NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ124
Library VF
(Link to library)
Clone ID VFJ124
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16272-1
Original site URL
Representative seq. ID VFJ124P
(Link to Original site)
Representative DNA sequence 
>VFJ124 (VFJ124Q) /CSM/VF/VFJ1-A/VFJ124Q.Seq.d/
GTTAGTNCTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCA
CGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTC
ATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGC
TGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGA
AATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGG
TTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAG
TGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTT
CACCAAATACGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTCT
CCAAGCCATCAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACAA
ATTAAAATTAACTCAAAAGAAAGCACACGTCCTTGAAATCCAAGTCAATGGTGGTTCAAT
CGTCGAAAAAGXXXXXXXXXXCAAGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTT
GCTGTTGCCAACTTTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTA
ATCGATGTTATCGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGT
GTCCGTAAATTACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCA
TGGCATCCATCCCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCAT
CGTGTCGAAAGAAACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAA
CAAATCTCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGT
GGTTTCGCTCACTACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCT
GGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCT
GCCCTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTT
CATCAAACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAG
GCTTAAATTCAATAAA
sequence update 2001.11.22
Translated Amino Acid sequence 
LVLLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKA
GMTHVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLS
DNFRRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSVVRVIAHTQVHK
LKLTQKKAHVLEIQVNGGSIVEK---

---QVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDVIGVTKGKGFNGVIKRWGVRKL
PRKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVERNKKIYRIGQAQPEDGKQIST
GKTEFDLTEKTINPMGGFAHYGMVKHEFLMLKGCVAGPRKRALTLRKSITTQTGRAALEK
ITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA*iq*
Translated Amino Acid sequence (All Frames) 
Frame A:
vsxskqq*qyess*i*sstsr*srfqtkkesrqtsr*sqvipkg*pypksplncihglqs
wydsrcqrfrktrfkdaqkrnr*srhhh*mstnvhcwfswlc*nrsrsqnlqnrlgstfk
**lp*kiiqklvqikikesfhqir*tirn*rr*kin*slspshqkemfsrpchcshsssq
ikinskestrp*npsqwwfnrrk---

---QVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDVIGVTKGKGFNGVIKRWGVRKL
PRKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVERNKKIYRIGQAQPEDGKQIST
GKTEFDLTEKTINPMGGFAHYGMVKHEFLMLKGCVAGPRKRALTLRKSITTQTGRAALEK
ITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA*iq*

Frame B:
LVLLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKA
GMTHVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLS
DNFRRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSVVRVIAHTQVHK
LKLTQKKAHVLEIQVNGGSIVEK---

---ksmvvqsskkltlllptlkrpstslvslpkmn*smlsv*pkvkvsmvslkdgvsvny
qerpikv*erlpvlvhgihpvlvppsqelvnsvtiivsketrrftesvklnqktvnksqp
vrlnsi*lkrlsiqwvvslttvwlnmns*clkvvllvqerep*p*ervsplklvvlpsrk
slsnssippaslvtvfikplkirlnisasrnqdqlrlkfnk

Frame C:
*xf*ttiti*vivnlklhvtvisvsdqereppdikvksshsqrmtvpkkst*lhswvtkl
v*ltlsei*knqvqrctkkkslkpspslnvhqctllv*lvmlkplkvskptkpsglni*v
itsvedytktgtnqnqrklspntlnntklkkvknqlkplskpskrdvqssvslltlkftn
*n*lkrkhtslksksmvvqsskk---

