| Preparation |
A Japanese rice collection of 175 accessions were used in this study (Table S1) (Yonemaru et al., 2012). The Sasanishiki/Habataki chromosome segment substitution lines (CSSLs, 39 accessions) were also used (Ando et al., 2008). Seeds were sterilized in 10% sodium hypochloric acid solution by vacuum infiltration for 1 h, and then immersed in aqueous 2% PPMTM solution (Nacalai Tesque, Kyoto, Japan, http://www.nacalai.co.jp/) at 28°C for 1 day in darkness. Seeds were sown in wet commercial fertilized soil (Bonsol II; Sumitomo Chemical, Tokyo, Japan, http://www.sumitomochem.co.jp/), and maintained under a 12-h light (28°C)/12-h dark (20°C) cycle for germination. Plants were kept under constant subirrigation conditions with tap water. After 2 weeks of growth, the entire aboveground (or aerial) part of one seedling was collected, weighed, and frozen in liquid nitrogen for analysis. Samples were stored at -80℃ until analysis. |