SE48 > MS01
ID SE48_MS01
Title LC–QTOF-MS/MS analysis
Instrument Waters ACQUITY UPLC System and Waters Xevo G2 QTOF mass spectrometer
Instrument Type UPLC-QTOF-MS
Ionization Method ESI
Ion Mode Positive and negative
Description LC analysis was performed on the Waters ACQUITY UPLCTM System. Samples were injected into an ACQUITY bridged ethyl hybrid (BEH) C18 column (100× 2.1 mm i.d.,1.7μm;Waters,Milford, MA, USA),and the column temperature was set at 40 ℃.The mobile phase consisted of A (0.1 % v/v formic acid in water) and B (0.1 % v/v formic acid in acetonitrile). The gradient conditions of the mobile phase were as follows: 0 min,99.5 % A; 10.0 min, 20 % A; 10.01 min, 0.5 % A;12.0 min, 0.5 % A; 12.1 min, 99.5 % A; and 14.5 min, 99.5 % A. The flow rate was 0.30 mL/min. UV–visible absorption spectra of samples were determined using a photodiode array (PDA) detector in the range of 200–600 nm. The sample injection volume was 1μL. MS detection was performed on a Waters Xevo G2 QTOF mass spectrometer with an electrospray ionization (ESI) interface (Waters). Full scan mass spectra were recorded through a range of 50–1,500 m/z. Nitrogen was used as the nebulizer and auxiliary gas; argon was utilized as the collision gas. The ESI source was operated in positive and negative ionization modes with a capillary voltage of 3 kV, sampling cone voltage of 25 V, cone gas flow of 50 L/h, desolvation gas flow of 800 L/h, desolvation temperature of 450 ℃, source temperature of 120 ℃, and CID energy ramped from 10 to 50 eV. Tandem MS analysis was performed using fast data directed analysis (FastDDA), which is rapid automated, intelligent MS/MS data acquisition for targeted qualitative analyses.
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