---sqwwfnrrks*lcccql*kdrqrhwclcrk*inrcyrcnqr*rfqwch*kmgcp*it
kkdp*rfkkgclywcmasipc*yhrpkswstrlpsscrkkqedlqnrssstrrr*tnlnr
*d*irfn*kdyqsngwfrslryg*t*ilna*rlccwskkeslnlkkeyhhsnwsccpren
hsqihrylqqvwsrsssnr*r*d*ifrrqeikin*glnsi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ124 (VFJ124Q) /CSM/VF/VFJ1-A/VFJ124Q.Seq.d/                       2464   0.0
VFN470 (VFN470Q) /CSM/VF/VFN4-C/VFN470Q.Seq.d/                       1298   0.0
VFN445 (VFN445Q) /CSM/VF/VFN4-B/VFN445Q.Seq.d/                       1298   0.0
VFM481 (VFM481Q) /CSM/VF/VFM4-D/VFM481Q.Seq.d/                       1298   0.0
VFM390 (VFM390Q) /CSM/VF/VFM3-D/VFM390Q.Seq.d/                       1298   0.0
VFM291 (VFM291Q) /CSM/VF/VFM2-D/VFM291Q.Seq.d/                       1298   0.0
VFL721 (VFL721Q) /CSM/VF/VFL7-A/VFL721Q.Seq.d/                       1298   0.0
VFL717 (VFL717Q) /CSM/VF/VFL7-A/VFL717Q.Seq.d/                       1298   0.0
VFL601 (VFL601Q) /CSM/VF/VFL6-A/VFL601Q.Seq.d/                       1298   0.0
VFL402 (VFL402Q) /CSM/VF/VFL4-A/VFL402Q.Seq.d/                       1298   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU052238) Dictyostelium discoideum slug cDNA, clone SLA404.     1298   0.0    1
(AU052147) Dictyostelium discoideum slug cDNA, clone SLA118.     1298   0.0    1
(AU040016) Dictyostelium discoideum slug cDNA, clone SLH448.     1298   0.0    1
(AU040010) Dictyostelium discoideum slug cDNA, clone SLH434.     1298   0.0    1
(AU039961) Dictyostelium discoideum slug cDNA, clone SLG843.     1298   0.0    1
(AU039914) Dictyostelium discoideum slug cDNA, clone SLG735.     1298   0.0    1
(AU039555) Dictyostelium discoideum slug cDNA, clone SLE743.     1298   0.0    1
(AU039545) Dictyostelium discoideum slug cDNA, clone SLE726.     1298   0.0    1
(AU039476) Dictyostelium discoideum slug cDNA, clone SLH858.     1298   0.0    1
(AU039341) Dictyostelium discoideum slug cDNA, clone SLH591.     1298   0.0    1
dna update 2009. 1.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34113) RecName: Full=60S ribosomal protein L3;  &JC4254(JC4254...   434   e-120
AM471157_1(AM471157|pid:none) Vitis vinifera contig VV78X211407....   286   8e-76
CR926481_1(CR926481|pid:none) Pongo abelii mRNA; cDNA DKFZp468G0...   285   2e-75
BC008492_1(BC008492|pid:none) Homo sapiens ribosomal protein L3,...   285   2e-75
(P39023) RecName: Full=60S ribosomal protein L3; AltName: Full=H...   285   2e-75
EF144245_1(EF144245|pid:none) Populus trichocarpa clone PX0011_L...   285   2e-75
EF146052_1(EF146052|pid:none) Populus trichocarpa clone WS0115_D...   285   3e-75
AK151142_1(AK151142|pid:none) Mus musculus bone marrow macrophag...   283   7e-75
EF145479_1(EF145479|pid:none) Populus trichocarpa clone WS01125_...   283   7e-75
AK151084_1(AK151084|pid:none) Mus musculus bone marrow macrophag...   283   9e-75
protein update 2009. 6.21
PSORT 

 psg: 0.67  gvh: 0.20  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.07
 nuc: 0.27  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFJ124 is cyt
5' end seq. ID VFJ124F
5' end seq. 
>VFJ124F.Seq
GTTAGTNCTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCA
CGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTC
ATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGC
TGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGA
AATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGG
TTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAG
TGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTT
CACCAAATACGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTCT
CCAAGCCATCAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACAA
ATTAAAATTAACTCAAAAGAAAGCACACGTCCTTGAAATCCAAGTCAATGGTGGTTCAAT
CGTCGAAAAAGNNNNNNNNNN
Length of 5' end seq. 621
3' end seq. ID VFJ124Z
3' end seq. 
>VFJ124Z.Seq
NNNNNNNNNNCAAGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTTGCCAA
CTTTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGATGTTAT
CGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGTAAATT
ACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCATCCATC
CCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTCGAAAG
AAACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATCTCAAC
CGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTCGCTCA
CTACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCAAGAAA
GAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAA
AATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAAACCGC
TGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAAATTCA
ATAAA
Length of 3' end seq. 665
Connected seq. ID VFJ124P
Connected seq. 
>VFJ124P.Seq
GTTAGTNCTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCA
CGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTC
ATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGC
TGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGA
AATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGG
TTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAG
TGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTT
CACCAAATACGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTCT
CCAAGCCATCAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACAA
ATTAAAATTAACTCAAAAGAAAGCACACGTCCTTGAAATCCAAGTCAATGGTGGTTCAAT
CGTCGAAAAAG----------CAAGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTT
GCTGTTGCCAACTTTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTA
ATCGATGTTATCGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGT
GTCCGTAAATTACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCA
TGGCATCCATCCCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCAT
CGTGTCGAAAGAAACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAA
CAAATCTCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGT
GGTTTCGCTCACTACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCT
GGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCT
GCCCTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTT
CATCAAACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAG
GCTTAAATTCAATAAA
Length of connected seq. 1266
Full length Seq ID -
Full length Seq. -
Length of full length seq. -