DK945213
Clone id YMU02A01NGRL0008_J12
Library
Length 340
Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0008_J12. 5' end sequence.
Accession
Tissue type young leaves
Developmental stage sporophyte
Contig ID
Sequence
GTGCTGGGGGAGTAGTAGCTGATGATCAAGCTCTCACAGGAGCTGTGGATACTGAAGGAG
GAAACATTGAGGTTGTATTGCATGAGCAAGTAAATGTATATGTAAGACAGACAAAAATTA
ATCTGGTTGAGGATGATGGTCATGTCAAGAAACACCTTCAAGTAGGGGATGATGTAGTTG
GCGAGGAAGGGGCACGAGAAGAGGATGTTGATGCAGAGGAGCAAGAGTTGGCTGAAGATG
GAGAAGAAAGCGAAGATAACGAGGAATCACAAGATGGCGACGAGGAAGTAGACAATGAAG
AGGAGGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id Q05FH8
Definition sp|Q05FH8|RPOB_CARRP DNA-directed RNA polymerase subunit beta OS=Carsonella ruddii (strain PV)
Align length 68
Score (bit) 30.8
E-value 2.2
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK945213|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0008_J12, 5'
(310 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q05FH8|RPOB_CARRP DNA-directed RNA polymerase subunit beta OS... 31 2.2
sp|Q74JN8|UVRC_LACJO UvrABC system protein C OS=Lactobacillus jo... 30 2.9
sp|Q043W2|UVRC_LACGA UvrABC system protein C OS=Lactobacillus ga... 30 2.9
sp|A5IK42|DNAK_THEP1 Chaperone protein dnaK OS=Thermotoga petrop... 30 3.8
sp|Q9WYK6|DNAK_THEMA Chaperone protein dnaK OS=Thermotoga mariti... 30 3.8
sp|Q9JIS7|CAC1F_MOUSE Voltage-dependent L-type calcium channel s... 29 6.4
sp|Q1D9W6|CYSD_MYXXD Sulfate adenylyltransferase subunit 2 OS=My... 29 8.4
sp|O60840|CAC1F_HUMAN Voltage-dependent L-type calcium channel s... 29 8.4

>sp|Q05FH8|RPOB_CARRP DNA-directed RNA polymerase subunit beta
OS=Carsonella ruddii (strain PV) GN=rpoB PE=3 SV=1
Length = 1267

Score = 30.8 bits (68), Expect = 2.2
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
Frame = -3

Query: 218 LCINILFSCPFLANYIIPYLKVFLDMTI---ILNQINFCLSYIYIYLLMQYNLNVSSFSI 48
L I I+F C + N + L + I I NQ+N C+ Y + + + N + + I
Sbjct: 1072 LFIKIIFKCIYDKNINLNIFNNSLVLKIFKNIKNQLNVCVHNFYNFNVNKVNNIIKTIGI 1131

Query: 47 HSSCESLI 24
+ +CE L+
Sbjct: 1132 NKNCELLL 1139


>sp|Q74JN8|UVRC_LACJO UvrABC system protein C OS=Lactobacillus
johnsonii GN=uvrC PE=3 SV=1
Length = 600

Score = 30.4 bits (67), Expect = 2.9
Identities = 19/50 (38%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Frame = +3

Query: 54 EGGNIEV-----VLHEQVNVYVRQTKINLVEDDGHVKKHLQVGDDVVGEE 188
+GG I+V VL ++NV + +V+DD H HL GD GEE
Sbjct: 460 DGGQIQVEACLDVLRNELNVNIPVA--GMVKDDHHRTNHLIFGDPTKGEE 507


>sp|Q043W2|UVRC_LACGA UvrABC system protein C OS=Lactobacillus
gasseri (strain ATCC 33323 / DSM 20243) GN=uvrC PE=3
SV=1
Length = 600

Score = 30.4 bits (67), Expect = 2.9
Identities = 19/50 (38%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Frame = +3

Query: 54 EGGNIEV-----VLHEQVNVYVRQTKINLVEDDGHVKKHLQVGDDVVGEE 188
+GG I+V VL ++NV + +V+DD H HL GD GEE
Sbjct: 460 DGGQIQVDACLDVLRNELNVNIPVA--GMVKDDHHRTNHLIFGDPTQGEE 507


