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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI166
Library VS
(Link to library)
Clone ID VSI166
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSI166P
(Link to Original site)
Representative DNA sequence 
>VSI166 (VSI166Q) /CSM/VS/VSI1-C/VSI166Q.Seq.d/
CAAAAACATAAAACAATCTACAAATATCAACATTGCTATTTTTTACAAATTTTTTTTTTA
ACACATTACCACACCAAGTTTTTCTTAATTTTATTTAACACACATACTTTTAAAAAAAAA
AXXXXXXXXXXGGGNNNANAANAANNNNNNNNNNNNAAANNNNNNNNNNNNTTTTTTTNN
NNNTTTTTTTTTTTNNNNNAANCCCCCCCNNTTTTTTTTTTTTTTTTTTTTCCCCCCNCT
TTTTTTAAAAAAAAAAAAAAAAAAANNTTTTNCNTTTTTTTTTTTNAACCCCNNNNCNNT
TTTTTTTTNCCNNAAAAAAAAAAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
QKHKTIYKYQHCYFLQIFFLTHYHTKFFLILFNTHTFKKK---

---GXXXXXXXKXXXXFFXXFFFXXXXPPXFFFFFFPPXFFKKKKKKXFXXFFFXPXXXF
FXPXKKKK
Translated Amino Acid sequence (All Frames) 
Frame A:
QKHKTIYKYQHCYFLQIFFLTHYHTKFFLILFNTHTFKKK---

---gxxxxxxxxxxxxxfxxxfffxxxppxffffffspxff*kkkkkxxxxfffxtpxxf
ffxxkkkk

Frame B:
knikqstniniaifykfff*hittpsfs*fylthillkkk---

---GXXXXXXXKXXXXFFXXFFFXXXXPPXFFFFFFPPXFFKKKKKKXFXXFFFXPXXXF
FXPXKKKK

Frame C:
kt*nnlqistllfftnfffntlphqvflnfi*htyf*kk---

---xxxxxxxxxxxxxffxxfffxxxppxfffffffpplflkkkkkkxfxfffxnpxxxf
fxxkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI166 (VSI166Q) /CSM/VS/VSI1-C/VSI166Q.Seq.d/                        161   8e-39
VSH254 (VSH254Q) /CSM/VS/VSH2-C/VSH254Q.Seq.d/                        137   1e-31
VSC475 (VSC475Q) /CSM/VS/VSC4-D/VSC475Q.Seq.d/                        137   1e-31
VSA753 (VSA753Q) /CSM/VS/VSA7-C/VSA753Q.Seq.d/                        125   4e-28
VFJ887 (VFJ887Q) /CSM/VF/VFJ8-D/VFJ887Q.Seq.d/                         76   4e-13
SLC226 (SLC226Q) /CSM/SL/SLC2-B/SLC226Q.Seq.d/                         36   0.31
SSE507 (SSE507Q) /CSM/SS/SSE5-A/SSE507Q.Seq.d/                         34   1.2
VSE203 (VSE203Q) /CSM/VS/VSE2-A/VSE203Q.Seq.d/                         32   4.9
VSC785 (VSC785Q) /CSM/VS/VSC7-D/VSC785Q.Seq.d/                         32   4.9
SSB890 (SSB890Q) /CSM/SS/SSB8-D/SSB890Q.Seq.d/                         32   4.9
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   103  2e-38  2
AX348772|AX348772.1 Sequence 230 from Patent WO0202807.           46  0.25  1
AX344389|AX344389.1 Sequence 236 from Patent WO0200926.           46  0.25  1
AX251995|AX251995.1 Sequence 256 from Patent WO0168911.           46  0.25  1
AC095805|AC095805.6 Rattus norvegicus clone CH230-9H5, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    44  0.99  1
AC108546|AC108546.6 Rattus norvegicus clone CH230-164L19, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44  0.99  1
Z15133|Z15133.1 C.reinhardtii chloroplast psbH gene for 10 kd phosphoprotein of  photosystem II.    44  0.99  1
L13303|L13303.1 Chlamydomonas reinhardtii chloroplast photosystem II reaction center protein (psbH) and PSII-N protein (psbN) genes, complete cds.    44  0.99  1
Z23260|Z23260.1 C.reinhardtii chloroplast psbH gene for 9.3 kDa phosphoprotein of photosystem II.    44  0.99  1
BH347086|BH347086.1 CH230-54E6.TJ CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-54E6, DNA sequence.    36  1.4  2
dna update 2003. 7.18
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 238,785,643
protein update 2009. 4.19
PSORT 

 psg: 0.93  gvh: 0.39  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.43  mip: 0.00
 nuc: 0.19  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 24.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: extracellular, including cell wall

>> prediction for VSI166 is nuc
5' end seq. ID VSI166F
5' end seq. 
>VSI166F.Seq
CAAAAACATAAAACAATCTACAAATATCAACATTGCTATTTTTTACAAATTTTTTTTTTA
ACACATTACCACACCAAGTTTTTCTTAATTTTATTTAACACACATACTTTTAAAAAAAAA
A----------
Length of 5' end seq. 121
3' end seq. ID VSI166Z
3' end seq. 
>VSI166Z.Seq
----------GGGNNNANAANAANNNNNNNNNNNNAAANNNNNNNNNNNNTTTTTTTNNN
NNTTTTTTTTTTTNNNNNAANCCCCCCCNNTTTTTTTTTTTTTTTTTTTTCCCCCCNCTT
TTTTTAAAAAAAAAAAAAAAAAAANNTTTTNCNTTTTTTTTTTTNAACCCCNNNNCNNTT
TTTTTTTNCCNNAAAAAAAAAAAAAAA
Length of 3' end seq. 197
Connected seq. ID VSI166P
Connected seq. 
>VSI166P.Seq
CAAAAACATAAAACAATCTACAAATATCAACATTGCTATTTTTTACAAATTTTTTTTTTA
ACACATTACCACACCAAGTTTTTCTTAATTTTATTTAACACACATACTTTTAAAAAAAAA
A----------GGGNNNANAANAANNNNNNNNNNNNAAANNNNNNNNNNNNTTTTTTTNN
NNNTTTTTTTTTTTNNNNNAANCCCCCCCNNTTTTTTTTTTTTTTTTTTTTCCCCCCNCT
TTTTTTAAAAAAAAAAAAAAAAAAANNTTTTNCNTTTTTTTTTTTNAACCCCNNNNCNNT
TTTTTTTTNCCNNAAAAAAAAAAAAAAA
Length of connected seq. 318
Full length Seq ID -
Full length Seq. -
Length of full length seq. -