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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFD327
Library VF
(Link to library)
Clone ID VFD327
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15434-1
Original site URL
Representative seq. ID VFD327P
(Link to Original site)
Representative DNA sequence 
>VFD327 (VFD327Q) /CSM/VF/VFD3-B/VFD327Q.Seq.d/
AAGCGTTTAAACATACACACTTAAGAGAATGACTAAATTACACTACAAAGTTAAAGATAT
TTCACTTGCCGCTTGGGGTCGTAAGGAAATTGAAATTGCCGAAAATGAAATGCCAGGTTT
AATGACCTTAAGAAAGAAATATGGTCCAGCTCAAATCTTAAAAGGTGCTCGTATTGCAGG
TTGTTTACACATGACTATCCAAACCGCCGTTTTAATTGAAACTTTAACTGCTCTCGGTGC
TCAAGTCCAATGGTCATCATGTTACATTTTCTCCACTCAAGATCAAGCCGCCGCTGCCAT
CGCTGCCACTGGTGTCCCAGTCTATGCCTGGAAAGGTGAAACCGAAGAAGAATACAACTG
GTGTGTCGAACAAACCATTGTTTTCCAAGATGGTCAACCATTAAATATGATCTTAGATGA
TGGTGGTGATTTAACCAACCTCGTCCACGAGAAATACCCACAATTCTTAGCTGGTATCAA
AGGTATCTCTGAAGAAACCACCACTGGTGTCCACAACCTCTACAAAATGTTCAXXXXXXX
XXXTTGCCGTCGTCGCTGGTTACGGTGATGTAGGTAAAGGTTGTGCTCAATCATTATCAA
AAATGGGTGCTCGTGTTTTAGTCACTGAAATCGATCCAATCAATGCCCTCCAAGCCTGTA
TGGATGGTTACCAAATCGTCACCATGGAAACCGCCGCTCCATTATCAAACATTTTCGTCA
CCACCACCGGTTGTCGTGATATCGTCAGAGGTGAACACTTTGCCGTCATGAAAGAAGATG
CCATCGTTTGTAACATTGGTCACTTTGATTGTGAAATCGATGTCGCTTGGTTAAACGCCA
ACGCCAAAAAAGATACCGTCAAACCACAAGTTGACCGTTACACCCTTGCCAACGGTGTCC
ACATCATCCTCTTAGCTGAAGGTCGTCTCGTCAATTTAGGTTGTGGTACTGGTCATCCAT
CTTTTGTTATGTCAAACTCTTTCTGTAACCAAACTTTAGCTCAAATCGCCCTCTGGACTA
AAACTGAAGAATACCCATTAGGTGTCCACTTCTTACCAAAGGTTTTAGATGAAGAAGTTG
CTCGTTTACATTTAGATCAATTAGGTGCTAAATTAACTACCCTCACTGAAAAACAATCCG
AATATTTATCAGTTCCAGTCGCAGGTCCATACAAAGTNATCACTACAGAATAAAATCTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sv*TYTLKRMTKLHYKVKDISLAAWGRKEIEIAENEMPGLMTLRKKYGPAQILKGARIAG
CLHMTIQTAVLIETLTALGAQVQWSSCYIFSTQDQAAAAIAATGVPVYAWKGETEEEYNW
CVEQTIVFQDGQPLNMILDDGGDLTNLVHEKYPQFLAGIKGISEETTTGVHNLYKMF---

---AVVAGYGDVGKGCAQSLSKMGARVLVTEIDPINALQACMDGYQIVTMETAAPLSNIF
VTTTGCRDIVRGEHFAVMKEDAIVCNIGHFDCEIDVAWLNANAKKDTVKPQVDRYTLANG
VHIILLAEGRLVNLGCGTGHPSFVMSNSFCNQTLAQIALWTKTEEYPLGVHFLPKVLDEE
VARLHLDQLGAKLTTLTEKQSEYLSVPVAGPYKVITTE*nl
Translated Amino Acid sequence (All Frames) 
Frame A:
krlniht*end*itlqs*ryftcrlgs*gn*ncrk*narfndlkkeiwsssnlkrcsycr
lfthdypnrrfn*nfncsrcsspmvimlhflhsrssrrchrchwcpslcler*nrrriql
vcrtnhcfprwstikydlr*ww*fnqprpreiptilswyqryl*rnhhwcpqplqnv---

