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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ223
Library VS
(Link to library)
Clone ID VSJ223
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14033-1
Original site URL
Representative seq. ID VSJ223P
(Link to Original site)
Representative DNA sequence 
>VSJ223 (VSJ223Q) /CSM/VS/VSJ2-A/VSJ223Q.Seq.d/
ATTCCGGTGAATCTGCTTTCCCATTAGGTGGTTTAGTAACTGTACCAAATACTCGTGATG
CTGCTGATACATTACGTCAATACTTCACCCAATTAAGATTGGAGCTCGGTGTTCGTCTCT
GCCAACGTGTATATGCTGTTGACCCATCAAAAGCCAACAAATGGTGGATTTGCTTCTCAA
AGAGAAAATTCTTAAACAAAGCCTTATAAAAAATCAAACACCATTAGTAAGATAGACTGA
AATTAGTGATCGATCATCACTATTTTTTTAAAAAAAAAACAAAAAAAAAAXXXXXXXXXX
ATTCCAGGTGAATCTGCTTTCCCATTAGGTGGTTTAGTAACTGTACCAAATACTCGTGAT
GCTGCTGATACATTACGTCAATACTTCACCCAATTAAGATTGGAGCTCGGTGTTCGTCTC
NGCCAACGTGTATATGCTGTTGNCCCATCAAAAGCCAACAAATGGTGGATTTGCTTCTCA
AANANAAAATTCTTAAACAAAGCCTTATAAAAAATCAAACACCATTAGTAAAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
sgesafplgglvtvpntrdaadtlrqyftqlrlelgvrlcqrvyavdpskankwwicfsk
rkflnkal*kikhh**DRLKLVIDHHYFFKKKTKKK---

---IPGESAFPLGGLVTVPNTRDAADTLRQYFTQLRLELGVRLXQRVYAVXPSKANKWWI
CFSXXKFLNKAL*kikhh**kk
Translated Amino Acid sequence (All Frames) 
Frame A:
ipvnllsh*vv**lyqilvmllihyvntspn*dwssvfvsanvymllthqkptnggfasq
rens*tkpykksntiskid*n**siitiflkkkqkk---

---IPGESAFPLGGLVTVPNTRDAADTLRQYFTQLRLELGVRLXQRVYAVXPSKANKWWI
CFSXXKFLNKAL*kikhh**kk

Frame B:
fr*icfpirwfsnctkys*cc*yitsilhpikigarcsslptcicc*piksqqmvdlllk
ekilkqsliknqtplvr*teisdrsslff*kknkkk---

---fqvnllsh*vv**lyqilvmllihyvntspn*dwssvfvsanvymllxhqkptnggf
asqxxns*tkpykksntiskk

Frame C:
sgesafplgglvtvpntrdaadtlrqyftqlrlelgvrlcqrvyavdpskankwwicfsk
rkflnkal*kikhh**DRLKLVIDHHYFFKKKTKKK---

---sr*icfpirwfsnctkys*cc*yitsilhpikigarcssxptciccxpiksqqmvdl
llkxkilkqsliknqtplvkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ223 (VSJ223Q) /CSM/VS/VSJ2-A/VSJ223Q.Seq.d/                        533   e-151
VSE113 (VSE113Q) /CSM/VS/VSE1-A/VSE113Q.Seq.d/                        523   e-148
SSK541 (SSK541Q) /CSM/SS/SSK5-B/SSK541Q.Seq.d/                        523   e-148
SSK515 (SSK515Q) /CSM/SS/SSK5-A/SSK515Q.Seq.d/                        523   e-148
SSE489 (SSE489Q) /CSM/SS/SSE4-D/SSE489Q.Seq.d/                        523   e-148
SSA653 (SSA653Q) /CSM/SS/SSA6-C/SSA653Q.Seq.d/                        523   e-148
SSA490 (SSA490Q) /CSM/SS/SSA4-D/SSA490Q.Seq.d/                        523   e-148
SSG655 (SSG655Q) /CSM/SS/SSG6-C/SSG655Q.Seq.d/                        494   e-139
AHD757 (AHD757Q) /CSM/AH/AHD7-C/AHD757Q.Seq.d/                        400   e-111
VSH662 (VSH662Q) /CSM/VS/VSH6-C/VSH662Q.Seq.d/                        260   2e-68
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF095932|AF095932.1 Dictyostelium discoideum p21-Arc (arpF) mRNA, complete cds.   523  e-145  1
AU177261| hypothetical protein ZK381.7 - Caenorhabditis elegans.    46  0.43  1
AF277887|AF277887.1 Sida crystallina clone 67SE cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial gene for mitochondrial product.    36  1.0  2
AF277888|AF277888.1 Sida crystallina clone 68SE cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial gene for mitochondrial product.    36  1.1  2
AC131213|AC131213.4 Homo sapiens 12 BAC RP13-544K12 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    42  1.7  3
AC093217|AC093217.4 Homo sapiens chromosome 5 clone CTD-2120M21, complete sequence.    44  1.7  1
AC026209|AC026209.2 Homo sapiens chromosome 3 clone RP11-665L14 map 3p, WORKING DRAFT SEQUENCE, 35 unordered pieces.    44  1.7  1
AF277884|AF277884.1 Sida crystallina clone 63NA cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial gene for mitochondrial product.    34  3.8  2
AF277883|AF277883.1 Sida crystallina clone 62NA cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial gene for mitochondrial product.    34  4.0  2
BQ733352|BQ733352.1 AGENCOURT_8148796 NICHD XGC Emb4 Xenopus laevis cDNA clone IMAGE:5569881 5', mRNA sequence.    32  6.1  2
dna update 2003. 7.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96624) RecName: Full=Actin-related protein 2/3 complex subunit...   141   1e-32
DQ440234_1(DQ440234|pid:none) Aedes aegypti clone AET-2727 actin...    78   1e-13
EF639101_1(EF639101|pid:none) Triatoma infestans clone tin-652 a...    77   3e-13
BT057931_1(BT057931|pid:none) Salmo salar clone ssal-evd-535-243...    74   3e-12
BT046697_1(BT046697|pid:none) Salmo salar clone ssal-eve-558-251...    72   6e-12
BC157161_1(BC157161|pid:none) Xenopus tropicalis actin related p...    72   6e-12
BT024265_1(BT024265|pid:none) Drosophila melanogaster IP15696 fu...    72   8e-12
AE014297_2166(AE014297|pid:none) Drosophila melanogaster chromos...    72   8e-12
AL844518_4(AL844518|pid:none) Zebrafish DNA sequence from clone ...    72   8e-12
DQ214339_1(DQ214339|pid:none) Taeniopygia guttata clone 0063P002...    72   1e-11
protein update 2009. 3.25
PSORT 

