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NBDC - LSDB Archive
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SE55 > MS01
ID SE55_MS01
Title Metabolic profiling Analysis Using LC-ESI-MS
Instrument Waters Acquity UPLC system and Waters Q-TOF Premier
Instrument Type UPLC-QTOF-MS
Ionization Method ESI
Ion Mode Negative
Description Metabolome analysis was performed with an LC-ESI-Q-TOF/MS system equipped with an ESI interface (HPLC: Waters Acquity UPLC system; MS: Waters Q-TOF Premier) operated under previously described conditions (Matsuda et al., 2009). In the negative ion mode, the MS conditions were as follows: capillary voltage: +3.0 keV; cone voltage: 22.5 V; source temperature: 120℃; desolvation temperature: 450℃; cone gas flow: 50 L/h; desolvation gas flow: 800 L/h; collision energy: 2 V; detection mode: scan (m/z 100–2,000; dwell time: 0.45 s; interscan delay: 0.05 s, centroid); dynamic range enhancement mode: on. The scans were repeated for 19.5 min in a single run.
Comment_of_details -