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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI211
Library VS
(Link to library)
Clone ID VSI211
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11939-1
Original site URL
Representative seq. ID VSI211E
(Link to Original site)
Representative DNA sequence 
>VSI211 (VSI211Q) /CSM/VS/VSI2-A/VSI211Q.Seq.d/
AATTCATTATCACTCTTGTAGGTGGTTTCGTATTACTCGAAAGTATTTTCATGATCATTT
GGACTGCAGTCGATAGACCAATTCCATTGGCTGAACCAATCTTTAAAGCTGGTGAAGCTC
AACTTCAATGTACTTCAGATTCTGAAGCTTGGTGGTATGTTTTCGTTTTCTATAAAGTAT
TTTACATTCTTTTTGGTGTATTTTTAGCTTTTAAAACAAGAAATGTTGTTGATTCTTTAA
ATGAATCAAAACCAATTACTTTAGCTTTATATAATTTAACATTTGTTATGGTTGTGTGCA
ATTGCACTTGGTTTTATTTTAAGAGATAATCCAATTGCAATTATTGTAATTCAAACAATT
GCAATTTTACTTGGATTTACAGTTACAGTATCAGTACTTTTCTTACCAAAGGTTTGGATG
ATTTTATCTGGTCAACAACATTCAATGGATTCAATTGGTACCTCAATGGATTCAATGGGA
AGATCAAATGGTAATACCACAGAAGCAGAGTCAACTAGAGGTTACACAAATAAAGATTAC
AATAATCAATCAGTTGGTAGATCATTCTCTGCTCATGCCACTGGTTTCAAAGATGTCGCT
CCATCAAATCATCCACAACTTGGTATTGTTTATACTGGTGGTGATAATTTCCCAAGAGTT
GGTTCTTCACAAACAGCTGCTTCAAGAAGTGAAGCTGAAATTAATGTTAGTAAAGATGTT
TTAGCAAAAGCAAATAAAAAGAATGAAGAAGA
sequence update 2001. 3.25
Translated Amino Acid sequence 
nslsll*vvsyyskvfs*sfglqsidqfhwlnqslklvklnfnvlqilklggmfsfsiky
ftfflvyf*llkqemllil*mnqnqll*lyii*HLLWLCAIALGFILRDNPIAIIVIQTI
AILLGFTVTVSVLFLPKVWMILSGQQHSMDSIGTSMDSMGRSNGNTTEAESTRGYTNKDY
NNQSVGRSFSAHATGFKDVAPSNHPQLGIVYTGGDNFPRVGSSQTAASRSEAEINVSKDV
LAKANKKNEE
Translated Amino Acid sequence (All Frames) 
Frame A:
nslsll*vvsyyskvfs*sfglqsidqfhwlnqslklvklnfnvlqilklggmfsfsiky
ftfflvyf*llkqemllil*mnqnqll*lyii*HLLWLCAIALGFILRDNPIAIIVIQTI
AILLGFTVTVSVLFLPKVWMILSGQQHSMDSIGTSMDSMGRSNGNTTEAESTRGYTNKDY
NNQSVGRSFSAHATGFKDVAPSNHPQLGIVYTGGDNFPRVGSSQTAASRSEAEINVSKDV
LAKANKKNEE


Frame B:
ihyhscrwfritrkyfhdhldcsr*tnsig*tnl*sw*sstsmyfrf*slvvcfrfl*si
lhsfwcifsf*nkkcc*ffk*iktnyfsfi*fnicygcvqlhlvlf*eiiqlqll*fkql
qfyldlqlqyqyfsyqrfg*fylvnniqwiqlvpqwiqwedqmvipqkqsqlevtqikit
iinqlvdhsllmplvskmslhqiihnlvlfilvviisqelvlhkqllqevklklmlvkmf
*qkqikrmkk


Frame C:
fiitlvggfvllesifmiiwtavdrpiplaepifkageaqlqctsdseawwyvfvfykvf
yilfgvflafktrnvvdslneskpitlalynltfvmvvcnctwfyfkr*sncnycnsnnc
nftwiysysistfltkglddfiwsttfngfnwylngfngkikw*yhrsrvn*rlhk*rlq
*sisw*iilcschwfqrcrsikssttwyclyww**fpkswfftnscfkk*s*n*c**rcf
sksk*ke*rr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI211 (VSI211Q) /CSM/VS/VSI2-A/VSI211Q.Seq.d/                       1491   0.0
VHD520 (VHD520Q) /CSM/VH/VHD5-A/VHD520Q.Seq.d/                       1415   0.0
VHK392 (VHK392Q) /CSM/VH/VHK3-D/VHK392Q.Seq.d/                       1411   0.0
CHR346 (CHR346Q) /CSM/CH/CHR3-B/CHR346Q.Seq.d/                       1390   0.0
VHB603 (VHB603Q) /CSM/VH/VHB6-A/VHB603Q.Seq.d/                       1370   0.0
VHP466 (VHP466Q) /CSM/VH/VHP4-C/VHP466Q.Seq.d/                       1344   0.0
