Organism(En) the naked mole-rat/ Heterocephalus glaber
Organism(Ja) ハダカデバネズミ
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) fibroblasts
Cell Tissue(Ja) 線維芽細胞
Cell Origin Normal
Cell Name 1(En) NMR-iPSCs clone 27
Cell Name 1(Ja) NMR-iPSCs clone 27
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range 0-9
Sex Male
Race(En) the naked mole-rat/ Heterocephalus glaber
Race(Ja) ハダカデバネズミ
Genetic Diagnosis --
Not Detected No
Description(En) NMR-iPSCs derive from the naked mole-rat (NMR, Heterocephalus glaber).NMR-iPSCs do not exhibit teratoma-forming tumorigenicity due to the species-specific activation of tumour-suppressor alternative reading frame (ARF) and a disruption mutation of the oncogene ES cell-expressed Ras (ERAS).
Description(Ja) がん化耐性ハダカデバネズミ由来iPS細胞株。 tumour-suppressor alternative reading frame (ARF)の活性化及び癌原遺伝子ERASの欠失により腫瘍を形成しない。
Cell Morphology mouse ES-like
Grade Research Grade
Vector Retrovirus
Transgene Oct4, Sox2, Klf4 and c-Myc
Adhesiveness -
Feeder Yes
Feeder Cell mitomycin C-treated SNL-STO (MSTO) feeder feeder layers on gelatin-coated six-well plates
Medium DMEM/F12 (Sigma Aldrich) supplemented with 20% knockout serum replacement (Life Technologies), 1% penicillin/streptomycin, 2 mM L-glutamine, 0.1 mM NEAA, 0.1 mM β-mercaptoethanol (2-ME, Sigma Aldrich) and 4 ng/ml fibroblast growth factor 2 (PeproTech).
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay RNA-seq
in vitro Differentiation Yes
in vitro Differentiation Assay Immunocytochemistry
in vivo Differentiation Yes
in vivo Differentiation Assay Teratomas forming assay
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay G-band analyses
Remaining Vector Detection -
Remaining Vector Detection Assay -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Kyoko Miura
Author Name(Ja) 三浦恭子
Author Organization(En) Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Hokkaido
Author Organization(Ja) 北海道大学 遺伝子病制御研究所 動物機能医科学研究室
Author Contact Email miura[at]igm[dot]hokudai[dot]ac[dot]jp
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Information Only
Provider Organization(En) Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Hokkaido
Provider Organization(Ja) 北海道大学 遺伝子病制御研究所 動物機能医科学研究室
Provider Email miura[at]igm[dot]hokudai[dot]ac[dot]jp
Provider URL
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 27161380
DOI 10.1038/ncomms11471
Title Tumour resistance in induced pluripotent stem cells derived from naked mole-rats.
Authors Shingo Miyawaki, Hideyuki Okano et al.
Year 2015
Volume 7
Issue 11471
Pages -
Free input -
Note -