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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK154
Library AF
(Link to library)
Clone ID AFK154
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16377-1
Original site URL
Representative seq. ID AFK154P
(Link to Original site)
Representative DNA sequence 
>AFK154 (AFK154Q) /CSM/AF/AFK1-C/AFK154Q.Seq.d/
TTTCATTCATCACCCTACAATTTAATTACATACATATATATATATAAACAATAATAAAAA
TGGGTTTCCGTTTCTTAGATATTGTTAAACCATTTACCTCACTCGTTCCAGAGGTTGGTC
AACCAGATAGAAAAATTCCTTTCCGTGAAAAGGTTCTTTGGACAGCAATTTGTTTATTCA
TTTTCTTAGTTTGCAGTCAAATTCCACTTTATGGTATTAGATCAACTGATTCATCAGATC
CATTTTACTGGGCTAAAGTTATTATGGCCTCAAACAGAGGTACTTTAATGGAATTAGGTA
TTTCACCAATCGTTACATCAGGTATGGTTATGCAATTATTAGCAGGTGCCAAATTAATTG
AAATTGATCAATCTGTTAAAGCCGATAGAGATTTATTCTCTGCTGCCCAAAAATTATTTG
GTATGTTAATTTGTGTAGGTCAAGGTGTAGCTTATATTTGGAGTGGTTCATATGGTGATC
CAGCAGTTTTAGGATTTGGTAACTGCTTTTTAATTGTTTTACAATTATTCTTTGCAGGTA
TTATTGTTATGCTTTTGGATGAACTTTTACAAAAAGGATATGGTATTGGATCAGGTATTT
CATTATTTATTGCAACCAXXXXXXXXXXTCAACAAGGTACCTATCCAATCAAGTTATTCT
ACACTTCAAATATTCCAATCATTCTTCAAAGTGCACTCGTTAGCAATCTTTATTTCATCT
CTCAATTATTGNATCGTCGTTTCCCAGATAACATTTTGGTAAACTTATTTGGTGCTTGGA
GAACCAGTGAATATTCACAACAAATGATTCCAGTCTCTGGTCTCACCTATTACATCAGCT
CCCCAAATAATATGAGCGCTGTTTTGGCCGATCCATTCCATGCCCTCTTTTACATCACCT
TTATGTTGACAAGTTGTGCTCTNTTCTCCAAAGTTTGGATCGAAGTTAGTGGTTCCTCTG
CTCGTGACGTTGCTAAGCAACTCAAAGATCAACAAATGACCATGAAAGGTCATCGTGATA
CCTCTGTCATCAAGGAACTCAATCGTTATATTCCAACCGCTGCCGCTTTTGGTGGTCTCT
GTATTGGTGCTCTCACTGTCGTCGCCGATTTCATGGGTGCCATCGGTTCTGGTACCGGTA
TTTTATTAGCTGTCACTATCATCTATCAATACTTTGAAACCTTTGTTAAAGAACAACAAG
AACNTTCNGGNGGTATTGGGGTCTTTTCTAAAATAAAAAAAAAAAAAATTTAATTAAAAA
AAGAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sfitlqfnyihiyi*TIIKMGFRFLDIVKPFTSLVPEVGQPDRKIPFREKVLWTAICLFI
FLVCSQIPLYGIRSTDSSDPFYWAKVIMASNRGTLMELGISPIVTSGMVMQLLAGAKLIE
IDQSVKADRDLFSAAQKLFGMLICVGQGVAYIWSGSYGDPAVLGFGNCFLIVLQLFFAGI
IVMLLDELLQKGYGIGSGISLFIAT---

---QQGTYPIKLFYTSNIPIILQSALVSNLYFISQLLXRRFPDNILVNLFGAWRTSEYSQ
QMIPVSGLTYYISSPNNMSAVLADPFHALFYITFMLTSCALFSKVWIEVSGSSARDVAKQ
LKDQQMTMKGHRDTSVIKELNRYIPTAAAFGGLCIGALTVVADFMGAIGSGTGILLAVTI
IYQYFETFVKEQQEXSGGIGVFSKIKKKKI*lkken
Translated Amino Acid sequence (All Frames) 
Frame A:
fhsspynlityiyiykq**kwvsvs*illnhlphsfqrlvnqiekflsvkrffgqqfvys
fs*favkfhfmvldqlihqihftglkllwpqtevl*wn*vfhqslhqvwlcny*qvpn*l
klinllkpieiysllpknylvc*fv*vkv*lifgvvhmviqqf*dlvtaf*lfynyslqv
lllcfwmnfykkdmvldqvfhyllqp---

