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-85G22, DNA sequence.    52  2e-04  2
BH424329|BH424329.1 BOGTW87TR BOGT Brassica oleracea genomic clone BOGTW87, DNA sequence.    40  2e-04  2
BZ426365|BZ426365.1 BONPS76TR BO_1.6_2_KB_tot Brassica oleracea genomic clone BONPS76, DNA sequence.    40  2e-04  2
dna update 2003. 8.23
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 470,877,385
protein update 2009. 3.22
PSORT 

 psg: 0.63  gvh: 0.51  alm: 0.20  top: -0.03  tms: 0.13  mit: 0.40  mip: 0.03
 nuc: 0.04  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 24.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: vesicles of secretory system

>> prediction for VSF343 is end
5' end seq. ID VSF343F
5' end seq. 
>VSF343F.Seq
ATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTC
ATTTATTTTATTTATTTATTTTATTTTATTTATTTATTCAATTTTTAATTTTATTTAAAA
ATATGGAAGAAATTCAACCATCATCATCAAAAGAAACGGAATCTATTAATAGTAATGAAA
ATGATAATGATAATAAAATTAGTACTACACCAGTTATTTCTATATCAACATTTATAAAGA
GTAAAAAAAGTGCATCAACAAGTACATCACCACCGAAATCTGGTTCATTATTATCAATTC
AAAGAACTTTAAAAAATAGTTCAGAAACACAAATTATAAAAGAAAAAAAGTAATAATAAA
AAAAATTTTAAAAAAAAAA----------
Length of 5' end seq. 379
3' end seq. ID VSF343Z
3' end seq. 
>VSF343Z.Seq
----------ATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTAT
TTATTTATTCATTTATTTTATTTATTTATTTTATTTTATTTATTTATTCAATTTTTAATT
TTATTTAAAAATATGGAAGAAATTCAACCATCATCATCAAAAGAAACGGAATCTATTAAT
AGTAATGAAAATGATAATGATAATAAAATTAGTACTACACCAGTTATTTCTATATCAACA
TTTATAAAGAGTAAAAAAAGTGCATCAACAAGTACATCACCACCGAAATCTGGTTCATTA
TTATCAATTCAAAGAACTTTAAAAAATAGTTCAGAAACACAAATTATAAAAGAAAANAAG
TAATA
Length of 3' end seq. 355
Connected seq. ID VSF343P
Connected seq. 
>VSF343P.Seq
ATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTC
ATTTATTTTATTTATTTATTTTATTTTATTTATTTATTCAATTTTTAATTTTATTTAAAA
ATATGGAAGAAATTCAACCATCATCATCAAAAGAAACGGAATCTATTAATAGTAATGAAA
ATGATAATGATAATAAAATTAGTACTACACCAGTTATTTCTATATCAACATTTATAAAGA
GTAAAAAAAGTGCATCAACAAGTACATCACCACCGAAATCTGGTTCATTATTATCAATTC
AAAGAACTTTAAAAAATAGTTCAGAAACACAAATTATAAAAGAAAAAAAGTAATAATAAA
AAAAATTTTAAAAAAAAAA----------ATTTATTTATTTATTTATTTATTTATTTATT
TATTTATTTATTTATTTATTTATTTATTCATTTATTTTATTTATTTATTTTATTTTATTT
ATTTATTCAATTTTTAATTTTATTTAAAAATATGGAAGAAATTCAACCATCATCATCAAA
AGAAACGGAATCTATTAATAGTAATGAAAATGATAATGATAATAAAATTAGTACTACACC
AGTTATTTCTATATCAACATTTATAAAGAGTAAAAAAAGTGCATCAACAAGTACATCACC
ACCGAAATCTGGTTCATTATTATCAATTCAAAGAACTTTAAAAAATAGTTCAGAAACACA
AATTATAAAAGAAAANAAGTAATA
Length of connected seq. 734
Full length Seq ID -
Full length Seq. -
Length of full length seq. -