| Preparation |
Seedlings of Arabidopsis thaliana (Col-0) were grown in pots containing soil at 20℃ with a 16 h daily photoperiod. Six weeks after germination, the 12th or 13th expanded rosette leaves (rosette leaf), the 1st and 2nd expanded cauline leaves (cauline leaf), the upper part of the inflorescence (inflorescence), and first internode tissues (stem) were collected from eight individual Arabidopsis plants at stage 6.3 (Boyes et al., 2001) and stored at 80℃ until use. For metabolic phenotyping of Ds transposon insertion lines (Kuromori et al., 2004, 2006), 60 lines of homozygous seeds were grown on the half-strength MS medium plates at 20℃ with a 16 h daily photoperiod. Two weeks after germination, whole tissues of 20 seedlings were collected, weighed, and stored at 80℃. |