| FEB5 | |
| Project ID |  FEB5 [Protein] |
| Project Theme | Structure-function analyses and improvement of useful enzymes for chiral compound prodcution |
| Project Theme (short) | Enzymes for chiral compounds |
| Principal Investigator | Sakayu Shimizu |
| Affiliation | Bioenvironmental Science, Kyoto Gakuen University |
| Backgrounds | - Chiral compounds, which have non-superimposable mirror image because of the presence of asymmetric carbon atoms, are in great demand especially for pharmaceutical productions - Among organic syntheses, enzymes or microorganisms employed for the chiral compound production, enzyme utilization is efficient - Based on the structural understanding, we will modify natural enzymes to create novel enzymes |
| Highlights | - We have determined the structures of enzyme-coenzyme complexes for our three target enzymes, stereospecific reductase of C=C bond, threonine aldolases, and carbonyl reductases - Structural information provided insights into stereospecificity of enzymatic reactions - We could modify quinuclidinone oxidoreductase to function with an inexpensive coenzyme based on its structural knowledge |
| Outline | Chiral compounds, which have non-superimposable mirror image because of the presence of asymmetric carbon atoms, are in great demand especially for pharmaceutical productions, since one of chiral molecules, right-handed or left-handed, exhibits the efficacy as a drug in most cases. Organic syntheses, enzymes or microorganisms are employed for the production of chiral compounds. Enzyme utilization is efficient among them. For example, while the synthesis of (R)-3-quinuclidinol, a chiral pharmaceutical intermediate, needs a series of reaction steps in organic synthesis, it can be accomplished in one step with the use of 3-quinuclidinone oxidoreductase. In this project, we will solve structures of enzymes useful for the synthesis of chiral compounds including quinuclidinone oxidoreductase and the complexes with their substrates. Based on the structural understanding, we will modify natural enzymes to create novel enzymes. |
| Review | - |
| CSML File | FEB5.csml |