VHL723 (VHL723Q) /CSM/VH/VHL7-A/VHL723Q.Seq.d/                       1298   0.0
VHN212 (VHN212Q) /CSM/VH/VHN2-A/VHN212Q.Seq.d/                       1245   0.0
VHI869 (VHI869Q) /CSM/VH/VHI8-C/VHI869Q.Seq.d/                       1219   0.0
VHO723 (VHO723Q) /CSM/VH/VHO7-A/VHO723Q.Seq.d/                       1160   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(DQ447637) Dictyostelium discoideum GluPR (GluPR) mRNA, comp...   904   0.0    3
(BJ443081) Dictyostelium discoideum cDNA clone:ddv51h23, 3' ...   886   0.0    3
(BJ385867) Dictyostelium discoideum cDNA clone:ddc56l11, 3' ...   874   0.0    3
(BJ438653) Dictyostelium discoideum cDNA clone:ddv38g05, 3' ...   856   0.0    3
(BJ446066) Dictyostelium discoideum cDNA clone:ddv61d18, 3' ...   841   0.0    4
(BJ437367) Dictyostelium discoideum cDNA clone:ddv34e02, 3' ...   807   0.0    4
(BJ443808) Dictyostelium discoideum cDNA clone:ddv54n05, 3' ...   787   0.0    4
(BJ444659) Dictyostelium discoideum cDNA clone:ddv57g04, 3' ...   755   0.0    3
(BJ442031) Dictyostelium discoideum cDNA clone:ddv48j18, 3' ...   765   0.0    4
(AU268326) Dictyostelium discoideum vegetative cDNA clone:VS...   904   0.0    2
dna update 2009. 7. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54ET0) RecName: Full=Putative metabotropic glutamate receptor;...   275   e-126
AF099033_1(AF099033|pid:none) Homo sapiens gamma-aminobutyric ac...    52   8e-07
AF069755_1(AF069755|pid:none) Homo sapiens orphan G protein-coup...    52   8e-07
AJ011318_1(AJ011318|pid:none) Rattus norvegicus mRNA for GABA-B ...    52   8e-07
AX023742_1(AX023742|pid:none) Sequence 3 from Patent WO0015786.        52   8e-07
(O75899) RecName: Full=Gamma-aminobutyric acid type B receptor s...    52   8e-07
AF074482_1(AF074482|pid:none) Rattus norvegicus GABA-B receptor ...    52   8e-07
(O88871) RecName: Full=Gamma-aminobutyric acid type B receptor s...    52   8e-07
AF058795_1(AF058795|pid:none) Rattus norvegicus GABA-B receptor ...    52   8e-07
BC035071_1(BC035071|pid:none) Homo sapiens gamma-aminobutyric ac...    52   8e-07
protein update 2009. 3.24
PSORT 

 psg: 0.96  gvh: 0.55  alm: 0.20  top: 0.57  tms: 0.07  mit: 0.26  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VSI211 is cyt
5' end seq. ID VSI211F
5' end seq. 
>VSI211F.Seq
AATTCATTATCACTCTTGTAGGTGGTTTCGTATTACTCGAAAGTATTTTCATGATCATTT
GGACTGCAGTCGATAGACCAATTCCATTGGCTGAACCAATCTTTAAAGCTGGTGAAGCTC
AACTTCAATGTACTTCAGATTCTGAAGCTTGGTGGTATGTTTTCGTTTTCTATAAAGNAT
TTTACATTCTTTTTGGTGTATTTNTAGCTNTTAAAACAAGAAATGTTGTTGATTCTTTAA
ATGAATCAAAACCAATTACTTTAGCTTTATATAATTTAACATTTGTTATGGTTGTGTGCA
ATTGCACTTGGTTTTATTTTAAGAGATAATCCAATTGCAATTATTGNAATTCAAACAATT
GCAATTTTACTTGGATTTACAGTTACAGTATCAGTACTTTTCTTACCAAAGGTTTGGATG
ATTTTAT----------
Length of 5' end seq. 427
3' end seq. ID VSI211Z
3' end seq. 
