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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI557
Library VF
(Link to library)
Clone ID VFI557
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFI557E
(Link to Original site)
Representative DNA sequence 
>VFI557 (VFI557Q) /CSM/VF/VFI5-C/VFI557Q.Seq.d/
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGCAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGA
AGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGA
AATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAAT
GCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGT
CATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTT
AGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGT
TGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCC
AGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAAT
TAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGC
TTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCC
ATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
sequence update 2001.11.24
Translated Amino Acid sequence 
lnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvw
vkkthmqvmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lk
ph*iqrptekk*PKLCLKPSTPQPCTSPFKPSYPYMPLVVPPGIVMDSGDGVSHTVPIYE
GYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEM
QTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDV
DIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILA
SLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikkqi
Translated Amino Acid sequence (All Frames) 
Frame A:
t*fkyiyinhlkmdgedvqalvidngsgmckagfagddapravfpsivgrprhtgvmvgm
gqkdsyagdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelrvapeehpvllte
aplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgycygfr*wclphctnl*
rlcfttchltfrfsws*syrlhdenln*twllihhhcrkrnrqryqrkislrrprl*s*n
anccliisprkiiritrrsshhhr**tfplprsplptiilrygicwyprnhlqlhhem*c
*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiyls
ftlnfptnvdlqrrirrirsincsqkmflnylinnkktn


Frame B:
lnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvw
vkkthmqvmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lk
ph*iqrptekk*PKLCLKPSTPQPCTSPFKPSYPYMPLVVPPGIVMDSGDGVSHTVPIYE
GYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEM
QTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDV
DIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILA
SLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikkqi


Frame C:
liqiyiyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygryg
skrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*s
pikskgqqrkndpnyv*nlqhpshvrrhssrliliclwsyhrvllwiqvmvsptlyqsmk
vmlyhmpsyv*i*lvvilpit**ks*lnvvthspplpkeksseiskkn*ptspstlklkc
kllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnllvstkppttps*nvml
isvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*l
hsqlsnkcgspkknttnqvhqlftenvsklfnk**knkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI557 (VFI557Q) /CSM/VF/VFI5-C/VFI557Q.Seq.d/                       2302   0.0
VFF772 (VFF772Q) /CSM/VF/VFF7-C/VFF772Q.Seq.d/                       2286   0.0
VFE189 (VFE189Q) /CSM/VF/VFE1-D/VFE189Q.Seq.d/                       2286   0.0
VFC864 (VFC864Q) /CSM/VF/VFC8-C/VFC864Q.Seq.d/                       2286   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       2278   0.0
VFL880 (VFL880Q) /CSM/VF/VFL8-D/VFL880Q.Seq.d/                       2278   0.0
VFL628 (VFL628Q) /CSM/VF/VFL6-B/VFL628Q.Seq.d/                       2278   0.0
VFI577 (VFI577Q) /CSM/VF/VFI5-D/VFI577Q.Seq.d/                       2278   0.0
VFI526 (VFI526Q) /CSM/VF/VFI5-B/VFI526Q.Seq.d/                       2278   0.0
VFI515 (VFI515Q) /CSM/VF/VFI5-A/VFI515Q.Seq.d/                       2278   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432496) Dictyostelium discoideum cDNA clone:ddv18i23, 3' ...  1441   0.0    1
(BJ434356) Dictyostelium discoideum cDNA clone:ddv16k14, 3' ...  1435   0.0    1
(BJ434211) Dictyostelium discoideum cDNA clone:ddv16p01, 3' ...  1435   0.0    1
(BJ432365) Dictyostelium discoideum cDNA clone:ddv18a15, 3' ...  1435   0.0    1
(BJ428650) Dictyostelium discoideum cDNA clone:ddv12p17, 3' ...  1427   0.0    1
(BJ428459) Dictyostelium discoideum cDNA clone:ddv12c05, 3' ...  1427   0.0    1
(BJ431060) Dictyostelium discoideum cDNA clone:ddv9a23, 3' e...  1425   0.0    1
(BJ430289) Dictyostelium discoideum cDNA clone:ddv6p16, 3' e...  1425   0.0    1
(BJ345132) Dictyostelium discoideum cDNA clone:dda21p16, 3' ...  1423   0.0    1
(BJ434522) Dictyostelium discoideum cDNA clone:ddv24j02, 3' ...  1400   0.0    1
dna update 2009. 1. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   452   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   448   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        452   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   448   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   447   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   452   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          444   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   439   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   438   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   435   0.0
protein update 2009. 7.10
PSORT 

 psg: 0.78  gvh: 0.43  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFI557 is nuc
5' end seq. ID VFI557F
5' end seq. 
>VFI557F.Seq
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGCAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGAT
TACATGATGAAAATCNNNNNNNNNN
Length of 5' end seq. 625
3' end seq. ID VFI557Z
3' end seq. 
>VFI557Z.Seq
NNNNNNNNNNCTTATATGCCTCTGGTCGTACCACCGGGTATTGTTATGGATTCAGGTGAT
GGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGT
TTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGT
TACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCC
TACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAA
AAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGC
CCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACC
ACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTC
GTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTA
ACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATAC
TCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATC
TCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTTTCTAAATT
ATTTAATAAATAATAAAAAAACAAATTG
Length of 3' end seq. 748
Connected seq. ID VFI557P
Connected seq. 
>VFI557P.Seq
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGCAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGAT
TACATGATGAAAATC----------CTTATATGCCTCTGGTCGTACCACCGGGTATTGTT
ATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCA
CATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATC
TTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATC
AAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCA
TCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAAT
GAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCT
GGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGAT
TTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGT
ATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCA
CCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTC
CAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGA
AAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
Length of connected seq. 1353
Full length Seq ID VFI557E
Full length Seq. 
>VFI557E.Seq
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGCAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGA
AGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGA
AATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAAT
GCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGT
CATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTT
AGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGT
TGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCC
AGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAAT
TAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGC
TTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCC
ATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
Length of full length seq. 1199