| FBB3 | |
| Project ID |  FBB3 [Protein] |
| Project Theme | 3D structural and functional analyses for elucidation of the mechanism of mitochondrial respiration |
| Project Theme (short) | Mitochondrial respiration |
| Principal Investigator | Shinya Yoshikawa |
| Affiliation | Graduate School of Life Science, University of Hyogo |
| Backgrounds | - Mitochondria are described as cellular power plants of eukaryotic cells - Elucidation of structures and functions of enzymes involved in the energy conversion is fundamental to understand the life of the cell |
| Highlights | - Crystal preparations are in progress towards the structural analysis of the entire NADH dehydrogenase complex - The precise structure of the active center of cytochrome c oxidase has revealed its efficient redox reaction mechanism |
| Outline | Mitochondria are described as cellular power plants because they generate adenosine triphosphate (ATP), used as a source of chemical energy. Their structures are double-membraned composed of inner and outer membranes. Protein complexes in the inner membrane (NADH dehydrogenase, cytochrome c reductase, and cytochrome c oxidase) perform the transfer of electron obtained by glycolysis and the incremental release of energy is used to pump protons (H+) into the intermembrane space. As the proton concentration increases in the intermembrane space, a strong electrochemical gradient is established across the inner membrane. The protons can return to the matrix through the ATP synthase, and their potential energy is used to synthesize ATP. Here we will try to solve the entire structures of NADH dehydrogenase and F1-FoATP synthase. While we already reported the structure of cytochrome c oxidase in 1995, we will refine the structure determination to fully understand the redox process. |
| Review | - |
| CSML File | FBB3.csml |