>sp|A5IK42|DNAK_THEP1 Chaperone protein dnaK OS=Thermotoga
petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995)
GN=dnaK PE=2 SV=1
Length = 596

Score = 30.0 bits (66), Expect = 3.8
Identities = 14/51 (27%), Positives = 27/51 (52%)
Frame = +3

Query: 12 VVADDQALTGAVDTEGGNIEVVLHEQVNVYVRQTKINLVEDDGHVKKHLQV 164
V+ D LT ++ +GG E ++ + VR++KI +DG + ++V
Sbjct: 372 VLVDVTPLTLGIEVKGGLFEPIIPRNTKIPVRKSKIFTTVEDGQTEVEIRV 422


>sp|Q9WYK6|DNAK_THEMA Chaperone protein dnaK OS=Thermotoga maritima
GN=dnaK PE=2 SV=1
Length = 596

Score = 30.0 bits (66), Expect = 3.8
Identities = 14/51 (27%), Positives = 27/51 (52%)
Frame = +3

Query: 12 VVADDQALTGAVDTEGGNIEVVLHEQVNVYVRQTKINLVEDDGHVKKHLQV 164
V+ D LT ++ +GG E ++ + VR++KI +DG + ++V
Sbjct: 372 VLVDVTPLTLGIEVKGGLFEPIIPRNTKIPVRKSKIFTTVEDGQTEVEIRV 422


>sp|Q9JIS7|CAC1F_MOUSE Voltage-dependent L-type calcium channel
subunit alpha-1F OS=Mus musculus GN=Cacna1f PE=1 SV=1
Length = 1985

Score = 29.3 bits (64), Expect = 6.4
Identities = 15/49 (30%), Positives = 31/49 (63%), Gaps = 3/49 (6%)
Frame = -3

Query: 161 LKVFLDMTII---LNQINFCLSYIYIYLLMQYNLNVSSFSIHSSCESLI 24
+++ L MT+ L++ +FC S+ + L+ ++++ SF IHSS S++
Sbjct: 922 VEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVV 970


>sp|Q1D9W6|CYSD_MYXXD Sulfate adenylyltransferase subunit 2
OS=Myxococcus xanthus (strain DK 1622) GN=cysD PE=3 SV=1
Length = 305

Score = 28.9 bits (63), Expect = 8.4
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = +3

Query: 33 LTGAVDTEGGNIEVVLHEQVNVYVRQTKINLVEDD 137
L+GA+++ +E V+HE VN + + L++ D
Sbjct: 257 LSGAIESSATTVETVIHEMVNARQSERQGRLIDHD 291


>sp|O60840|CAC1F_HUMAN Voltage-dependent L-type calcium channel
subunit alpha-1F OS=Homo sapiens GN=CACNA1F PE=1 SV=1
Length = 1966

Score = 28.9 bits (63), Expect = 8.4
Identities = 15/49 (30%), Positives = 31/49 (63%), Gaps = 3/49 (6%)
Frame = -3

Query: 161 LKVFLDMTII---LNQINFCLSYIYIYLLMQYNLNVSSFSIHSSCESLI 24
+++ L MT+ L++ +FC S+ + L+ ++++ SF IHSS S++
Sbjct: 906 VEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVV 954


tr_hit_id Q9G8P4
Definition tr|Q9G8P4|Q9G8P4_NAEGR ABC transporter subunit OS=Naegleria gruberi
Align length 65
Score (bit) 35.4
E-value 1.4
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK945213|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0008_J12, 5'
(310 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|Q9G8P4|Q9G8P4_NAEGR ABC transporter subunit OS=Naegleria grub... 35 1.4
tr|B8F261|B8F261_BORGA Putative uncharacterized protein OS=Borre... 33 5.5
tr|A0C7R0|A0C7R0_PARTE Chromosome undetermined scaffold_156, who... 33 7.2
tr|Q0QB69|Q0QB69_9MUSC NADH-ubiquinone oxidoreductase chain 2 (F... 33 9.4
tr|Q0QB27|Q0QB27_9MUSC NADH-ubiquinone oxidoreductase chain 2 (F... 33 9.4

