| SE53 > S01 | |
| ID | SE53_S01 |
| Title | Tomato |
| Organism - Scientific Name | Solanum lycopersicum |
| Organism - ID | NCBI taxonomy:4081 |
| Compound - ID | - |
| Compound - Source | - |
| Preparation | <Biosource species> The two transgenic lines overexpressing milaculin gene (moneymaker background) were also used. <Organ> Fruits <Organ specification> Green and red fruits. A three grade color scale (green, orange, and red) was employed to evaluate tomato color. <Growth condition> Seedlings of Solanum lycopersicum were potted in 1/2000 a Wagner pot containing compost soil (Kureha, Tokyo, Japan) for the soil experiment. Seeds were sown in 5 cm × 5 cm × 5 cm (height × length × width) rockwool cubes and grown in a hydroponics system (565 mg l-1 NO- 3 , 15.7 mg l-1 NH+4 , 202.2 mg l-1 PO- 3 , 218.4 mg l-1 K+, 19.9 mg l-1 Mg+2 , 95.0 mg l-1 Ca+2 and micronutrients) in an environmentally controlled growth room at 25 °C/20 °C (light/dark) and 600 ppm CO2 concentration with a light/dark cycle of 16 h/8 h for the hydroponic culture (HC) experiment. Seedlings were placed in a netted-greenhouse located at the Gene Research Center in University of Tsukuba <Sampling and sampling date> The fruits were harvested in spring (a pilot and HC experiments) and late summer (the soil experiment) in 2006, 2008, and 2009. <Metabolism quenching method> All samples were frozen within 30 s after sampling in liquid nitrogen. The frozen samples were lyophilized sample_preparation_details_id SS01 |
| Comment | - |
| Analytical Method IDs | SE53_S01_M01, SE53_S01_M02, SE53_S01_M03 |