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NBDC - LSDB Archive
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AcEST | BlastX Result : Swiss-Prot | BlastX Result : TrEMBL | AcESTcontig | About Libraries
DK945321
Clone id YMU02A01NGRL0008_O24
Library
Length 537
Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0008_O24. 5' end sequence.
Accession
Tissue type young leaves
Developmental stage sporophyte
Contig ID
Sequence 
GTCCTTTCTGGGCCAGGTCAGACTCTGCATCGCGAGGATGAAGGCGGGCGCCGTTCTACA
AAGCAAGGCGCCATGAATGCTCGGGGCTTGTTGCGTAGTTCAGGACGAGCCCTGTAGCCC
TACTGTGCAAGGCATCGAATCATCGATTCGTTTTTTTGCTGTGCATCCCGAGGATGAAGG
CGGCCTTGATTAACGATGATGCAGAAGGGCTGCGATGTCTGCTTGCTGGGGATGCTCTGT
GCGCATTGAATGCCTCATGGGAGAGTCCTTTTCAACGCGTACAGCTTTTTGCGCCTACTG
TGTTGCTCACAACCTACTGAGAACTTGCCAATTGGTGCTGCGAACAGTAGTGTGTGCTTG
TATAAGAGGAGGTGGTCGCCTCTTTCTCTCTCCATCTAAGTCTGCCTCTTTGTCTCCATT
TGAATGCGTGAGAGCATCTCGAGTGTCACTGTTGATGGCTTGCAACCGGGACAATTGTTG
TTCGTGTGCTGGCAAAGCGGATGAATTGTGCATCAGCAATGGTGATTCTCTCGGAGC
■■Homology search results ■■ -
sp_hit_id P58069
Definition sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus musculus
Align length 54
Score (bit) 32.0
E-value 1.9
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK945321|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0008_O24, 5'
         (537 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus mus...    32   1.9  
sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus no...    32   2.5  
sp|A3LX75|VPS27_PICST Vacuolar protein sorting-associated protei...    30   7.4  
sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase OS...    30   9.7  
sp|Q53515|NODU_RHITR Nodulation protein U OS=Rhizobium tropici G...    30   9.7  
sp|P31957|NODU_RHISN Nodulation protein U OS=Rhizobium sp. (stra...    30   9.7  
sp|Q4WI96|KU80_ASPFU Protein Ku80 OS=Aspergillus fumigatus GN=ku...    30   9.7  
sp|Q7VRS2|GUAA_BLOFL GMP synthase [glutamine-hydrolyzing] OS=Blo...    30   9.7  
sp|Q8GWK2|AP2L4_ARATH AP2-like ethylene-responsive transcription...    30   9.8  

>sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus
           musculus GN=Rasa2 PE=1 SV=1
          Length = 848

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 9/54 (16%)
 Frame = -2

Query: 533 RENHHC*CTIHP---------LCQHTNNNCPGCKPSTVTLEMLSRIQMETKRQT 399
           R NH+   + HP          CQ T+ + PGCKP T  +    +I ++  R+T
Sbjct: 705 RCNHNRLSSFHPSAYLNGNWLCCQETSESTPGCKPCTAGIPADIQIDIDEDRET 758


>sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus
           norvegicus GN=Rasa2 PE=2 SV=1
          Length = 847

 Score = 31.6 bits (70), Expect = 2.5
 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 9/54 (16%)
 Frame = -2

Query: 533 RENHHC*CTIHP---------LCQHTNNNCPGCKPSTVTLEMLSRIQMETKRQT 399
           R NH+   + HP          CQ T+   PGCKP T  +    +I ++  R+T
Sbjct: 704 RCNHNRLSSFHPSAYLNGNWLCCQETSEGTPGCKPCTAGIPADIQIDIDEDRET 757


>sp|A3LX75|VPS27_PICST Vacuolar protein sorting-associated protein
           27 OS=Pichia stipitis GN=VPS27 PE=3 SV=2
          Length = 732

 Score = 30.0 bits (66), Expect = 7.4
 Identities = 15/41 (36%), Positives = 22/41 (53%)
 Frame = +2

Query: 398 KSASLSPFECVRASRVSLLMACNRDNCCSCAGKADELCISN 520
           +S SL P +C+R+ +  L M  +  N  S   K  +LCI N
Sbjct: 44  RSKSLPPIQCMRSLKKRLGMTYSNPNLLSSTLKLVDLCIKN 84


>sp|Q6AJY9|PNP_DESPS Polyribonucleotide nucleotidyltransferase
           OS=Desulfotalea psychrophila GN=pnp PE=3 SV=1
          Length = 692