---strylsnqvilhfkysnhsskctr*qslfhlsiixssfpr*hfgkliwclenq*ift
tndsslwshllhqlpk*yercfgrsipcpllhhlyvdklcsxlqsldrs*wflcs*rc*a
tqrstndherss*ylchqgtqslysnrcrfwwslywcshcrrrfhgchrfwyryfischy
hlsil*nlc*rttrtfxxywglf*nkkkknlikkrk

Frame B:
fihhpti*lhtyiyinnnkngfpflryc*tiyltrsrgwstr*knsfp*kgsldsnlfih
flslqsnstlwy*in*firsillg*syyglkqryfngiryftnryirygyaiisrcqin*
n*sic*sr*rfilccpkiiwyvnlcrsrcslylewfiw*sssfriw*llfncftiilcry
ycyafg*tftkriwywiryfiiycn---

---QQGTYPIKLFYTSNIPIILQSALVSNLYFISQLLXRRFPDNILVNLFGAWRTSEYSQ
QMIPVSGLTYYISSPNNMSAVLADPFHALFYITFMLTSCALFSKVWIEVSGSSARDVAKQ
LKDQQMTMKGHRDTSVIKELNRYIPTAAAFGGLCIGALTVVADFMGAIGSGTGILLAVTI
IYQYFETFVKEQQEXSGGIGVFSKIKKKKI*lkken

Frame C:
sfitlqfnyihiyi*TIIKMGFRFLDIVKPFTSLVPEVGQPDRKIPFREKVLWTAICLFI
FLVCSQIPLYGIRSTDSSDPFYWAKVIMASNRGTLMELGISPIVTSGMVMQLLAGAKLIE
IDQSVKADRDLFSAAQKLFGMLICVGQGVAYIWSGSYGDPAVLGFGNCFLIVLQLFFAGI
IVMLLDELLQKGYGIGSGISLFIAT---

