DK945564
Clone id YMU02A01NGRL0009_L15
Library
Length 665
Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0009_L15. 5' end sequence.
Accession
Tissue type young leaves
Developmental stage sporophyte
Contig ID
Sequence
TTCAGGACGAGCCCTGTAGCCCTACTGTGCAAAGCATCGAATCATCGATTCGTTTTTTGC
TGTGCATCCCGAGGATGAAGGCGGCCTTGATTAACGATGATGCAGAAGGGCTGCGATGTC
TGCTTGCTGGGGATGCTCTGTGCGCATTGAATGCCTCATGGGAGAGTCCTTTTCAACGCG
TACAGCTTTTTGCGCCTACTGTGTTGCTCACAGCCTACTGAGAACTTGCCAATTGGTGCT
GCGAACAGTAGTGTGTGCTTGTATAAGAGGAGGTGGTCGCCTCTTTCTCTCTCCATCTAA
GTCTGCCTCTTTGTCTCCATTTGAATGCGTGAGAGCATCTTGAGTGTCACTGTTGATGGC
TTGCAACCGGGACAATTGTTGTTCGTGTGCTGGCAAAGCGGAAGAATTGTGCATCAGCAA
TGGTGATTCTCTCGGAGCTGAGAGCATGGACTTCGAAGGGGGTGAGCTCCATTGAAGGTT
TTCTTTTATTTGATGCTTTGTAAACCTATTCTGGCGAAGTATTCGAGCATCTCTGTGTCG
AAATTGCGGATCTGATGTGCTTCATTCCTAGCAGGAAACGATATAATATCTGTATCGGTG
ATGTAATTTTTGGGTGCTATAATGTTGGTTATGAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAA
■■Homology search results ■■ -
sp_hit_id Q8CIN9
Definition sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus norvegicus
Align length 65
Score (bit) 31.6
E-value 3.5
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK945564|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0009_L15, 5'
(620 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Ratt... 32 3.5
sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=n... 31 4.5
sp|Q4R2Z8|ZN541_MACFA Zinc finger protein 541 (Fragment) OS=Maca... 27 5.4
sp|A4R6X5|RT106_MAGGR Histone chaperone RTT106 OS=Magnaporthe gr... 31 5.9
sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus mus... 31 5.9
sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus no... 30 7.7
sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage... 30 7.7

>sp|Q8CIN9|RFFL_RAT E3 ubiquitin-protein ligase rififylin OS=Rattus
norvegicus GN=Rffl PE=1 SV=1
Length = 362

Score = 31.6 bits (70), Expect = 3.5
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = -1

Query: 449 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 270
P ++ L+ P+ S + S LP+ Q++ + A + +TQ A+ H + D E + E
Sbjct: 156 PEQQAFLTQPQSSTV-PPTSPGLPSSPAQVTSVLAQDQETQQAIGHVSQDHEEPIFLEST 214

Query: 269 RRPPP 255
R PP
Sbjct: 215 ARVPP 219


>sp|Q37871|NINE_BP82 Protein ninE OS=Enterobacteria phage 82 GN=ninE
PE=3 SV=1
Length = 56

Score = 31.2 bits (69), Expect = 4.5
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -3

Query: 492 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTR 370
+YK N+RK EL P E+ L ++ D ++ R A+ R
Sbjct: 13 IYKVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARR 53


>sp|Q4R2Z8|ZN541_MACFA Zinc finger protein 541 (Fragment) OS=Macaca
fascicularis GN=ZNF541 PE=2 SV=2
Length = 804

Score = 27.3 bits (59), Expect(2) = 5.4
Identities = 19/70 (27%), Positives = 35/70 (50%), Gaps = 3/70 (4%)
Frame = -1

Query: 455 SSPPSKSMLSAPRESPLLM-HNSSALPA--HEQQLSRLQAINSDTQDALTHSNGDKEADL 285
S P +K L + +PL++ H+ +P H ++ Q + + +D+ KE+
Sbjct: 343 SPPGAKKPLDSTAAAPLVVPHSIPVVPVTRHVGSMAMEQEKDGEERDS-------KESSQ 395