>tr|Q9G8P4|Q9G8P4_NAEGR ABC transporter subunit OS=Naegleria gruberi
GN=yejU PE=4 SV=1
Length = 210

Score = 35.4 bits (80), Expect = 1.4
Identities = 24/65 (36%), Positives = 38/65 (58%), Gaps = 1/65 (1%)
Frame = -3

Query: 212 INILFSCPFLANYIIPYLKV-FLDMTIILNQINFCLSYIYIYLLMQYNLNVSSFSIHSSC 36
I++ + F+ NYI+ YL + F+ ++ IL I F + YIY + N+N+SS I SS
Sbjct: 25 ISLDYYSKFIYNYIL-YLHISFIIISYILFFIIFIIYVSYIYSINIININISSIGIFSSF 83

Query: 35 ESLII 21
+L I
Sbjct: 84 YTLFI 88


>tr|B8F261|B8F261_BORGA Putative uncharacterized protein OS=Borrelia
garinii Far04 GN=BGAFAR04_C0003 PE=4 SV=1
Length = 242

Score = 33.5 bits (75), Expect = 5.5
Identities = 20/65 (30%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = -3

Query: 206 ILFSCPFLANYIIPYLKVFLD-MTIILNQINFCLSYIYIYLLMQYNLNVSSFSIHSSCES 30
+ FSC NY I Y+ F+ + ++ IN+ +SYI Y + N +S + S +
Sbjct: 2 LFFSCTLPFNYFISYINYFISYINYFISYINYFISYIN-YFISYINYFISYINYFISYIN 60

Query: 29 LIISY 15
ISY
Sbjct: 61 YFISY 65


>tr|A0C7R0|A0C7R0_PARTE Chromosome undetermined scaffold_156, whole
genome shotgun sequence OS=Paramecium tetraurelia
GN=GSPATT00035958001 PE=4 SV=1
Length = 220

Score = 33.1 bits (74), Expect = 7.2
Identities = 24/78 (30%), Positives = 39/78 (50%), Gaps = 7/78 (8%)
Frame = -3

Query: 227 LLLLCINILFSCPFLANYI-------IPYLKVFLDMTIILNQINFCLSYIYIYLLMQYNL 69
+L CINILF C + A+ I L VF +++I+ + LSY Y Q+N+
Sbjct: 138 ILCFCINILFQCIYYASGITGAMRKSYKTLNVFGNLSILGMFLQIFLSYTY-----QFNI 192

Query: 68 NVSSFSIHSSCESLIISY 15
V +F + S + +S+
Sbjct: 193 LVFAFRVLSCIYAKFLSH 210


>tr|Q0QB69|Q0QB69_9MUSC NADH-ubiquinone oxidoreductase chain 2
(Fragment) OS=Drosophila cf. cheda BCW-2006 GN=ND2 PE=3
SV=1
Length = 341

Score = 32.7 bits (73), Expect = 9.4
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = -3

Query: 188 FLANYIIPYLKVFLDMTIILNQINFCLSYIYIYLLMQYNLNVSSFSIHSSCESLIISYYS 9
FL+ +P FL I++ Q+ FC SY + +L+ L F + + +++YY
Sbjct: 244 FLSLGGLPPFLGFLPKWIVIQQLTFCSSYFLLMILLMTTLITLFFYLRICFSAFMLNYYE 303

Query: 8 PS 3
S
Sbjct: 304 NS 305


>tr|Q0QB27|Q0QB27_9MUSC NADH-ubiquinone oxidoreductase chain 2
(Fragment) OS=Drosophila bipatita GN=ND2 PE=3 SV=1
Length = 341

Score = 32.7 bits (73), Expect = 9.4
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = -3

Query: 188 FLANYIIPYLKVFLDMTIILNQINFCLSYIYIYLLMQYNLNVSSFSIHSSCESLIISYYS 9
FL+ +P FL I++ Q+ FC SY + +L+ L F + + +++YY
Sbjct: 244 FLSLGGLPPFLGFLPKWIVIQQLTFCSSYFLLMILLMTTLITLFFYLRICFSAFMLNYYE 303

Query: 8 PS 3
S
Sbjct: 304 NS 305