| SKIP000887 | |
| SKIP ID | SKIP000887 |
| Organism(En) | the naked mole-rat/ Heterocephalus glaber |
| Organism(Ja) | ハダカデバネズミ |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | fibroblasts |
| Cell Tissue(Ja) | 線維芽細胞 |
| Cell Origin | Normal |
| Cell Name 1(En) | NMR-iPSCs clone 27 |
| Cell Name 1(Ja) | NMR-iPSCs clone 27 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | 0-9 |
| Sex | Male |
| Race(En) | the naked mole-rat/ Heterocephalus glaber |
| Race(Ja) | ハダカデバネズミ |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | NMR-iPSCs derive from the naked mole-rat (NMR, Heterocephalus glaber).NMR-iPSCs do not exhibit teratoma-forming tumorigenicity due to the species-specific activation of tumour-suppressor alternative reading frame (ARF) and a disruption mutation of the oncogene ES cell-expressed Ras (ERAS). |
| Description(Ja) | がん化耐性ハダカデバネズミ由来iPS細胞株。 tumour-suppressor alternative reading frame (ARF)の活性化及び癌原遺伝子ERASの欠失により腫瘍を形成しない。 |
| Cell Morphology | mouse ES-like |
| Grade | Research Grade |
| Vector | Retrovirus |
| Transgene | Oct4, Sox2, Klf4 and c-Myc |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | mitomycin C-treated SNL-STO (MSTO) feeder feeder layers on gelatin-coated six-well plates |
| Medium | DMEM/F12 (Sigma Aldrich) supplemented with 20% knockout serum replacement (Life Technologies), 1% penicillin/streptomycin, 2 mM L-glutamine, 0.1 mM NEAA, 0.1 mM β-mercaptoethanol (2-ME, Sigma Aldrich) and 4 ng/ml fibroblast growth factor 2 (PeproTech). |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | RNA-seq |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | Immunocytochemistry |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | Teratomas forming assay |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | G-band analyses |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Kyoko Miura |
| Author Name(Ja) | 三浦恭子 |
| Author Organization(En) | Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Hokkaido |
| Author Organization(Ja) | 北海道大学 遺伝子病制御研究所 動物機能医科学研究室 |
| Author Contact Email | miura[at]igm[dot]hokudai[dot]ac[dot]jp |
| PI Organization(En) | - |
| PI Organization(Ja) | - |
| PI Name(En) | - |
| PI Name(Ja) | - |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Hokkaido |
| Provider Organization(Ja) | 北海道大学 遺伝子病制御研究所 動物機能医科学研究室 |
| Provider Email | miura[at]igm[dot]hokudai[dot]ac[dot]jp |
| Provider URL | http://www.igm.hokudai.ac.jp/debanezumi/ |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 27161380 |
| DOI | 10.1038/ncomms11471 |
| Title | Tumour resistance in induced pluripotent stem cells derived from naked mole-rats. |
| Authors | Shingo Miyawaki, Hideyuki Okano et al. |
| Journal | NATURE COMMUNICATIONS |
| Year | 2015 |
| Volume | 7 |
| Issue | 11471 |
| Pages | - |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/27161380 |
| Free input | - |
| Note | - |