SE54 > SS01
ID SE54_SS01
Title High-throughput amino acid and glucosinolate analysis
Description A total of 200 seeds of each independent mutant and accession were homogenized using a mixer mill MM 200 (Retsch) in 80 μL of extraction buffer [40% acetonitrile in H2O with 25 μM hydroxyphenyl–glucosinolate (GSL) and 50 μM norleucine as internal standards].The extracts were diluted with 500 lL of LC–MS grade H2O and centrifuged (1,000 g) for 5 min. The supernatants were filtered through a CAPTIVA 0.45μlm filter (Varian).
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