---lpsslvtvm*vkvvlnhyqkwvlvf*slksiqsmpskpvwmvtksspwkpplhyqtf
sspppvvvissevntlps*kkmpsfvtlvtlivksmslg*tptpkkipsnhkltvtplpt
vstsss*lkvvssi*vvvlvihlllcqtlsvtkl*lkspsglklknth*vstsyqrf*mk
kllvyi*in*vln*lpslknnpniyqfqsqvhtkxslqnkif

Frame B:
sv*TYTLKRMTKLHYKVKDISLAAWGRKEIEIAENEMPGLMTLRKKYGPAQILKGARIAG
CLHMTIQTAVLIETLTALGAQVQWSSCYIFSTQDQAAAAIAATGVPVYAWKGETEEEYNW
CVEQTIVFQDGQPLNMILDDGGDLTNLVHEKYPQFLAGIKGISEETTTGVHNLYKMF---

---crrrwlr*cr*rlcsiiikngcscfsh*nrsnqcppslygwlpnrhhgnrrsiikhf
rhhhrls*yrqr*tlcrherrchrl*hwsl*l*nrcrlvkrqrqkryrqtts*plhpcqr
cphhpls*rssrqfrlwywssifcyvklfl*pnfssnrpld*n*ripircplltkgfr*r
scsftfrsirc*inyph*ktirifisssrrsiqsxhyriks

Frame C:
afkhthlre*lnyttklkifhlplgvvrklklpkmkcqv**p*ernmvqlks*kvlvlqv
vyt*lskppf*lkl*llsvlksnghhvtfsplkikpplpslplvsqsmpgkvkpkknttg
vsnkplfskmvnh*i*s*mmvvi*ptsstrnthns*lvskvslkkpplvsttstkcs---

