Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) foreskin
Cell Tissue(Ja) foreskin
Cell Origin Normal
Cell Name 1(En) BJ-RiPS1.2
Cell Name 1(Ja) BJ-RiPS1.2
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range 0-9
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) hiPS-cells establishes from BJ postnatal fibroblasts.
They were derived using modified mRNAs coding reprogramming factors OCT4, SOX2, KLF4, c-MYC and LIN28 (OSKML) with molar concentrations in the ratio 3:1:1:1:1.
Daily transfection with the modified RNA KMOSL cocktail gave rise to numerous hESC-like colonies in BJ cultures that were mechanically picked at day 18, 20, 21, and 25, respectively.
Description(Ja) BJ新生児線維芽細胞由来iPS細胞。
低酸素下でKlf4, c-Myc, Oct4, Sox2とLIN28を3:1:1:1:1の割合でコードした合成RNAを、線維芽細胞株に20日間毎日添加し、遺伝子を細胞へ導入した。
Cell Morphology human ES-like
Grade Research Grade
Vector Other
Transgene OCT4 , SOX2 , KLF4 , c-MYC and LIN28 (OSKML)
Adhesiveness -
Feeder Yes
Feeder Cell γ -irradiated human neonatal fibroblast feeders (GlobalStem)
Medium DMEM/F12 + 20% KOSR + 5ng/ml FGF+(-)valproic acid (VPA)+a histone deacetylase inhibitor
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunostaining
in vitro Differentiation Yes
in vitro Differentiation Assay embryoid body formation
in vivo Differentiation Yes
in vivo Differentiation Assay Teratoma assay
Other 1 Assay Yes
Other 1 Assay Method qRT-PCR
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) -
Author Name(Ja) -
Author Organization(En) -
Author Organization(Ja) -
Author Contact Email -
PI Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Name(En) Derrick J. Rossi
PI Name(Ja) Derrick J. Rossi
PI Contact Email rossi[at]idi[dot]harvard[dot]edu
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 20888316
DOI 10.1016/j.stem.2010.08.012
Title Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.
Somatic coding mutations in human induced pluripotent stem cells.
Authors Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ
Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K
Journal Cell Stem Cell
Year 2010
Volume 7
Issue 5
Pages 618-30
Free input
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