>VSI211Z.Seq
----------ATAAAGTATTTTACATTNTTTTTGGTGTATTTTTAGCTTTTAAAACAAGA
AATGTTGTTGATTCTTTAAATGAATCAAAACCAATTACTTTAGCTTTATATAATTTAACA
TTTGTTATGGTTGTTGCAATTGCACTTGGTTTTATTTTAAGAGATAATCCAATTGCAATT
ATTGTAATTCAAACAATTGCAATTTTACTTGGATTTACAGTTACAGTATCAGTACTTTTC
TTACCAAAGGTTTGGATGATTTTATCTGGTCAACAACATTCAATGGATTCAATTGGTACC
TCAATGGATTCAATGGGAAGATCAAATGGTAATACCACAGAAGCAGAGTCAACTAGAGGT
TACACAAATAAAGATTACAATAATCAATCAGTTGGTAGATCATTCTCTGCTCATGCCACT
GGTTTCAAAGATGTCGCTCCATCAAATCATCCACAACTTGGTATTGTTTATACTGGTGGT
GATAATTTCCCAAGAGTTGGTTCTTCACAAACAGCTGCTTCAAGAAGTGAAGCTGAAATT
AATGTTAGTAAAGATGTTTTAGCAAAAGCAAATAAAAAGAATGAAGAAGA
Length of 3' end seq. 580
Connected seq. ID VSI211P
Connected seq. 
>VSI211P.Seq
AATTCATTATCACTCTTGTAGGTGGTTTCGTATTACTCGAAAGTATTTTCATGATCATTT
GGACTGCAGTCGATAGACCAATTCCATTGGCTGAACCAATCTTTAAAGCTGGTGAAGCTC
AACTTCAATGTACTTCAGATTCTGAAGCTTGGTGGTATGTTTTCGTTTTCTATAAAGNAT
TTTACATTCTTTTTGGTGTATTTNTAGCTNTTAAAACAAGAAATGTTGTTGATTCTTTAA
ATGAATCAAAACCAATTACTTTAGCTTTATATAATTTAACATTTGTTATGGTTGTGTGCA
ATTGCACTTGGTTTTATTTTAAGAGATAATCCAATTGCAATTATTGNAATTCAAACAATT
GCAATTTTACTTGGATTTACAGTTACAGTATCAGTACTTTTCTTACCAAAGGTTTGGATG
ATTTTAT----------ATAAAGTATTTTACATTNTTTTTGGTGTATTTTTAGCTTTTAA
AACAAGAAATGTTGTTGATTCTTTAAATGAATCAAAACCAATTACTTTAGCTTTATATAA
TTTAACATTTGTTATGGTTGTTGCAATTGCACTTGGTTTTATTTTAAGAGATAATCCAAT
TGCAATTATTGTAATTCAAACAATTGCAATTTTACTTGGATTTACAGTTACAGTATCAGT
ACTTTTCTTACCAAAGGTTTGGATGATTTTATCTGGTCAACAACATTCAATGGATTCAAT
TGGTACCTCAATGGATTCAATGGGAAGATCAAATGGTAATACCACAGAAGCAGAGTCAAC
TAGAGGTTACACAAATAAAGATTACAATAATCAATCAGTTGGTAGATCATTCTCTGCTCA
TGCCACTGGTTTCAAAGATGTCGCTCCATCAAATCATCCACAACTTGGTATTGTTTATAC
TGGTGGTGATAATTTCCCAAGAGTTGGTTCTTCACAAACAGCTGCTTCAAGAAGTGAAGC
TGAAATTAATGTTAGTAAAGATGTTTTAGCAAAAGCAAATAAAAAGAATGAAGAAGA
Length of connected seq. 1007
Full length Seq ID VSI211E
Full length Seq. 
>VSI211E.Seq
AATTCATTATCACTCTTGTAGGTGGTTTCGTATTACTCGAAAGTATTTTCATGATCATTT
GGACTGCAGTCGATAGACCAATTCCATTGGCTGAACCAATCTTTAAAGCTGGTGAAGCTC
AACTTCAATGTACTTCAGATTCTGAAGCTTGGTGGTATGTTTTCGTTTTCTATAAAGTAT
TTTACATTCTTTTTGGTGTATTTTTAGCTTTTAAAACAAGAAATGTTGTTGATTCTTTAA
ATGAATCAAAACCAATTACTTTAGCTTTATATAATTTAACATTTGTTATGGTTGTGTGCA
ATTGCACTTGGTTTTATTTTAAGAGATAATCCAATTGCAATTATTGTAATTCAAACAATT
GCAATTTTACTTGGATTTACAGTTACAGTATCAGTACTTTTCTTACCAAAGGTTTGGATG
ATTTTATCTGGTCAACAACATTCAATGGATTCAATTGGTACCTCAATGGATTCAATGGGA
AGATCAAATGGTAATACCACAGAAGCAGAGTCAACTAGAGGTTACACAAATAAAGATTAC
AATAATCAATCAGTTGGTAGATCATTCTCTGCTCATGCCACTGGTTTCAAAGATGTCGCT
CCATCAAATCATCCACAACTTGGTATTGTTTATACTGGTGGTGATAATTTCCCAAGAGTT
GGTTCTTCACAAACAGCTGCTTCAAGAAGTGAAGCTGAAATTAATGTTAGTAAAGATGTT
TTAGCAAAAGCAAATAAAAAGAATGAAGAAGA
Length of full length seq. 752