| SKIP000636 | |
| SKIP ID | SKIP000636 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | umbilical cord |
| Cell Tissue(Ja) | 臍帯(線維芽細胞) |
| Cell Origin | Normal |
| Cell Name 1(En) | HiPS-RIKEN-2F |
| Cell Name 1(Ja) | HiPS-RIKEN-2F |
| Cell Name 2(En) | HPS0014 |
| Cell Name 2(Ja) | HPS0014 |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | Fetus |
| Sex | Male |
| Race(En) | Japanese |
| Race(Ja) | Japanese |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | Human iPS cell line. Parent cell line of RCB0197 HUC-Fm(Normal umbilical cord fibroblast, male) |
| Description(Ja) | 臍帯由来線維芽細胞（RCB0197 HUC-Fm)iPS細胞. |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Retrovirus |
| Transgene | pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | MEF (X-rays:5000R or MMC) 1-1.5x10^(6) cells/100mm dish |
| Medium | DMEM/HamF12(2mM L-Glutamine)+20%KSR+0.1mM NEAA+0.1mM 2-Mercaptoethanol+5ng/ml human bFGF |
| Genome Editing | - |
| CO2 | 3% |
| Mycoplasma | Negative |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | - |
| Pluripotent Markers Assay | - |
| in vitro Differentiation | - |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | - |
| in vivo Differentiation Assay | - |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | - |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | Yes |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Tsuyoshi Fujioka |
| Author Name(Ja) | 藤岡 剛 |
| Author Organization(En) | RIKEN BioResource Center |
| Author Organization(Ja) | 理化学研究所バイオリソースセンター |
| Author Contact Email | cellbank[at]brc[dot]riken[dot]jp |
| PI Organization(En) | RIKEN BioResource Center |
| PI Organization(Ja) | 理化学研究所バイオリソースセンター |
| PI Name(En) | Yukio Nakamura |
| PI Name(Ja) | 中村 幸夫 |
| PI Contact Email | cellbank[at]brc[dot]riken[dot]jp |
| Availability | Available |
| Provider Organization(En) | RIKEN BioResource Center |
| Provider Organization(Ja) | 理化学研究所バイオリソースセンター |
| Provider Email | cellbank[at]brc[dot]riken[dot]jp |
| Provider URL | http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HPS0014&type=1 |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 20973836 |
| DOI | 10.1111/j.1749-0774.2010.00091.x |
| Title | Establishment of induced pluripotent stem cells from human neonatal tissues. |
| Authors | Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y |
| Journal | Hum Cell |
| Year | 2010 |
| Volume | 23 |
| Issue | 3 |
| Pages | 113-8 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/20973836 |
| Free input | - |
| Note | - |