 Score = 29.6 bits (65), Expect = 9.7
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = +2

Query: 371 GGRLFLSPSKSASLSPFECVRASRVSLLMACNRDNCCSCAGKADEL 508
           GGR+     K      F  + +S + L++AC R+      GKADEL
Sbjct: 150 GGRIAYIDGKYVLNPTFAELESSSMDLMVACTRNAIVMVEGKADEL 195


>sp|Q53515|NODU_RHITR Nodulation protein U OS=Rhizobium tropici
           GN=nodU PE=3 SV=1
          Length = 575

 Score = 29.6 bits (65), Expect = 9.7
 Identities = 19/55 (34%), Positives = 24/55 (43%), Gaps = 2/55 (3%)
 Frame = +3

Query: 132 ASNHRFVFLLCIPRMKAALINDDAEGLR--CLLAGDALCALNASWESPFQRVQLF 290
           AS H F+ LL +  M  A+      G R  C+  G   C LN  W S  +   LF
Sbjct: 275 ASFHSFLELLLVREMALAMQRHSLPGPRNLCIAGG---CGLNIKWNSALRETGLF 326


>sp|P31957|NODU_RHISN Nodulation protein U OS=Rhizobium sp. (strain
           NGR234) GN=nodU PE=3 SV=2
          Length = 558

 Score = 29.6 bits (65), Expect = 9.7
 Identities = 19/55 (34%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
 Frame = +3

Query: 132 ASNHRFVFLLCIPRMKAALINDDAEGLR--CLLAGDALCALNASWESPFQRVQLF 290
           AS+H F+  L +  M  AL +    G R  C+  G   C LN  W S  +   LF
Sbjct: 275 ASSHFFLERLLVDEMANALQHHPLPGARNLCIAGG---CGLNIKWNSALRETGLF 326


>sp|Q4WI96|KU80_ASPFU Protein Ku80 OS=Aspergillus fumigatus GN=ku80
           PE=3 SV=1
          Length = 725

 Score = 29.6 bits (65), Expect = 9.7
 Identities = 11/24 (45%), Positives = 18/24 (75%)
 Frame = +2

Query: 104 DEPCSPTVQGIESSIRFFAVHPED 175
           D+  SP +  I+S+IR+ A+HP+D
Sbjct: 495 DDSFSPVLHRIDSAIRYRAIHPDD 518


>sp|Q7VRS2|GUAA_BLOFL GMP synthase [glutamine-hydrolyzing]
           OS=Blochmannia floridanus GN=guaA PE=3 SV=1
          Length = 526

 Score = 29.6 bits (65), Expect = 9.7
 Identities = 14/26 (53%), Positives = 17/26 (65%)
 Frame = +3

Query: 456 WLATGTIVVRVLAKRMNCASAMVILS 533
           WLA GTI   V+   M+CASA +I S
Sbjct: 333 WLAQGTIYSDVIESGMSCASANIIKS 358


>sp|Q8GWK2|AP2L4_ARATH AP2-like ethylene-responsive transcription
           factor At2g41710 OS=Arabidopsis thaliana GN=At2g41710
           PE=2 SV=2
          Length = 428

 Score = 29.6 bits (65), Expect = 9.8
 Identities = 12/21 (57%), Positives = 14/21 (66%)
 Frame = -1

Query: 168 GCTAKKRIDDSMPCTVGLQGS 106
           GCTAK+RI    PCT G + S
Sbjct: 49  GCTAKERISKMPPCTAGKRSS 69
tr_hit_id Q5M9W6
Definition tr|Q5M9W6|Q5M9W6_TOBAC Putative uncharacterized protein orf114c OS=Nicotiana tabacum
Align length 50
Score (bit) 35.0
E-value 2.5
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK945321|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0008_O24, 5'
         (537 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q5M9W6|Q5M9W6_TOBAC Putative uncharacterized protein orf114c ...    35   2.5  
tr|A6QP32|A6QP32_BOVIN KRT85 protein OS=Bos taurus GN=KRT85 PE=2...    35   2.5  
tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by t...    35   2.5  
tr|A9V705|A9V705_MONBE Predicted protein OS=Monosiga brevicollis...    34   4.2  
tr|B6KGI6|B6KGI6_TOXGO Putative uncharacterized protein OS=Toxop...    34   4.3  
tr|Q5L175|Q5L175_GEOKA Methyl-accepting chemotaxis protein OS=Ge...    34   5.6  
tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein OS=Leish...    33   7.3  
tr|B6QUH3|B6QUH3_PENMA BZIP transcription factor, putative OS=Pe...    33   7.3  
tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome OS=Aspe...    33   7.3  
tr|A0BR77|A0BR77_PARTE Chromosome undetermined scaffold_122, who...    33   9.3  
tr|B7YFF6|B7YFF6_9BACI Methyl-accepting chemotaxis sensory trans...    33   9.5  
tr|B3KVU5|B3KVU5_HUMAN cDNA FLJ41463 fis, clone BRSTN2017237 OS=...    33   9.5  