Query: 284 DGERKRRPPP 255
+RK+RPPP
Sbjct: 396 QRKRKKRPPP 405



Score = 20.8 bits (42), Expect(2) = 5.4
Identities = 10/34 (29%), Positives = 14/34 (41%)
Frame = -2

Query: 202 GCEQHSRRKKLYALKRTLP*GIQCAQSIPSKQTS 101
GC Q R ++ + R L QC+ P S
Sbjct: 424 GCHQSRLRSPMFLVDRLLKGLFQCSPYTPPPMLS 457


>sp|A4R6X5|RT106_MAGGR Histone chaperone RTT106 OS=Magnaporthe
grisea GN=RTT106 PE=3 SV=1
Length = 468

Score = 30.8 bits (68), Expect = 5.9
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +2

Query: 512 EHLCVEIADLMCFIPSRKRYNICI 583
E L +EI D+ IP RK+YN+C+
Sbjct: 92 EELQLEIKDISVSIPQRKKYNLCL 115


>sp|P58069|RASA2_MOUSE Ras GTPase-activating protein 2 OS=Mus
musculus GN=Rasa2 PE=1 SV=1
Length = 848

Score = 30.8 bits (68), Expect = 5.9
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -2

Query: 382 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 287
CQ T+ + PGCKP T + +I ++ R+T
Sbjct: 727 CQETSESTPGCKPCTAGIPADIQIDIDEDRET 758


>sp|Q63713|RASA2_RAT Ras GTPase-activating protein 2 OS=Rattus
norvegicus GN=Rasa2 PE=2 SV=1
Length = 847

Score = 30.4 bits (67), Expect = 7.7
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2

Query: 382 CQHTNNNCPGCKPSTVTLKMLSRIQMETKRQT 287
CQ T+ PGCKP T + +I ++ R+T
Sbjct: 726 CQETSEGTPGCKPCTAGIPADIQIDIDEDRET 757


>sp|Q47270|NINE_ECOLI Protein ninE homolog from lambdoid prophage
DLP12 OS=Escherichia coli (strain K12) GN=ninE PE=3 SV=1
Length = 56

Score = 30.4 bits (67), Expect = 7.7
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = -3

Query: 492 VYKASNKRKPSMELTPFEVHALSSERITIADAQFFRFASTRT 367
+Y+ N+RK EL P E+ L ++ D ++ R A+ R+
Sbjct: 13 IYRVPNRRKRKPELKPSEIPTLLGYTASLVDKKWLRLAARRS 54


tr_hit_id B0CPT8
Definition tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (strain S238N-H82)
Align length 120
Score (bit) 40.4
E-value 0.085
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK945564|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0009_L15, 5'
(620 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor (st... 40 0.085
tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor (st... 38 0.55
tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein OS=Trypa... 37 1.2
tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein OS=Trich... 36 1.6
tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein OS=Trypa... 35 2.7
tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory trans... 35 4.7
tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative unchara... 34 6.1
tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subs... 34 6.1
tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by t... 34 6.1
tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein OS=Talar... 34 6.1
tr|A4HBI2|A4HBI2_LEIBR Putative uncharacterized protein OS=Leish... 34 7.9
tr|B6QUH3|B6QUH3_PENMA BZIP transcription factor, putative OS=Pe... 34 7.9
tr|A2R8I4|A2R8I4_ASPNC Contig An16c0230, complete genome OS=Aspe... 34 7.9

>tr|B0CPT8|B0CPT8_LACBS Predicted protein OS=Laccaria bicolor
(strain S238N-H82) GN=LACBIDRAFT_186956 PE=4 SV=1
Length = 940

Score = 40.4 bits (93), Expect = 0.085
Identities = 29/120 (24%), Positives = 54/120 (45%), Gaps = 4/120 (3%)
Frame = -1