 psg: 0.49  gvh: 0.38  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	 16.0 %: mitochondrial

>> prediction for VSJ223 is cyt
5' end seq. ID VSJ223F
5' end seq. 
>VSJ223F.Seq
ATTCCGGTGAATCTGCTTTCCCATTAGGTGGTTTAGTAACTGTACCAAATACTCGTGATG
CTGCTGATACATTACGTCAATACTTCACCCAATTAAGATTGGAGCTCGGTGTTCGTCTCT
GCCAACGTGTATATGCTGTTGACCCATCAAAAGCCAACAAATGGTGGATTTGCTTCTCAA
AGAGAAAATTCTTAAACAAAGCCTTATAAAAAATCAAACACCATTAGTAAGATAGACTGA
AATTAGTGATCGATCATCACTATTTTTTTAAAAAAAAAACAAAAAAAAAA----------
Length of 5' end seq. 290
3' end seq. ID VSJ223Z
3' end seq. 
>VSJ223Z.Seq
----------ATTCCAGGTGAATCTGCTTTCCCATTAGGTGGTTTAGTAACTGTACCAAA
TACTCGTGATGCTGCTGATACATTACGTCAATACTTCACCCAATTAAGATTGGAGCTCGG
TGTTCGTCTCNGCCAACGTGTATATGCTGTTGNCCCATCAAAAGCCAACAAATGGTGGAT
TTGCTTCTCAAANANAAAATTCTTAAACAAAGCCTTATAAAAAATCAAACACCATTAGTA
AAAAAAA
Length of 3' end seq. 237
Connected seq. ID VSJ223P
Connected seq. 
>VSJ223P.Seq
ATTCCGGTGAATCTGCTTTCCCATTAGGTGGTTTAGTAACTGTACCAAATACTCGTGATG
CTGCTGATACATTACGTCAATACTTCACCCAATTAAGATTGGAGCTCGGTGTTCGTCTCT
GCCAACGTGTATATGCTGTTGACCCATCAAAAGCCAACAAATGGTGGATTTGCTTCTCAA
AGAGAAAATTCTTAAACAAAGCCTTATAAAAAATCAAACACCATTAGTAAGATAGACTGA
AATTAGTGATCGATCATCACTATTTTTTTAAAAAAAAAACAAAAAAAAAA----------
ATTCCAGGTGAATCTGCTTTCCCATTAGGTGGTTTAGTAACTGTACCAAATACTCGTGAT
GCTGCTGATACATTACGTCAATACTTCACCCAATTAAGATTGGAGCTCGGTGTTCGTCTC
NGCCAACGTGTATATGCTGTTGNCCCATCAAAAGCCAACAAATGGTGGATTTGCTTCTCA
AANANAAAATTCTTAAACAAAGCCTTATAAAAAATCAAACACCATTAGTAAAAAAAA
Length of connected seq. 527
Full length Seq ID -
Full length Seq. -
Length of full length seq. -