>tr|Q5M9W6|Q5M9W6_TOBAC Putative uncharacterized protein orf114c
           OS=Nicotiana tabacum GN=orf114c PE=4 SV=1
          Length = 114

 Score = 35.0 bits (79), Expect = 2.5
 Identities = 17/50 (34%), Positives = 25/50 (50%)
 Frame = +2

Query: 140 SSIRFFAVHPEDEGGLDXXXXXXAAMSACWGCSVRIECLMGESFSTRTAF 289
           S IR F + P     +       +  S+ WG S  +ECL+GES S+ T +
Sbjct: 55  SKIRSFKLKPTSISSVGRQGSSLSIHSSYWGISALVECLVGESVSSATLY 104


>tr|A6QP32|A6QP32_BOVIN KRT85 protein OS=Bos taurus GN=KRT85 PE=2
           SV=1
          Length = 493

 Score = 35.0 bits (79), Expect = 2.5
 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
 Frame = +2

Query: 8   LGQVRLCIARMKAGAV---LQSKAP*MLGACCVVQDEPCSPTVQGIESSIR 151
           +G + +C++R + G +   L S  P  LG   +     CSP+V G  SS+R
Sbjct: 438 VGSINICVSRSQGGVICGDLDSTVPRGLGGTAISSSALCSPSVGGFCSSVR 488


>tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by
           transcript evidence OS=Caenorhabditis elegans GN=M02G9.2
           PE=2 SV=3
          Length = 398

 Score = 35.0 bits (79), Expect = 2.5
 Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 6/65 (9%)
 Frame = +2

Query: 353 CACIRGGGR-LFLSPSKSASLSPFECVRASRVSLLMACNRDN----CCSCAGKADEL-CI 514
           C C  G  R +   PS  A   P+ CV     S L +CNR N    C S   +A E  C 
Sbjct: 65  CVCTEGNVRNIITGPSLPALFKPYNCVSTCESSCLESCNRLNFNMKCSSVCSEACEFTCA 124

Query: 515 SNGDS 529
             G S
Sbjct: 125 KQGTS 129


>tr|A9V705|A9V705_MONBE Predicted protein OS=Monosiga brevicollis
            GN=10710 PE=3 SV=1
          Length = 3468

 Score = 34.3 bits (77), Expect = 4.2
 Identities = 33/145 (22%), Positives = 53/145 (36%), Gaps = 2/145 (1%)
 Frame = +2

Query: 89   CCVVQDEPCSPTVQGIESSIRFFAVHPEDEGGLDXXXXXXAAMSACWGCSVRIECLMGES 268
            C   + EP  P         +  + +  D+G             AC    +R E L+G  
Sbjct: 2854 CRAEETEPVEPNPNSTACFAQNSSTYQRDDG----VCAPCTTADAC----LRNETLIGSC 2905

Query: 269  FSTRTAFCAYCVAHNLLRTCQLVLRTVVCACIRGGGRLFLSPSKSASLSPFECVRASRVS 448
                +  C  C  H   RTC  +  T   AC  G   L  +P +   ++     +    +
Sbjct: 2906 SKDTSPICFPC--HPSCRTCTGISSTDCVACATG---LVFAPDQGTCVTTCAMGQYPNAT 2960

Query: 449  LL--MACNRDNCCSCAGKADELCIS 517
            L   + C+ D C SC+G   + C+S
Sbjct: 2961 LASCLPCHED-CVSCSGAGPDQCLS 2984


>tr|B6KGI6|B6KGI6_TOXGO Putative uncharacterized protein
           OS=Toxoplasma gondii ME49 GN=TGME49_039470 PE=4 SV=1
          Length = 1984

 Score = 34.3 bits (77), Expect = 4.3
 Identities = 30/95 (31%), Positives = 45/95 (47%), Gaps = 22/95 (23%)
 Frame = -1

Query: 483 EQQLSRLQAINSDTRDA----------------LTHSNGDKEADLDGERKRRPPPLIQAH 352
           E++LSR QA   DTR+A                L+HS G     + GE  R P  L+ + 
Sbjct: 299 EERLSRAQAKERDTREAVKKDIETFEMNLQRLGLSHS-GSAGVSVSGEYLRSPGSLLASL 357