Query: 485 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLS----RLQAINSDTQDAL 318
+H + LQ + ++ S + LL + +P ++S RL + DT+D
Sbjct: 557 RHVLDSELQQRACEFFALASRSDDDELLQNICEEMPPFPPRVSALLGRLNRKHGDTEDKR 616

Query: 317 THSNGDKEADLDGERKRRPPPLIQAHTTVRSTNWQVLSRL*ATQ*AQKAVRVEKDSPMRH 138
T +G KEA+LD E +R L++ + T S N ++ L + +++ SP +
Sbjct: 617 TWIHGGKEANLDREATKRKTFLVENNGTASSDNQGIMDSLAGLDMSSPSIQAIGSSPQEY 676


>tr|B0CW92|B0CW92_LACBS Predicted protein OS=Laccaria bicolor
(strain S238N-H82) GN=LACBIDRAFT_322427 PE=4 SV=1
Length = 194

Score = 37.7 bits (86), Expect = 0.55
Identities = 38/126 (30%), Positives = 50/126 (39%), Gaps = 4/126 (3%)
Frame = -2

Query: 544 HQIRNFDTEMLEYFARIGLQSIK*KKTFNGAH---PLRSPCSQLRENHHC*C-TILPLCQ 377
H++R + E L F + K+ N AH PLR SQ HH C T +
Sbjct: 79 HRLRRQEGEALFGFKA----KARDKQLVNNAHRRNPLRHKASQAPLPHHAMCETSMNRSD 134

Query: 376 HTNNNCPGCKPSTVTLKMLSRIQMETKRQT*MERERGDHLLLYKHTLLFAAPIGKFSVGC 197
NC GC L L R + RQT + E G H+L + ++P G S G
Sbjct: 135 RRERNCCGC----ARLLGLPR----SPRQTLVNPEEGSHILWRREHYAGSSPFGLTSSGH 186

Query: 196 EQHSRR 179
H R
Sbjct: 187 RTHFTR 192


>tr|Q4E5A5|Q4E5A5_TRYCR Putative uncharacterized protein
OS=Trypanosoma cruzi GN=Tc00.1047053506559.250 PE=4 SV=1
Length = 1559

Score = 36.6 bits (83), Expect = 1.2
Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = -1

Query: 449 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINS-DTQDALTHSNGDKEADLDGER 273
PPS S+ S PR SP L +SS+ PA ++ LQAI++ D+ D GD + E
Sbjct: 1179 PPSSSLASPPRSSPSLFSSSSS-PATDKHRRVLQAISTVDSSDVAALEKGDDSSPQAQEE 1237

Query: 272 KRRPPP 255
P
Sbjct: 1238 TAEQEP 1243


>tr|A2DSH1|A2DSH1_TRIVA Putative uncharacterized protein
OS=Trichomonas vaginalis G3 GN=TVAG_067550 PE=4 SV=1
Length = 398

Score = 36.2 bits (82), Expect = 1.6
Identities = 24/74 (32%), Positives = 37/74 (50%)
Frame = -1

Query: 449 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGERK 270
P S S S+PR+S H SSA P++ + L N D D + S+ DK G K
Sbjct: 74 PSSNSTPSSPRKSSA-KHRSSARPSNAKPLISDSKDNDDESD--SESSEDKSRPKTGGGK 130

Query: 269 RRPPPLIQAHTTVR 228
R+P +++ T ++
Sbjct: 131 RKPKKIVKKTTVIK 144


>tr|Q381C0|Q381C0_9TRYP Putative uncharacterized protein
OS=Trypanosoma brucei GN=Tb11.01.8390 PE=4 SV=1
Length = 2135

Score = 35.4 bits (80), Expect = 2.7
Identities = 19/53 (35%), Positives = 29/53 (54%)
Frame = -1