---nkvpiqssystlqifqsffkvhslaifisslnyxivvsqitfw*tylvlgepvnihn
k*fqslvspitsapqii*alfwpihsmpsftsplc*qvvlxspkfgsklvvpllvtllsn
skink*p*kviviplssrnsivifqplpllvvsvlvlslsspiswvpsvlvpvfy*lsls
sintlkpllknnknxxxvlgsflk*kkkkfn*kkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK154 (AFK154Q) /CSM/AF/AFK1-C/AFK154Q.Seq.d/                       2256   0.0
AFD243 (AFD243Q) /CSM/AF/AFD2-B/AFD243Q.Seq.d/                       1148   0.0
AFL792 (AFL792Q) /CSM/AF/AFL7-D/AFL792Q.Seq.d/                       1142   0.0
VSH673 (VSH673Q) /CSM/VS/VSH6-D/VSH673Q.Seq.d/                       1140   0.0
AFI657 (AFI657Q) /CSM/AF/AFI6-C/AFI657Q.Seq.d/                       1140   0.0
VFH420 (VFH420Q) /CSM/VF/VFH4-A/VFH420Q.Seq.d/                       1134   0.0
AFO294 (AFO294Q) /CSM/AF/AFO2-D/AFO294Q.Seq.d/                       1134   0.0
AFN556 (AFN556Q) /CSM/AF/AFN5-C/AFN556Q.Seq.d/                       1134   0.0
AFL633 (AFL633Q) /CSM/AF/AFL6-B/AFL633Q.Seq.d/                       1134   0.0
AFK145 (AFK145Q) /CSM/AF/AFK1-B/AFK145Q.Seq.d/                       1134   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL668163|AL668163.1 Ciona intestinalis; larval mRNA; EST 5PRIM end of clone 030ZH11 of  library directional larval cDNA library from Ciona intestinalis  (Ciona).    76  3e-26  3
AV996249|AV996249.1 Ciona intestinalis cDNA, clone:citb43n15, 5' end, single read.    62  1e-22  3
AV968324|AV968324.1 Ciona intestinalis cDNA, clone:cilv15f18, 5' end, single read.    76  2e-22  3
BW291712|BW291712.1 Ciona intestinalis cDNA, clone:cigd045e21, 5' end, single read.    60  3e-21  3
BW049609|BW049609.1 Ciona intestinalis cDNA, clone:cibd068a08, 5' end, single read.    60  3e-21  3
BW023354|BW023354.1 Ciona intestinalis cDNA, clone:rcibd075j10, 3' end, single read.    76  1e-20  2
AV994602|AV994602.1 Ciona intestinalis cDNA, clone:citb38g19, 5' end, single read.    76  2e-20  2
BW224512|BW224512.1 Ciona intestinalis cDNA, clone:cilv058e15, 5' end, single read.    76  2e-20  2
AV985751|AV985751.1 Ciona intestinalis cDNA, clone:cilv37l24, 5' end, single read.    76  2e-20  2
AV838365|AV838365.1 Ciona intestinalis cDNA, clone:rcieg03f05, 3' end, single read.    76  2e-20  2
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK017615_1(AK017615|pid:none) Mus musculus 8 days embryo whole b...   465   e-129
(Q54XK2) RecName: Full=Protein transport protein Sec61 subunit a...   387   e-106
AC004481_8(AC004481|pid:none) Arabidopsis thaliana chromosome 2 ...   302   2e-80
EF676421_1(EF676421|pid:none) Picea sitchensis clone WS02736_J13...   301   2e-80
AK100597_1(AK100597|pid:none) Oryza sativa Japonica Group cDNA c...   300   7e-80
EF147330_1(EF147330|pid:none) Populus trichocarpa clone WS0122_K...   299   1e-79
BT055508_1(BT055508|pid:none) Zea mays full-length cDNA clone ZM...   299   2e-79
AY087495_1(AY087495|pid:none) Arabidopsis thaliana clone 36037 m...   296   8e-79
AY044237_1(AY044237|pid:none) Hordeum vulgare Sec61 alpha subuni...   295   3e-78
AF161718_1(AF161718|pid:none) Triticum aestivum Sec61p (sec61) m...   295   3e-78
protein update 2009. 4.24
PSORT 

 psg: 0.81  gvh: 0.68  alm: 0.15  top: 0.47  tms: 0.47  mit: 0.31  mip: 0.03
 nuc: 0.04  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: plasma membrane
	 36.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic

>> prediction for AFK154 is pla
5' end seq. ID AFK154F
5' end seq. 
>AFK154F.Seq
TTTCATTCATCACCCTACAATTTAATTACATACATATATATATATAAACAATAATAAAAA
TGGGTTTCCGTTTCTTAGATATTGTTAAACCATTTACCTCACTCGTTCCAGAGGTTGGTC
AACCAGATAGAAAAATTCCTTTCCGTGAAAAGGTTCTTTGGACAGCAATTTGTTTATTCA
TTTTCTTAGTTTGCAGTCAAATTCCACTTTATGGTATTAGATCAACTGATTCATCAGATC
CATTTTACTGGGCTAAAGTTATTATGGCCTCAAACAGAGGTACTTTAATGGAATTAGGTA
TTTCACCAATCGTTACATCAGGTATGGTTATGCAATTATTAGCAGGTGCCAAATTAATTG
AAATTGATCAATCTGTTAAAGCCGATAGAGATTTATTCTCTGCTGCCCAAAAATTATTTG
GTATGTTAATTTGTGTAGGTCAAGGTGTAGCTTATATTTGGAGTGGTTCATATGGTGATC
CAGCAGTTTTAGGATTTGGTAACTGCTTTTTAATTGTTTTACAATTATTCTTTGCAGGTA
TTATTGTTATGCTTTTGGATGAACTTTTACAAAAAGGATATGGTATTGGATCAGGTATTT
CATTATTTATTGCAACCA----------
Length of 5' end seq. 618
3' end seq. ID AFK154Z
3' end seq. 
>AFK154Z.Seq
----------TCAACAAGGTACCTATCCAATCAAGTTATTCTACACTTCAAATATTCCAA
TCATTCTTCAAAGTGCACTCGTTAGCAATCTTTATTTCATCTCTCAATTATTGNATCGTC
GTTTCCCAGATAACATTTTGGTAAACTTATTTGGTGCTTGGAGAACCAGTGAATATTCAC
AACAAATGATTCCAGTCTCTGGTCTCACCTATTACATCAGCTCCCCAAATAATATGAGCG
CTGTTTTGGCCGATCCATTCCATGCCCTCTTTTACATCACCTTTATGTTGACAAGTTGTG
CTCTNTTCTCCAAAGTTTGGATCGAAGTTAGTGGTTCCTCTGCTCGTGACGTTGCTAAGC
AACTCAAAGATCAACAAATGACCATGAAAGGTCATCGTGATACCTCTGTCATCAAGGAAC
TCAATCGTTATATTCCAACCGCTGCCGCTTTTGGTGGTCTCTGTATTGGTGCTCTCACTG
TCGTCGCCGATTTCATGGGTGCCATCGGTTCTGGTACCGGTATTTTATTAGCTGTCACTA
TCATCTATCAATACTTTGAAACCTTTGTTAAAGAACAACAAGAACNTTCNGGNGGTATTG
GGGTCTTTTCTAAAATAAAAAAAAAAAAAATTTAATTAAAAAAAGAAAAT
Length of 3' end seq. 640
Connected seq. ID AFK154P
Connected seq. 
>AFK154P.Seq
TTTCATTCATCACCCTACAATTTAATTACATACATATATATATATAAACAATAATAAAAA
TGGGTTTCCGTTTCTTAGATATTGTTAAACCATTTACCTCACTCGTTCCAGAGGTTGGTC
AACCAGATAGAAAAATTCCTTTCCGTGAAAAGGTTCTTTGGACAGCAATTTGTTTATTCA
TTTTCTTAGTTTGCAGTCAAATTCCACTTTATGGTATTAGATCAACTGATTCATCAGATC
CATTTTACTGGGCTAAAGTTATTATGGCCTCAAACAGAGGTACTTTAATGGAATTAGGTA
TTTCACCAATCGTTACATCAGGTATGGTTATGCAATTATTAGCAGGTGCCAAATTAATTG
AAATTGATCAATCTGTTAAAGCCGATAGAGATTTATTCTCTGCTGCCCAAAAATTATTTG
GTATGTTAATTTGTGTAGGTCAAGGTGTAGCTTATATTTGGAGTGGTTCATATGGTGATC
CAGCAGTTTTAGGATTTGGTAACTGCTTTTTAATTGTTTTACAATTATTCTTTGCAGGTA
TTATTGTTATGCTTTTGGATGAACTTTTACAAAAAGGATATGGTATTGGATCAGGTATTT
CATTATTTATTGCAACCA----------TCAACAAGGTACCTATCCAATCAAGTTATTCT
ACACTTCAAATATTCCAATCATTCTTCAAAGTGCACTCGTTAGCAATCTTTATTTCATCT
CTCAATTATTGNATCGTCGTTTCCCAGATAACATTTTGGTAAACTTATTTGGTGCTTGGA
GAACCAGTGAATATTCACAACAAATGATTCCAGTCTCTGGTCTCACCTATTACATCAGCT
CCCCAAATAATATGAGCGCTGTTTTGGCCGATCCATTCCATGCCCTCTTTTACATCACCT
TTATGTTGACAAGTTGTGCTCTNTTCTCCAAAGTTTGGATCGAAGTTAGTGGTTCCTCTG
CTCGTGACGTTGCTAAGCAACTCAAAGATCAACAAATGACCATGAAAGGTCATCGTGATA
CCTCTGTCATCAAGGAACTCAATCGTTATATTCCAACCGCTGCCGCTTTTGGTGGTCTCT
GTATTGGTGCTCTCACTGTCGTCGCCGATTTCATGGGTGCCATCGGTTCTGGTACCGGTA
TTTTATTAGCTGTCACTATCATCTATCAATACTTTGAAACCTTTGTTAAAGAACAACAAG
AACNTTCNGGNGGTATTGGGGTCTTTTCTAAAATAAAAAAAAAAAAAATTTAATTAAAAA
AAGAAAAT
Length of connected seq. 1258
Full length Seq ID -
Full length Seq. -
Length of full length seq. -