SE52 > SS01
ID SE52_SS01
Title Metabolic profiling
Description The collected sample tissues were weighed and stored at −80°C until analysis. The frozen tissues of independent plants were homogenized in five volumes of 80% aqueous methanol containing 0.1% acetic acid, 0.5 mg/l of lidocaine, and d-camphor sulfonic acid (Tokyo Kasei, Tokyo, Japan) using a mixer mill (MM 300, Retsch) with a zirconia bead for 6 min at 20 Hz. Next, the samples were centrifuged at 15,000 g for 10 min and filtered (Ultrafree-MC filter, 0.2 μm; Millipore, Bedford, MA, USA). The sample extracts were then applied to an HLB μElution plate (Waters, Milford, MA, USA) that had been equilibrated with 80% aqueous methanol containing 0.1% acetic acid.
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