---AVVAGYGDVGKGCAQSLSKMGARVLVTEIDPINALQACMDGYQIVTMETAAPLSNIF
VTTTGCRDIVRGEHFAVMKEDAIVCNIGHFDCEIDVAWLNANAKKDTVKPQVDRYTLANG
VHIILLAEGRLVNLGCGTGHPSFVMSNSFCNQTLAQIALWTKTEEYPLGVHFLPKVLDEE
VARLHLDQLGAKLTTLTEKQSEYLSVPVAGPYKVITTE*nl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD327 (VFD327Q) /CSM/VF/VFD3-B/VFD327Q.Seq.d/                       2313   0.0
VFM405 (VFM405Q) /CSM/VF/VFM4-A/VFM405Q.Seq.d/                       1281   0.0
VFB780 (VFB780Q) /CSM/VF/VFB7-D/VFB780Q.Seq.d/                       1281   0.0
SFH192 (SFH192Q) /CSM/SF/SFH1-D/SFH192Q.Seq.d/                       1275   0.0
VFE338 (VFE338Q) /CSM/VF/VFE3-B/VFE338Q.Seq.d/                       1273   0.0
VFC827 (VFC827Q) /CSM/VF/VFC8-B/VFC827Q.Seq.d/                       1273   0.0
VFB638 (VFB638Q) /CSM/VF/VFB6-B/VFB638Q.Seq.d/                       1273   0.0
SFG465 (SFG465Q) /CSM/SF/SFG4-C/SFG465Q.Seq.d/                       1273   0.0
VFI402 (VFI402Q) /CSM/VF/VFI4-A/VFI402Q.Seq.d/                       1267   0.0
VFE247 (VFE247Q) /CSM/VF/VFE2-B/VFE247Q.Seq.d/                       1267   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M19937|M19937.1 Slime mold (D.discoideum) S-adenosyl-L-homocysteine hydrolase mRNA, complete cds.  1233  0.0  3
X12523|X12523.1 D. discoideum mRNA fragment for S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1).   513  0.0  4
BE580064|BE580064.1 kq36f02.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:K02F2.2 CE17154 S-ADENOSYLHOMOCYSTEINE HYDROLASE ;, mRNA sequence.   125  6e-48  5
BE580043|BE580043.1 kq36c04.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:K02F2.2 CE17154 S-ADENOSYLHOMOCYSTEINE HYDROLASE ;, mRNA sequence.   125  1e-47  5
BF015319|BF015319.1 kq62e12.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to SW:SAHH_CAEEL P27604 ADENOSYLHOMOCYSTEINASE ;, mRNA sequence.   125  7e-46  4
BG227111|BG227111.1 kp97b11.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:K02F2.2 CE17154 S-ADENOSYLHOMOCYSTEINE HYDROLASE ;, mRNA sequence.   125  1e-45  4
AB004537|AB004537.1 Schizosaccharomyces pombe 37 kb genomic DNA, clone c213.    64  1e-45  8
AL022072|AL022072.1 S.pombe chromosome II cosmid c8D2.            64  4e-45  8
U18796|U18796.1 Saccharomyces cerevisiae chromosome V cosmids 9379, 9581, and lambda clone 4678.    68  7e-43  8
AL399016|AL399016.1 T7 end of clone AS0AA012E07 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    66  8e-41  8
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27655(A27655;S06394) adenosylhomocysteinase (EC 3.3.1.1) - slim...   420   e-116
PDBN(1A7A)A MOL_ID: 1;MOL_ID: 1; MOLECULE: S-ADENOSYLHOMOCYSTEIN...   180   4e-91
X12523_1(X12523|pid:none) D. discoideum mRNA fragment for S-aden...   300   7e-85
AY278950_1(AY278950|pid:none) Branchiostoma belcheri tsingtaunes...   316   9e-85
(P27604) RecName: Full=Adenosylhomocysteinase;          Short=Ad...   314   4e-84
(O13639) RecName: Full=Adenosylhomocysteinase;          Short=Ad...   314   4e-84
DQ167408_1(DQ167408|pid:none) Volvariella volvacea S-adenosyl-L-...   310   6e-83
DQ440384_1(DQ440384|pid:none) Aedes aegypti clone AET-5159 S-ade...   306   1e-81
FN357350_26(FN357350|pid:none) Schistosoma mansoni genome sequen...   306   1e-81
BC135905_1(BC135905|pid:none) Xenopus tropicalis S-adenosylhomoc...   305   2e-81
protein update 2009. 6.18
PSORT 

