SE53 > S01
ID SE53_S01
Title Tomato
Organism - Scientific Name Solanum lycopersicum
Organism - ID NCBI taxonomy:4081
Compound - ID -
Compound - Source -
Preparation <Biosource species>
The two transgenic lines overexpressing milaculin gene (moneymaker
background) were also used.



<Organ specification>

Green and red fruits. A three grade color scale (green, orange, and red) was employed to evaluate tomato color.

<Growth condition>

Seedlings of Solanum lycopersicum were potted in 1/2000 a Wagner pot containing compost soil
(Kureha, Tokyo, Japan) for the soil experiment. Seeds were sown in 5 cm × 5 cm × 5 cm (height
× length × width) rockwool cubes and grown in a hydroponics system (565 mg l-1 NO-
3 , 15.7
mg l-1 NH+4
, 202.2 mg l-1 PO-
3 , 218.4 mg l-1 K+, 19.9 mg l-1 Mg+2
, 95.0 mg l-1 Ca+2
micronutrients) in an environmentally controlled growth room at 25 °C/20 °C (light/dark) and
600 ppm CO2 concentration with a light/dark cycle of 16 h/8 h for the hydroponic culture (HC)
experiment. Seedlings were placed in a netted-greenhouse located at the Gene Research Center in
University of Tsukuba

<Sampling and sampling date>

The fruits were harvested in spring (a pilot and HC experiments) and late summer (the soil experiment) in 2006, 2008, and 2009.

<Metabolism quenching method>

All samples were frozen within 30 s after sampling in liquid nitrogen. The frozen samples were lyophilized
sample_preparation_details_id SS01
Comment -
Analytical Method IDs SE53_S01_M01, SE53_S01_M02, SE53_S01_M03