| Preparation |
Seeds from tomatoes (Solanum lycopersicum, cv. Reiyo) were sown in pots (volume, 2 L) with rockwool (Nittobo, Tokyo, Japan) and grown in a hydroponics system with a nutrient solution containing N, P, and K at 122, 21, and 156.6 mg/L, respectively (Otsuka Chemical, Osaka, Japan), in a growth chamber at 25 ℃/20 ℃ (light/dark) and 900 ppm CO2 concentration with a light/dark cycle of 16 h/8 h at Chiba University, Matsudo, Japan. Photosynthetic photon flux (PPF) level in the growth chamber was adjusted to 450-500 pmol m-2 s-1 when we measured at the meristem of each tomato plant (light source: Ceramic metal halide lamps). Subirrigation was applied twice a day with the nutrient solution, and plant material was harvested three weekdays after flowering in December 2009. |