 psg: 0.53  gvh: 0.24  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for VFD327 is nuc
5' end seq. ID VFD327F
5' end seq. 
>VFD327F.Seq
AAGCGTTTAAACATACACACTTAAGAGAATGACTAAATTACACTACAAAGTTAAAGATAT
TTCACTTGCCGCTTGGGGTCGTAAGGAAATTGAAATTGCCGAAAATGAAATGCCAGGTTT
AATGACCTTAAGAAAGAAATATGGTCCAGCTCAAATCTTAAAAGGTGCTCGTATTGCAGG
TTGTTTACACATGACTATCCAAACCGCCGTTTTAATTGAAACTTTAACTGCTCTCGGTGC
TCAAGTCCAATGGTCATCATGTTACATTTTCTCCACTCAAGATCAAGCCGCCGCTGCCAT
CGCTGCCACTGGTGTCCCAGTCTATGCCTGGAAAGGTGAAACCGAAGAAGAATACAACTG
GTGTGTCGAACAAACCATTGTTTTCCAAGATGGTCAACCATTAAATATGATCTTAGATGA
TGGTGGTGATTTAACCAACCTCGTCCACGAGAAATACCCACAATTCTTAGCTGGTATCAA
AGGTATCTCTGAAGAAACCACCACTGGTGTCCACAACCTCTACAAAATGTTCA-------
---
Length of 5' end seq. 533
3' end seq. ID VFD327Z
3' end seq. 
>VFD327Z.Seq
----------TTGCCGTCGTCGCTGGTTACGGTGATGTAGGTAAAGGTTGTGCTCAATCA
TTATCAAAAATGGGTGCTCGTGTTTTAGTCACTGAAATCGATCCAATCAATGCCCTCCAA
GCCTGTATGGATGGTTACCAAATCGTCACCATGGAAACCGCCGCTCCATTATCAAACATT
TTCGTCACCACCACCGGTTGTCGTGATATCGTCAGAGGTGAACACTTTGCCGTCATGAAA
GAAGATGCCATCGTTTGTAACATTGGTCACTTTGATTGTGAAATCGATGTCGCTTGGTTA
AACGCCAACGCCAAAAAAGATACCGTCAAACCACAAGTTGACCGTTACACCCTTGCCAAC
GGTGTCCACATCATCCTCTTAGCTGAAGGTCGTCTCGTCAATTTAGGTTGTGGTACTGGT
CATCCATCTTTTGTTATGTCAAACTCTTTCTGTAACCAAACTTTAGCTCAAATCGCCCTC
TGGACTAAAACTGAAGAATACCCATTAGGTGTCCACTTCTTACCAAAGGTTTTAGATGAA
GAAGTTGCTCGTTTACATTTAGATCAATTAGGTGCTAAATTAACTACCCTCACTGAAAAA
CAATCCGAATATTTATCAGTTCCAGTCGCAGGTCCATACAAAGTNATCACTACAGAATAA
AATCTTT
Length of 3' end seq. 657
Connected seq. ID VFD327P
Connected seq. 
>VFD327P.Seq
AAGCGTTTAAACATACACACTTAAGAGAATGACTAAATTACACTACAAAGTTAAAGATAT
TTCACTTGCCGCTTGGGGTCGTAAGGAAATTGAAATTGCCGAAAATGAAATGCCAGGTTT
AATGACCTTAAGAAAGAAATATGGTCCAGCTCAAATCTTAAAAGGTGCTCGTATTGCAGG
TTGTTTACACATGACTATCCAAACCGCCGTTTTAATTGAAACTTTAACTGCTCTCGGTGC
TCAAGTCCAATGGTCATCATGTTACATTTTCTCCACTCAAGATCAAGCCGCCGCTGCCAT
CGCTGCCACTGGTGTCCCAGTCTATGCCTGGAAAGGTGAAACCGAAGAAGAATACAACTG
GTGTGTCGAACAAACCATTGTTTTCCAAGATGGTCAACCATTAAATATGATCTTAGATGA
TGGTGGTGATTTAACCAACCTCGTCCACGAGAAATACCCACAATTCTTAGCTGGTATCAA
AGGTATCTCTGAAGAAACCACCACTGGTGTCCACAACCTCTACAAAATGTTCA-------
---TTGCCGTCGTCGCTGGTTACGGTGATGTAGGTAAAGGTTGTGCTCAATCATTATCAA
AAATGGGTGCTCGTGTTTTAGTCACTGAAATCGATCCAATCAATGCCCTCCAAGCCTGTA
TGGATGGTTACCAAATCGTCACCATGGAAACCGCCGCTCCATTATCAAACATTTTCGTCA
CCACCACCGGTTGTCGTGATATCGTCAGAGGTGAACACTTTGCCGTCATGAAAGAAGATG
CCATCGTTTGTAACATTGGTCACTTTGATTGTGAAATCGATGTCGCTTGGTTAAACGCCA
ACGCCAAAAAAGATACCGTCAAACCACAAGTTGACCGTTACACCCTTGCCAACGGTGTCC
ACATCATCCTCTTAGCTGAAGGTCGTCTCGTCAATTTAGGTTGTGGTACTGGTCATCCAT
CTTTTGTTATGTCAAACTCTTTCTGTAACCAAACTTTAGCTCAAATCGCCCTCTGGACTA
AAACTGAAGAATACCCATTAGGTGTCCACTTCTTACCAAAGGTTTTAGATGAAGAAGTTG
CTCGTTTACATTTAGATCAATTAGGTGCTAAATTAACTACCCTCACTGAAAAACAATCCG
AATATTTATCAGTTCCAGTCGCAGGTCCATACAAAGTNATCACTACAGAATAAAATCTTT
Length of connected seq. 1190
Full length Seq ID -
Full length Seq. -
Length of full length seq. -