|An original data file (.wiff) was converted to an unique binary file (.kiff) using in-house software (nondisclosure). Peak picking and alignment were performed using the another in-house software (nondisclosure), peaks were picked and aligned among samples automatically. By contrast with the detected m/z and migration time values of standard compounds including internal standards, peaks were annotated automatically using the same software. For normalization, the individual area of the detected peaks was divided by the peak area of the internal reference standards. Based on the calibration curves for standard compounds, peak area values were converted into values corresponding to amounts.