SKIP000690
SKIP ID SKIP000690
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) Arm
Cell Tissue(Ja) Arm
Cell Origin Diseased
Cell Name 1(En) CF-RiPS1.2
Cell Name 1(Ja) CF-RiPS1.2
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) のう胞性線維症
ICD Code 1 E849
Disease Name 1(En) cystic fibrosis
OMIM1 219700
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex --
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) hiPS-cells derived from an adult cystic fibrosis patient .
They were derived using modified mRNAs coding reprogramming factors OCT4, SOX2, KLF4, c-MYC and LIN28 (OSKML) with molar concentrations in the ratio 3:1:1:1:1.
Description(Ja) 成人嚢胞性線維症患者由来iPS細胞。
低酸素下でKlf4, c-Myc, Oct4, Sox2とLIN28を3:1:1:1:1の割合でコードした合成RNAを、線維芽細胞株に20日間毎日添加し、遺伝子を細胞へ導入した。
Cell Morphology human ES-like
Grade Research Grade
Vector Other
Transgene OCT4 , SOX2 , KLF4 , c-MYC and LIN28 (OSKML)
Adhesiveness -
Feeder Yes
Feeder Cell γ -irradiated human neonatal fibroblast feeders (GlobalStem)
Medium DMEM/F12 + 20% KOSR + 5ng/ml FGF+(-)valproic acid (VPA)+a histone deacetylase inhibitor
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunostaining
in vitro Differentiation Yes
in vitro Differentiation Assay embryoid body formation
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay Yes
Other 1 Assay Method qRT-PCR7
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype -
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) -
Author Name(Ja) -
Author Organization(En) -
Author Organization(Ja) -
Author Contact Email -
PI Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Name(En) Derrick J. Rossi
PI Name(Ja) Derrick J. Rossi
PI Contact Email -
Availability Information Only
Provider Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
Provider Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
Provider Email rossi[at]idi[dot]harvard[dot]edu
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 20888316
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21368825
DOI 10.1016/j.stem.2010.08.012
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10.1038/nature09805
Title Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.
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Somatic coding mutations in human induced pluripotent stem cells.
Authors Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ
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Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K
Journal Cell Stem Cell
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Nature
Year 2010
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2011
Volume 7
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471
Issue 5
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7336
Pages 618-30
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63-7
URL http://www.ncbi.nlm.nih.gov/pubmed/20888316
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http://www.ncbi.nlm.nih.gov/pubmed/21368825
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