Query: 351 TTV------RSTNWQVLSRL*ATQ*AQKAVRVEKD 265
           + +      R  N ++L+RL A + AQ+  R E+D
Sbjct: 358 SKLASPSFGRQENMKMLARLHAARRAQEVSRKERD 392


>tr|Q5L175|Q5L175_GEOKA Methyl-accepting chemotaxis protein
           OS=Geobacillus kaustophilus GN=GK1020 PE=4 SV=1
          Length = 658

 Score = 33.9 bits (76), Expect = 5.6
 Identities = 19/67 (28%), Positives = 29/67 (43%)
 Frame = -1

Query: 522 PLLMHNSSALPAHEQQLSRLQAINSDTRDALTHSNGDKEADLDGERKRRPPPLIQAHTTV 343
           P+L         H Q   +  A N+  R  L H  G +E +LDGE++      +   TT+
Sbjct: 200 PVLFDAKGTAVVHPQYKGKNMAKNATVRYMLEHKKGMREYELDGEQR------VMYFTTI 253

Query: 342 RSTNWQV 322
               W+V
Sbjct: 254 PELGWKV 260


>tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein
           OS=Leishmania braziliensis GN=LbrM20_V2.4220 PE=4 SV=1
          Length = 1189

 Score = 33.5 bits (75), Expect = 7.3
 Identities = 18/54 (33%), Positives = 31/54 (57%)
 Frame = -1

Query: 534 PRESPLLMHNSSALPAHEQQLSRLQAINSDTRDALTHSNGDKEADLDGERKRRP 373
           P   PL+   SSA P  E  ++   A+++++  A T + G+ ++   GE+KRRP
Sbjct: 431 PTLPPLVKAESSAAPTAESPVANRGALSAESA-AATANTGESDSSPAGEKKRRP 483


>tr|B6QUH3|B6QUH3_PENMA BZIP transcription factor, putative
           OS=Penicillium marneffei ATCC 18224 GN=PMAA_009160 PE=4
           SV=1
          Length = 661

 Score = 33.5 bits (75), Expect = 7.3
 Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
 Frame = -1

Query: 513 MHNSSALPAHEQQLSRLQAINSDTRDAL-THSNGDKEADLDGERKRRPPPLIQAHTTVRS 337
           +HN+ ALP  +Q+  ++ A+N++  +AL T    D   D   +   +P  L QA   +R 
Sbjct: 564 LHNNLALPTKQQENEQVFALNAEQYNALTTFQEDDHNMDFGDKPSGQPSNLQQALAAMRG 623

Query: 336 TN 331
            N
Sbjct: 624 NN 625


>tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome
           OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513)
           GN=An16g07260 PE=3 SV=1
          Length = 636

 Score = 33.5 bits (75), Expect = 7.3
 Identities = 17/58 (29%), Positives = 38/58 (65%)
 Frame = -1

Query: 513 MHNSSALPAHEQQLSRLQAINSDTRDALTHSNGDKEADLDGERKRRPPPLIQAHTTVR 340
           +HN+ A+P+ +Q+  ++ A+N+D  ++LT  +   +A +D +++++P  L QA   +R
Sbjct: 528 LHNTLAMPSKQQEQEQVFALNADQYNSLTTFD---DAGVDYKQQQQPSNLQQALAAMR 582


>tr|A0BR77|A0BR77_PARTE Chromosome undetermined scaffold_122, whole
            genome shotgun sequence OS=Paramecium tetraurelia
            GN=GSPATT00031274001 PE=4 SV=1
          Length = 4719

 Score = 33.1 bits (74), Expect = 9.3
 Identities = 22/104 (21%), Positives = 41/104 (39%), Gaps = 7/104 (6%)
 Frame = +2

Query: 215  MSACWGCSVRIECLMGE-SFSTRTAFCAYCVAHNLLRTCQLVLRTVVCACIRGGGRLFLS 391
            ++ C  C    +C   + ++  +   C  CVA      C    + +VC       + +L 
Sbjct: 3600 INGCLSCLSNTKCTACDLTYYLKNQSCTTCVAG--CNVCTNDTQCIVCTT-----KWYLD 3652

Query: 392  PSKSASLSPFECVRASRVSLLMAC------NRDNCCSCAGKADE 505
             +K+ +  P EC   S  S+ + C         NC  C G +++
Sbjct: 3653 SNKTCTKCPSECATCSSGSVCLTCEANYKLQNSNCIKCTGYSNQ 3696