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DNA sequence and comparative analysis of chimpanzee chromosome 22.
Reference DNA sequence and comparative analysis of chimpanzee chromosome 22.
Watanabe H et.al
Nature. 2004 May 27;429(6990):382-8
PubMed ID: 15164055
Workflow
解析目的
ChimpChr22_Workflow_1
解析目的base substitutions analysis in region between chimpanzee chr22 and human chr21
ChimpChr22_Workflow_1_1
解析目的
入力データDNA sequences derived from the clones on the chimp chr22
入力データDNA sequences derived from the human chr21
処理内容/ 解析ツールNCBI-BLAST
出力データaligned sequences between chimp chr22 and human chr21
ChimpChr22_Workflow_1_2
解析目的
入力データaligned sequences between chimp chr22 and human chr21
データベースhuman chr21q SNP in dbSNP
処理内容/ 解析ツールmodified version of H test
出力データspecies-specific substitution pattern
ChimpChr22_Workflow_2
解析目的making human chr21q gene catalog
ChimpChr22_Workflow_2_1
解析目的
入力データDNA sequences derived from the human chr21
処理内容/ 解析ツールcut into overlapping fragments
処理条件fragment length=10kb, overlap=1kb
出力データDNA fragment sequences derived from the human chr21
ChimpChr22_Workflow_2_2
解析目的
入力データDNA fragment sequences derived from the human chr21
処理内容/ 解析ツールRepeatMasker
出力データmasked DNA fragment sequences derived from the human chr21
ChimpChr22_Workflow_2_3
解析目的
入力データmasked DNA fragment sequences derived from the human chr21
データベースdbEST
データベースnr-db
処理内容/ 解析ツールblastn
出力データpredicted gene regions in human chr21
ChimpChr22_Workflow_2_4
解析目的
入力データpredicted gene regions in human chr21
入力データcDNAs aligned to human chr21
処理内容/ 解析ツールEST_GENOME
出力データpredicted gene structures in human chr21
ChimpChr22_Workflow_3
解析目的detecting orthologs of chimp and human
ChimpChr22_Workflow_3_1
解析目的
入力データDNA sequences derived from the clones on the chimp chr22
入力データpredicted gene structures in human chr21
処理内容/ 解析ツールNCBI-BLAST
処理内容/ 解析ツールmanual process
出力データchimp chr22 regions corresponding to human chr21
出力データchimp chr22 regions not-corresponding to human chr21
ChimpChr22_Workflow_3_2
解析目的
入力データchimp chr22 regions corresponding to human chr21
入力データcDNAs aligned to human chr21
処理内容/ 解析ツールEST_GENOME
出力データdetected orthologs in chimp chr21
ChimpChr22_Workflow_3_3
解析目的
入力データchimp chr22 regions not-corresponding to human chr21
データベースnr-db (all species)
処理内容/ 解析ツールNCBI-BLAST
出力データpotential chimp chr22 specific genes
ChimpChr22_Workflow_4
解析目的multiple alignment of chimp chr22 genes with orthologs in other species
ChimpChr22_Workflow_4_1
解析目的
入力データpredicted gene structures in human chr21
処理内容/ 解析ツールconvert nucleotide to amino acid sequences
出力データpredicted polypeptides in human chr21
ChimpChr22_Workflow_4_2
解析目的
入力データpredicted polypeptides in human chr21
データベースrat, zebrafish, pufferfish(Ensembl) and ciona(JGI) polypeptides
処理内容/ 解析ツールblastp
出力データorthologs between human-rat, zebrafish, pufferfish and ciona
ChimpChr22_Workflow_4_3
解析目的
入力データorthologs between human-rat, zebrafish, pufferfish and ciona
入力データdetected orthologs in chimp chr21
入力データhuman-mouse orthologs(Gitton et al. Nature 2002)
処理内容/ 解析ツールClustalW
出力データmultiple alignments of chimp chr22 genes with orthologs in human, mouse, rat, zebrafish, pufferfish and ciona
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