Query: 434 MLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSNGDKEADLDGE 276
+LS P SP L+ N LPA SRL ++ +A + ++GD E + +GE
Sbjct: 1038 ILSNPASSPQLLLNMCKLPAPAYMGSRLPLLSMPVHNARSDTSGDVEGETNGE 1090


>tr|Q07Q99|Q07Q99_RHOP5 Methyl-accepting chemotaxis sensory
transducer OS=Rhodopseudomonas palustris (strain BisA53)
GN=RPE_1941 PE=3 SV=1
Length = 568

Score = 34.7 bits (78), Expect = 4.7
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -1

Query: 449 PPSKSMLSAPRESPLLMHNSSALPAHEQQLSRL 351
PP + ++ A E+ L +HN S LPAH Q+L++L
Sbjct: 63 PPPEYVIEAFLEATLALHNPSNLPAHRQRLAKL 95


>tr|Q8LIH0|Q8LIH0_ORYSJ Centromere protein-like (Putative
uncharacterized protein) OS=Oryza sativa subsp. japonica
GN=OJ1200_C08.114 PE=4 SV=1
Length = 860

Score = 34.3 bits (77), Expect = 6.1
Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
Frame = -1

Query: 479 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 315
Q++ ++ +SSP S L S + P + N S P H + +RL + + +
Sbjct: 73 QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 132

Query: 314 HSNGDKEADLDGERKRRPPP 255
+ +G++EA + GER+++ P
Sbjct: 133 YIDGEQEATILGERQKQNSP 152


>tr|Q0D3H6|Q0D3H6_ORYSJ Os07g0686400 protein OS=Oryza sativa subsp.
japonica GN=Os07g0686400 PE=4 SV=1
Length = 926

Score = 34.3 bits (77), Expect = 6.1
Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
Frame = -1

Query: 479 QIKENLQWSSPPSKSML-----SAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALT 315
Q++ ++ +SSP S L S + P + N S P H + +RL + + +
Sbjct: 139 QLRRSMSFSSPAGNSFLKDRSFSFSGDVPSSLFNESDAPDHVAKSTRLTNLLNKNEVLDR 198

Query: 314 HSNGDKEADLDGERKRRPPP 255
+ +G++EA + GER+++ P
Sbjct: 199 YIDGEQEATILGERQKQNSP 218


>tr|O17969|O17969_CAEEL Protein M02G9.2, partially confirmed by
transcript evidence OS=Caenorhabditis elegans GN=M02G9.2
PE=2 SV=3
Length = 398

Score = 34.3 bits (77), Expect = 6.1
Identities = 23/65 (35%), Positives = 27/65 (41%), Gaps = 6/65 (9%)
Frame = +1

Query: 241 CACIRGGGR-LFLSPSKSASLSPFECVRAS*VSLLMACNRDN----CCS-CAGKAEELCI 402
C C G R + PS A P+ CV S L +CNR N C S C+ E C
Sbjct: 65 CVCTEGNVRNIITGPSLPALFKPYNCVSTCESSCLESCNRLNFNMKCSSVCSEACEFTCA 124

Query: 403 SNGDS 417
G S
Sbjct: 125 KQGTS 129


>tr|B8M6I9|B8M6I9_9EURO Putative uncharacterized protein
OS=Talaromyces stipitatus ATCC 10500 GN=TSTA_027460 PE=4
SV=1
Length = 266

Score = 34.3 bits (77), Expect = 6.1
Identities = 23/70 (32%), Positives = 36/70 (51%)
Frame = -1

Query: 485 KHQIKENLQWSSPPSKSMLSAPRESPLLMHNSSALPAHEQQLSRLQAINSDTQDALTHSN 306
K +++E+L SPP+ S L++P + ++ H AHE LSR + +D N
Sbjct: 27 KEKMEESLD--SPPTGSPLTSPIVTIVVGHERRLFAAHEDVLSRSAFFEAALKDQFLEPN 84

Query: 305 GDKEADLDGE 276
G K+ DL E
Sbjct: 85 G-KKVDLPDE 93